Bombesin and Neurotensin Reduce Endotoxemia, Intestinal Oxidative Stress, and Apoptosis in Experimental Obstructive Jaundice

Assimakopoulos, Stelios F.; Scopa, Chrisoula D.; Zervoudakis, George; Mylonas, Panagiotis; Georgiou, Christos D.; Nikolopoulou, V.; Vagianos, Constantine

doi:10.1097/01.sla.0000149306.35717.8b

Cited by 64 publications

(41 citation statements)

References 47 publications

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“…It has been stated that a 10-day period is suffi cient for the development of OJ-related factors increasing the mortality and morbidity rates, such as intestine barrier dysfunctions and systemic endotoxemia (17). Therefore, we examined the levels of ALT, AST, AP, GGT, TB, DB levels on day 10, but also on days 3 and 7 after the operation.…”

Section: Discussionmentioning

confidence: 99%

The effect of alpha‑lipoic acid on oxidative parameters and liver injury in rats with obstructive jaundice

Atalay¹,

Özdemir²,

Tur³

et al. 2019

BLL

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INTRODUCTION: The aim of this study is to investigate the effects of obstructive jaundice on the liver and effectivity of alpha-lipoic acid on liver damage and oxidative stress. MATERIALS AND METHODS: Thirty-six male Sprague-Dawley rats were divided into 3 groups per 12 animals, namely into Group I (control group): the bile duct was only mobilized by laparotomy, Group II (bile duct ligation group-BDL): the common bile duct was closed with clips and OJ was caused after laparotomy, and Group III (bile duct ligation and alpha-lipoic acid group-BDL+LA): after closing the common bile duct, LA was administered in an intramuscular dose of 50 mg/kg for 10 days. On the 10th day, malondialdehyde, glutathione and superoxide dismutase levels were measured in liver and histopathological evaluation was performed. RESULTS: AST (U/L)/ALT(U/L) in groups I, II and III were 155.33/51.83, 445.28/165.89, 380.78/173.33, respectively (p < 0.005). Superoxide dismutase and glutathione levels were lower in patient groups than in the control group (0.31 μl/g vs 0.36 μl/g; p < 0.05). After the lipoic acid treatment, none of the biochemical markers of liver improved. Only the increase in superoxide dismutase (0.31 μl/g and 0.34 μl/g in groups II and III, respectively) and glutathione levels (0.16 μl/g and 0.22 μl/g in groups II and III, respectively) was statistically signifi cant (p < 0.05). CONCLUSIONS: Histopathological damage was statistically signifi cantly decreased and antioxidant levels were statistically signifi cantly increased after LA treatment (Tab. 1, Fig. 6, Ref. 23).

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Section: Discussionmentioning

confidence: 99%

The effect of alpha‑lipoic acid on oxidative parameters and liver injury in rats with obstructive jaundice

Atalay¹,

Özdemir²,

Tur³

et al. 2019

BLL

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“…The proliferative effect of taurodeoxycholate is at least partly mediated by regulation of the transcription of the proto-oncogene c-myc, which has been shown to play an important regulatory role during intestinal epithelial proliferation [18]. The decreased proliferative activity observed in the intestinal crypts, where the mucosal proliferation zone exists, may explain the induction of mucosal atrophy in cases of biliary obstruction [8]. On the other hand, the lack of significant differences in apoptosis between our study groups, which contradicts our previous experimental results, might be attributed to the different small intestinal segments evaluated in each study.…”

Section: Discussionmentioning

confidence: 99%

Intestinal epithelial cell proliferation, apoptosis and expression of tight junction proteins in patients with obstructive jaundice

Assimakopoulos¹,

Tsamandas²,

Louvros

et al. 2010

European Journal of Clinical Investigation

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“…When the intestinal mechanical barrier is damaged, endotoxins enter the blood circulation, causing endotoxemia. Then, the intestinal bacteria can migrate into the intestinal lymph nodes, blood, liver or spleen, causing bacterial translocation [23][24][25][26][27][28][29]. Therefore, how to protect the intestinal barrier function as well as to reduce the intestinal permeability and subsequently to decrease bacterial translocation in OJ is the key to the effective reduction of morbidity and mortality of OJ.…”

Section: Discussionmentioning

confidence: 99%

Expression of TLR2 and TLR5 in distal ileum of mice with obstructive jaundice and their role in intestinal mucosal injury

Tian

Zhang

2019

Arch Med Sci

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Introduction: The aim was to investigate the expression of TLR2 and TLR5 in the distal ileum of mice with obstructive jaundice (OJ) and their role in intestinal mucosal injury. Material and methods: A total of 100 male C57BL/6J mice were randomly assigned to two groups: (I) sham operation (SH); (II) bile duct ligation (BDL). The mice were respectively sacrificed before operation and on the 1 st , 3 rd , 5 th and 7 th days after operation to collect specimens. Various indicators were detected by PCR, immunohistochemistry and other methods. Results: TLR2 was increased gradually with the extension of OJ time in the BDL group (p < 0.05). However, the changes in the expression of TLR5 were not obvious at different time points. The amount of Bifidobacteria and Lactobacillus showed downward trends in intestinal tract of the BDL group. Furthermore, the amount of Escherichia coli was increased in intestinal tract of the BDL group. The pathological score of intestinal mucosa and the expression of NF-κB increased gradually in the BDL group with the extension of OJ time. There were positive correlations between the pathological score of intestinal mucosa and expressions of TLR2(r = 0.767, p < 0.05) and NF-κB (r = 0.817, p < 0.05) in BDL group. NF-κB expression was positively correlated with TLR2 expression(r = 0.706, p < 0.05). Conclusions: Disturbance of intestinal flora caused by OJ could increase the expression of NF-κB via up-regulating the expression of TLR2 to activate the downstream signaling pathway, thus aggravated the injury of intestinal mucosa.

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Bombesin and Neurotensin Reduce Endotoxemia, Intestinal Oxidative Stress, and Apoptosis in Experimental Obstructive Jaundice

Cited by 64 publications

References 47 publications

The effect of alpha‑lipoic acid on oxidative parameters and liver injury in rats with obstructive jaundice

The effect of alpha‑lipoic acid on oxidative parameters and liver injury in rats with obstructive jaundice

Intestinal epithelial cell proliferation, apoptosis and expression of tight junction proteins in patients with obstructive jaundice

Expression of TLR2 and TLR5 in distal ileum of mice with obstructive jaundice and their role in intestinal mucosal injury

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