1992
DOI: 10.1016/0021-9673(92)80085-9
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Bonded-phase capillaries and the separation of inorganic ions by high-voltage capillary electrophoresis

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Cited by 63 publications
(15 citation statements)
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“…Consequently, it seems unlikely that many buffer ions, which tend to be weakly adsorbing, could act as a PDI and control the surface potential. However, the fact that SDS changes the EOF of PDMS, but not glass at pH 9 [28,35], indicates the tendency of hydrophobic compounds to adsorb on PDMS. The similarity of m EOF measured at 1 mM SDS at both pH 3 and 9 is in agreement with observations in polypropylene capillaries with SDS present [36], indicating adsorption of SDS regardless of the surface charge on the polymer.…”
mentioning
confidence: 95%
“…Consequently, it seems unlikely that many buffer ions, which tend to be weakly adsorbing, could act as a PDI and control the surface potential. However, the fact that SDS changes the EOF of PDMS, but not glass at pH 9 [28,35], indicates the tendency of hydrophobic compounds to adsorb on PDMS. The similarity of m EOF measured at 1 mM SDS at both pH 3 and 9 is in agreement with observations in polypropylene capillaries with SDS present [36], indicating adsorption of SDS regardless of the surface charge on the polymer.…”
mentioning
confidence: 95%
“…It was not expected that the inclusion of SDS would alter the surface of the uncoated fused-silica capillary wall since as it is negatively charged at alkaline pH, the negatively charged SDS surfactant monomers and micelles should be repelled from the wall [20]; very little if any SDS should adsorb to the capillary surface. However, it has been suggested that SDS will adsorb to the capillary surface at a level of about 20 times the CMC (the CMC of SDS in water is ca.…”
Section: Effect Of Surfactant Concentrationmentioning
confidence: 99%
“…Separations of intrinsic membrane proteins involves the added necessity of pre-solubilizing the membrane protein and keeping it solubilized during the electrophoretic run. Several approaches have been developed to reduce protein adsorption in CZE including the use of run buffers at pH extremes [4,6,8] or at high ionic strengths [13][14][15] or hydrophilic or hydrophobic run buffers serving to modify dynamically the capillary surface [4,10,13,[15][16][17][18][19][20]. Low concentrations of cationic [21][22][23][24] or non-ionic [12,21,25] or zwitterionic [22,26] surfactants act by binding or masking the silanol groups on the capillary wall.…”
Section: Introduction Capillary Zone Electrophoresis Of Proteinsmentioning
confidence: 99%
“…An ideal neutral EOF marker should be uncharged throughout the whole pH range used, should be easy to detect, and it should not significantly interact with charged components in the BGE. The most commonly used UV absorbing EOF marker in CE is mesityloxide , but phenol , adenosine , acetone, benzene, crotonaldehyde, water, paracetamol , propanone , benzyl alcohol , formamide , acrylamide , acetophenone , and DMSO have also been used. In NACE a solvent dip might be used instead of a neutral marker, although mesityloxide , iodine dissolved in formamide , acetone , anthracene , and water have been applied.…”
Section: Introductionmentioning
confidence: 99%