Bombyx mori pylorus infection by Alphabaculovirus

Baggio, M P D; Ribeiro, L.F.C.; Vessaro-Silva, S A; Brancalhão, Rose Meire Costa

doi:10.4238/2014.april.3.3

Cited by 8 publications

(3 citation statements)

References 25 publications

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“…Ribeiro et al (2009) reported on the infection of the cardia, the transition area between the anterior and middle intestines of B. mori from the sixth dpi. Baggio et al (2014) investigated the infection of the anterior area of the posterior interstitial ring of the pylorus, which marks the transition between the middle and posterior intestine, from the fifth dpi. The occurrence of infections by pathogenic microorganisms in transitional epithelia has also been reported in humans.…”

Section: Resultsmentioning

confidence: 99%

Ampullae infection of the malpighian tubules of Bombyx mori by Alphabaculovirus

Santorum¹,

Beu²,

Ribeiro³

et al. 2017

Biosci. J.

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ABSTRACT:Alphabaculovirus is a genus of the entomopathogenic virus, whose species Bombyx mori nucleopolyhedrovirus (BmNPV) infects the silkworm, Bombyx mori, which is an important insect in the sericulture industry. A geographic isolate of BmNPV was identified in the state of Paraná, Brazil. It was infecting B. mori larvae and various organs and target tissues were identified, however, there was no information about the infection of Malpighian tubules (MT). The MT comprises the excretory system of B. mori and acts in the elimination of toxic substances and in hydroelectrolytic homeostasis. Thus, the present study examined the susceptibility and cytopathology of B. mori MT to BmNPV. To this end, hybrid fifth instar larvae were inoculated with a virus suspension at different days post-inoculation (dpi). MT segments were collected and divided into the ampullae, proximal, medial and distal regions. These were processed for light microscopy and transmission electron analysis. The MT regions revealed differences in susceptibility to BmNPV and the ampullae in its transition area was infected from the sixth dpi; the other regions did not reveal any evidence of infection. The transition area of the ampullae has not been previously described in Lepidoptera and its cytopathology revealed a hypertrophic nucleus with viroplasm, followed by the formation and development of viral polyhedra, which are common characteristics of infections by Alphabaculovirus. Thus, infection of the ampullae of the MT of B. mori by BmNPV, together with other known targets, compromises the metabolic balance of the insect, which results in consequences for silk production and damage to the sericulture sector.

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Section: Resultsmentioning

confidence: 99%

Ampullae infection of the malpighian tubules of Bombyx mori by Alphabaculovirus

Santorum¹,

Beu²,

Ribeiro³

et al. 2017

Biosci. J.

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“…In the larval stage, B. mori is particularly susceptible to BmNPV, and infection occurs mainly orally, by ingestion of food contaminated with viral polyhedra (Ribeiro et al, 2009a;Vessaro-Silva et al, 2019). BmNPV is polyorganotrophic, and several tissues have already been recognized as targets (Torquato et al, 2006a;Brancalhão et al, 2009;Ribeiro et al, 2009a;Baggio, et al, 2014;Vessaro-Silva et al, 2019).…”

Section: Introductionmentioning

confidence: 99%

Research Article Cytopathology of Bombyx mori (Lepidoptera: Bombycidae) larva integument infected by Bombyx mori nucleopolyhedrovirus

Oro¹,

Brancalhão²,

Britta³

et al. 2021

Genet. Mol. Res.

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Bombyx mori nucleopolyhedrovirus (BmNPV) is an entomopathogenic virus of the family Baculoviridae that causes disease in the silkworm, Bombyx mori, leading to a series of metabolic disorders that severely affect silk production. The main external sign of BmNPV infection can be observed in the integument of infected caterpillars, which is an important diagnostic indicator that can help direct control measures to prevent viral dispersion in rearing facilities. We analyzed the integument of B. mori caterpillars infected with BmNPV in a cytopathological study of epidermal cells and cuticle, to help identify signs of infection. Fifth instar larvae were inoculated with viral suspension and integument segments were processed for light microscopy and transmission electron microscopy. Signs of infection were monitored daily until cocooning. Cytopathology of epidermal cells showed signs of baculovirus infection, leading to cytolysis and release of viral polyhedra into the hemocoel, similar to what occurs in other BmNPV target cells. Considerable modifications were observed in the apical region of the epidermal cells, with disarrangement and involution of the microvilli, and loss of contact of the plasma membrane plaques ©FUNPEC-RP www.funpecrp.com.br Genetics and Molecular Research 20 (2): gmr18758 A.L. Oro et al. 2with the endocuticle. Plasma membrane plaques are involved in the transport of precursors for chitin synthesis and assembly of chitin myofibrils. The disorganization of the integument of silkworms infected with BmNPV shows the fragility of this organ, which easily ruptures due to infection, leading to the release of BmNPV polyhedra to the external environment, perpetuating the infection.

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“…However, infectious disease from pathogens is usually a major challenge sometimes leading to under-development, poor productivity and death of larvae. There have been previous reports of infection of silkworm larvae by various pathogens vis-à-vis: Metarhizium anisopliae (6), Alphabaculovirus (7), parasitic Nosema species (8), and Bombyx densovirus type 1 (9). Similarly, Enterococcus mundtii (E. mundtii) has been shown to be directly associated with flacherie disease of the silkworm larvae reared on artificial diet (10).…”

Section: Introductionmentioning

confidence: 99%