Boll Damage by Southern Green Stink Bug (Hemiptera: Pentatomidae) and Tarnished Plant Bug (Hemiptera: Miridae) Caged on Transgenic Bacillus thuringiensis Cotton

Greene, Jeremy K.; Turnipseed, S. G.; Sullivan, Michael J.; Herzog, G. A.

doi:10.1093/jee/92.4.941

Cited by 101 publications

(98 citation statements)

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“…Furthermore, the plant bugs (Hemiptera: Miridae) and stink bugs (Hemiptera: Acanthosomatidae) have begun to resurge and become destructive pests in cotton fields (Hardee and Bryan, 1997;Greene et al, 1999;Wu et al, 2002;Wu, 2007) after successful control of Helicoverpa spp. and Heliothis spp.…”

Section: Introductionmentioning

confidence: 99%

Identification and tissue distribution of odorant binding protein genes in the lucerne plant bug Adelphocoris lineolatus (Goeze)

Wang

Zhang

et al. 2011

Insect Biochemistry and Molecular Biology

107

113

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Section: Introductionmentioning

confidence: 99%

Identification and tissue distribution of odorant binding protein genes in the lucerne plant bug Adelphocoris lineolatus (Goeze)

Wang

Zhang

et al. 2011

Insect Biochemistry and Molecular Biology

107

113

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“…Plant bugs (Hemiptera: Miridae) and stink bugs are destructive pests of cotton (Greene et al, 1999;Wu et al, 2002;Lu et al, 2010). Plant bugs like other insects search and locate their hosts relying on highly specific and sensitive olfaction systems to detect specific odorants for food source, oviposition sites, mating with partners and avoidance of toxins and predators (Breer et al, 1994;Field et al, 2000).…”

Section: Introductionmentioning

confidence: 99%

Functional characterization and immunolocalization of odorant binding protein 1 in the lucerne plant bug, Adelphocoris lineolatus (GOEZE)

Wang

et al. 2011

Arch Insect Biochem Physiol

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In the insect phylum, the relationships between individuals and their environment are often modulated by chemical communication. Odorant binding proteins (OBPs) are widely and robustly expressed in insect olfactory organs and play a key role in chemosensing and transporting hydrophobic odorants across the sensillum lymph to the olfactory receptor neuron. In this study, a novel OBP gene (AlinOBP1) in the lucerne plant bug, Adelphocoris lineolatus was identified, cloned and expressed. Real-time PCR results indicated that the expression level of AlinOBP1 gene differed in each developmental stage (from first instar to adult) and was predominantly expressed in the antennae of adults. The expression level of AlinOBP1 was 1.91 times higher in male antennae than in female antennae. The binding properties of AlinOBP1 with 114 odorants were measured using a fluorescence probe, N-phenyl-1-naphthylamine (1-NPN), with fluorescence competitive binding. The results revealed that AlinOBP1 exhibits high binding abilities with two major putative pheromone components, ethyl butyrate and trans-2-hexenyl butyrate. In addition, it was observed that six volatiles released from cotton, octanal, nonanal, decanal, 2-ethyl-1-hexanol, b-caryophyllene and b-ionone also bind to AlinOBP1. Immunocytochemistry analysis showed that AlinOBP1 was expressed in the sensillum lymph of sensilla trichodica and sensilla basiconca. Our results demonstrate that AlinOBP1 may function as a carrier in the chemoperception of the lucerne plant bug. C 2011 Wiley Periodicals, Inc.

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“…Plant bugs (Hemiptera: Miridae) are destructive pests of Bt cotton worldwide and cause significant yearly yield losses [35][36][37][38]. To elucidate the mechanism of insect olfactory perception and further facilitate the design and implementation of novel intervention strategies against these plant bugs, we present herein the cloning, expression, and purification of the odorant binding protein 3 of the lucerne plant bug, Adelphocoris lineolatus (Goeze).…”

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confidence: 99%

Cloning, expression and binding specificity analysis of odorant binding protein 3 of the lucerne plant bug, Adelphocoris lineolatus (Goeze)

Sun

Ren

et al. 2010

Chin. Sci. Bull.

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Credible evidence shows that odorant binding proteins (OBPs) are required for insect olfaction perception and play a key role in transporting hydrophobic odorants across the sensillum lymph to the olfactory receptors (ORs). In the present study, a novel OBP (AlinOBP3) gene from the lucerne plant bug, Adelphocoris lineolatus, was cloned and expressed. The expression pattern of AlinOBP3 was evaluated by qPCR, which indicated that AlinOBP3 was dominantly expressed in antennae. The binding properties of AlinOBP3 with 9 cotton volatiles and 5 sex pheromone analogs were measured by fluorescence competitive binding assays with the fluorescence probe 1-NPN. The results revealed that of 9 cotton volatiles, Myrcene, β-Ocimene and α-Phellandrene can bind with AlinOBP3. α-Phellandrene especially bound to AlinOBP3 with a high binding affinity, with a dissociation constant of 56.68 μmol/L. Of the 5 sex pheromone analogs, Hexyl butyrate had the strongest binding affinity with AlinOBP3, with a dissociation constant as 59.53 μmol/L. Butyl butyrate, trans-2-Hexenyl butyrate and Ethyl butyrate had medium binding affinities with AlinOBP3, with dissociation constants of 227.39, 108.77 and 143.47 μmol/L, respectively. The results suggest that AlinOBP3 might be a pheromone binding protein (PBP) with a dual-function for the perception of sex pheromones and plant volatiles. Adelphocoris lineolatus (Goeze), odorant binding protein, gene expression, Western blot, expression pattern, fluorescence competitive binding assays Citation: Gu S H, Sun Y, Ren L Y, et al. Cloning, expression and binding specificity analysis of odorant binding protein 3 of the lucerne plant bug, Adelphocoris lineolatus (Goeze).

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Boll Damage by Southern Green Stink Bug (Hemiptera: Pentatomidae) and Tarnished Plant Bug (Hemiptera: Miridae) Caged on Transgenic Bacillus thuringiensis Cotton

Cited by 101 publications

References 0 publications

Identification and tissue distribution of odorant binding protein genes in the lucerne plant bug Adelphocoris lineolatus (Goeze)

Identification and tissue distribution of odorant binding protein genes in the lucerne plant bug Adelphocoris lineolatus (Goeze)

Functional characterization and immunolocalization of odorant binding protein 1 in the lucerne plant bug, Adelphocoris lineolatus (GOEZE)

Cloning, expression and binding specificity analysis of odorant binding protein 3 of the lucerne plant bug, Adelphocoris lineolatus (Goeze)

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