Bombax ceiba flowers extract ameliorates hepatosteatosis induced by ethanol and relatively moderate fat diet in rats

Arafa, Amany A.; Foda, Doaa S; Mahmoud, Alaa; Metwally, Nadia S.; Farrag, Abdel Razik H.

doi:10.1016/j.toxrep.2019.04.008

Cited by 14 publications

(13 citation statements)

References 41 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The high and significant serum alanine aminotransferase enzyme (ALT) level detected in the rats' group injected with 8Units can be considered as a pathological case. This case reflected hepatocytes rupture which led to the release of ALT in serum (Simon-Giavarotti et al 2002;Cotran et al 2010;Arafa et al 2019). These results are in accordance with the histological findings applied on the liver tissue as shown in Fig 2b . Also decreasing levels in serum total protein were detected in this group.…”

Section: Discussionmentioning

confidence: 99%

Safety Evaluation of β-mannanase Enzyme Extracted from the Mutant Pathogenic Fungal Strain Penicillium citrinum in Female Wistar Rats

2022

Int J Vet Sci

View full text Add to dashboard Cite

External digestive exo-enzymes are enzymes used in simplifying animal food components and are now considered one of the most important top issues in the veterinary world. Introducing these exo-enzymes is believed to enhance increase in animals' body weights and help in reaching the maximum levels of digestion and absorption. The current research aimed to evaluate the safe use of the exo-enzyme β-mannanase extracted from the mutant fungal pathogen Penicillium citrinum Egy5 LC368457 before applying its use in animal feed. This was achieved by injecting daily different doses of the enzyme intraperitoneally to female rats and observing the change in body weights in addition to the determination of some hematological and biochemical parameters and the performance of histopathological examinations in rats' organs. The results showed significant retardation in body weight gain and significant changes in platelets and some immune cells count for different enzyme injected doses. An observed increase in serum ALT enzyme level was found in the high dose injected group compared to the control group. Histopathological examinations displayed the harmful impact of the high and the low doses of the injected enzyme on most of the body organs especially the liver, the kidneys, and the brain. Consequently, it can be concluded that using safe and nonpathogenic microorganisms in the extraction of exo-enzyme such as β-mannanase is an important aspect. Extraction of β-mannanase from the mutant Penicillium citrinum Egy5 LC368457 cannot be used for animal consumption but can be used for industrial purposes.

show abstract

Section: Discussionmentioning

confidence: 99%

Safety Evaluation of β-mannanase Enzyme Extracted from the Mutant Pathogenic Fungal Strain Penicillium citrinum in Female Wistar Rats

2022

Int J Vet Sci

View full text Add to dashboard Cite

show abstract

“…Methanol was replaced with the DPPH solution as a blank control (Ab), methanol was replaced with the sample as absorbance control (Ac), and Vc and BHT were used as positive controls. The inhibition rate was calculated using the following formula (1), and the results were expressed as IC 50 value (µg/mL), which represented the sample concentration to scavenge 50% free radical.…”

Section: Diphenyl-2-picrylhydrazyl Radical Scavenging Activitymentioning

confidence: 99%

“…Equivalently, methanol was replaced with the ABTS working solution as a blank control (Ab), methanol was replaced with the sample as absorbance control (Ac), Vc and BHT were used as positive controls. The scavenging rates were calculated according to equation (1), and the results were expressed as IC 50 value (µ g/mL).…”

Section: Azino-bis-(3-ethylbenzothiazoline-6-sulfonic Acid) Radical S...mentioning

confidence: 99%

“…The network pharmacology analysis was based on the results of the identification of the compounds by UPLC/Q-TOF-MS, and the information about these compounds and the compound-related targets was collected from the Traditional Chinese Medicine Systems Pharmacology Database (TCMSP), 1 PubChem database 2 and sea database 3 (31,32). By using "diabetes" as the search term, the names of diabetes target genes were obtained on the GeneCard database, 4 then the VENNY2.1 software was used to make an intersection pie chart to obtain the common target gene information of diabetes and sample.…”

Section: Network Pharmacology Analysismentioning

confidence: 99%

See 1 more Smart Citation

A preliminary study of the chemical composition and bioactivity of Bombax ceiba L. flower and its potential mechanism in treating type 2 diabetes mellitus using ultra-performance liquid chromatography quadrupole-time-flight mass spectrometry and network pharmacology analysis

et al. 2022

View full text Add to dashboard Cite

This study aimed to preliminary investigate the phytochemistry, bioactivity, hypoglycemic potential, and mechanism of action of Bombax ceiba L. flower (BCF), a wild edible and food plant in China. By using methanol extraction and liquid-liquid extraction, the crude extract (CE) of BCF and its petroleum ether (PE), dichloromethane (DCM), ethyl acetate (EtOAc), n-butanol (n-BuOH), and aqueous (AQ) fractions were obtained, and their chemical components and biological activities were evaluated. Further high-performance liquid chromatography (HPLC) analysis was carried out to identify and quantify the active constituents of BFC and its five fractions, and the phytochemical composition of the best-performing fraction was then analyzed by ultra-performance liquid chromatography quadrupole-time-flight mass spectrometry (UPLC/Q-TOF-MS). Finally, a network pharmacology strategy based on the chemical profile of this fraction was applied to speculate its main hypoglycemic mechanism. Results revealed the excellent biological activities of BCF, especially the EtOAc fraction. In addition to the highest total flavonoid content (TFC) (367.72 μg RE/mg E) and total phenolics content (TPC) (47.97 μg GAE/mg E), EtOAc showed the strongest DPPH⋅ scavenging ability (IC50 value = 29.56 μg/mL), ABTS⋅+ scavenging ability (IC50 value = 84.60 μg/mL), and ferric reducing antioxidant power (FRAP) (889.62 μg FeSO4/mg E), which were stronger than the positive control BHT. EtOAc also exhibited the second-best α-glucosidase inhibitory capacity and second-best acetylcholinesterase (AChE) inhibitory capacity with the IC50 values of 2.85 and 3.27 mg/mL, respectively. Also, EtOAc inhibited HepG2, MCF-7, Raw264.7, and A549 cell with IC50 values of 1.08, 1.62, 0.77, and 0.87 mg/mL, which were the second or third strongest in all fractions. Additionally, HPLC analysis revealed significant differences in the compounds’ abundance between different fractions. Among them, EtOAc had the most detected compounds and the highest content. According to the results of UPLC/Q-TOF-MS, 38 compounds were identified in EtOAc, including 24 phenolic acids and 6 flavonoids. Network pharmacological analysis further confirmed 41 potential targets of EtOAc in the treatment of type 2 diabetes, and intracellular receptor signaling pathways, unsaturated fatty acid, and DNA transcription pathways were the most possible mechanisms. These findings suggested that BCF was worthwhile to be developed as an antioxidant and anti-diabetic food/drug.

show abstract

“…Bark and seeds powder has hyperlipidemic activity with a reduction in serum and tissue lipid profiles. [4] The lupeol from stem bark exhibits the inhibitory effect on human umbilical venous endothelial cells (HUVECs) tube formation without affecting the growth of tumor cell lines. [5] It is reported that there are triterpenoid compounds in the stem bark extract, which could be responsible for lowering blood glucose levels.…”

mentioning

confidence: 99%

Untitled

Chikatipalli¹

2021

IJGP

View full text Add to dashboard Cite

Objective: The present study aims pharmacognostic investigations and antioxidant activity of Bombax ceiba leaf extracts. Materials and Methods: The materials and methods for standardization parameters were performed by the Association of Official Analytical Chemist (AOAC) methods. The antioxidant activity was evaluated by taking ascorbic acid as standard, total phenolic content and total flavonoid content were determined by Folin-Ciocalteu method. Results: The results of qualitative phytochemical screening of Bombax ceiba aerial part extracts showed the presence of important phytochemical compounds, namely, flavonoids, saponins, tannins, and phenolic compounds. The results of total phenolic content and total flavonoid content of different Bombax ceiba leaf extracts showed the highest TPC and TFC for pet. ether extract and the free radical scavenging activity of extracts is in concentration gradient. Conclusion: It is concluded that Bombax ceiba leaf extracts have a rich profile of phytochemicals with significant proximate composition and good antioxidant activity. The findings indicate that Bombax ceiba leaf extract can be taken as an initiative step for finding out the promising agents responsible for its phytochemical and antioxidant activities and could be an important source of natural compounds for the development of new drug.

show abstract

Bombax ceiba flowers extract ameliorates hepatosteatosis induced by ethanol and relatively moderate fat diet in rats

Abstract: Graphical abstract

Cited by 14 publications

References 41 publications

Safety Evaluation of β-mannanase Enzyme Extracted from the Mutant Pathogenic Fungal Strain Penicillium citrinum in Female Wistar Rats

Safety Evaluation of β-mannanase Enzyme Extracted from the Mutant Pathogenic Fungal Strain Penicillium citrinum in Female Wistar Rats

A preliminary study of the chemical composition and bioactivity of Bombax ceiba L. flower and its potential mechanism in treating type 2 diabetes mellitus using ultra-performance liquid chromatography quadrupole-time-flight mass spectrometry and network pharmacology analysis

Untitled

Contact Info

Product

Resources

About