Bone adaptation to mechanical loading in mice is affected by circadian rhythms

Bouchard, Alice L.; Dsouza, Chrisanne; Julien, Catherine; Rummler, Maximillian; Gaumond, Marie‐Hélène; Cermakian, Nicolas; Willie, Bettina M.

doi:10.1016/j.bone.2021.116218

Cited by 18 publications

(20 citation statements)

References 74 publications

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“…Decreasing of the bone mineral density and increasing risk of patients developing osteoporosis would happen, if the circadian rhythm has disordered [ 47 ]. Bglap and Runx2 also displayed rhythmicity with the expression of Clock gene [ 48 ]. BMAL1 could influence numbers of key factors in skeletal development, such as Runx2/Osterix and HIF1α-VEGF signaling pathway [ 49 ].…”

Section: Discussionmentioning

confidence: 99%

A comprehensive analysis of the circRNA–miRNA–mRNA network in osteocyte-like cell associated with Mycobacterium leprae infection

et al. 2022

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Background Bone formation and loss are the characteristic clinical manifestations of leprosy, but the mechanisms underlying the bone remodeling with Mycobacterium leprae (M. leprae) infection are unclear. Methodology/Principal findings Osteocytes may have a role through regulating the differentiation of osteogenic lineages. To investigate osteocyte-related mechanisms in leprosy, we treated osteocyte-like cell with N-glycosylated muramyl dipeptide (N.g MDP). RNA-seq analysis showed 724 differentially expressed messenger RNAs (mRNAs) and 724 differentially expressed circular RNA (circRNAs). Of these, we filtered through eight osteogenic-related differentially expressed genes, according to the characteristic of competing endogenous RNA, PubMed databases, and bioinformatic analysis, including TargetScan, Gene Ontology, and Kyoto Encyclopedia of Genes and Genomes. Based on these results, we built a circRNA–microRNA (miRNA)–mRNA triple network. Quantitative reverse-transcription polymerase chain reaction and western blots analyses confirmed decreased Clock expression in osteocyte-like cell, while increased in bone mesenchymal stem cells (BMSCs), implicating a crucial factor in osteogenic differentiation. Immunohistochemistry showed obviously increased expression of CLOCK protein in BMSCs and osteoblasts in N.g MDP–treated mice, but decreased expression in osteocytes. Conclusions/Significance This analytical method provided a basis for the relationship between N.g MDP and remodeling in osteocytes, and the circRNA–miRNA–mRNA triple network may offer a new target for leprosy therapeutics.

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Section: Discussionmentioning

confidence: 99%

A comprehensive analysis of the circRNA–miRNA–mRNA network in osteocyte-like cell associated with Mycobacterium leprae infection

et al. 2022

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“…Significant densitometric differences are obtained in the analysis of functional or bone tissue "without activity". Bone mass is conditionally redistributed from one place to another, where force acts [2,3,12,16]. Liquid trabeculation of the mandible (large intertrabecular spaces and thin trabeculae) is a reliable sign of osteopenia [22,25].…”

Section: Discussionmentioning

confidence: 99%

Densitometric assessment in the justification of rehabilitation of patients with atrophy of the bone tissue of the mandible, on the right side

Ошурко

2022

Rep. of Morph.

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Densitometric methods for determining bone density are increasingly becoming fundamental in research and a priority in clinical applications in medical practice. Rehabilitation of patients with bone atrophy becomes impossible without assessing its density and, at the same time, is an important component in the diagnosis and planning of reconstructive surgery, in particular, prognosticity in the use of osteoplastic materials or osteointegration of dental implants. The aim of the study was to conduct a densitometric assessment to substantiate the rehabilitation of patients with atrophy of the mandible, on the right side, in the age groups of 25 to 75 years. Computed tomography digital scan scans obtained using the Vatech PaX-I 3D Green extra-oral radiography system and processed by the standardized X-ray diagnostic software Ez3D-I Original ver.5.1.9.0. Using the tools of the horizontal option panel, in particular the keys of the interface “profile”, the density of bone tissue with interpretation in conventional units of grayness (CUG) in the projection of 4.6, 4.7 teeth was investigated. To obtain qualitatively homogeneous values, we used nonparametric methods of statistical analysis of comparison of age groups – using the multidimensional Kruskal-Wallis test as an alternative intergroup analysis of variance, for simultaneous comparison of three samples. It is proved that during the statistical analysis of bone density using the Mann-Whitney U test in the studied areas of the mandible on the right, the total average number of M experimental (E) groups compared to the average number of the control group (C), high reliability in vertical (VP) measurements and confirmed p<0.05. Thus, it is proved that the early loss of the masticatory group of teeth, in the first group of studies (25-45 years), leads to high rates, with increasing bone density in the direction of distalization of the final defect of the dentition. Conversely, the lack of functional action on bone tissue in the second (46-60 years) and third (61-75 years) groups of the study, leads to a decrease in its density, and, consequently, to the devastation of the trabecular layer, which contributes to the progression of atrophic processes.

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“…Bone Surprisingly, only one study has been reported to date on the involvement of circadian oscillations in driving compression induced responses in bone. In this in vivo murine tibial loading study, cyclic compressive loading (11 N, 4 Hz, 216 cycles/day) was applied at ZT2 (light phase) or ZT14 (dark phase) for a single episode or for 2 weeks (Bouchard et al, 2022). Although compressive loading did not significantly regulate Bmal1, Clock, Per1, and Per2 transcription following a single loading episode, several downstream clock-controlled genes were differentially regulated, depending on the zeitgeber time; in mouse cortical bone loaded at ZT14, the mechanosensitive gene Sost was significantly decreased 1 and 24 h post-load whereas in mice loaded at ZT2, its expression was significantly increased 8 h post-loading.…”

Section: Skeletal Musclementioning

confidence: 99%

“…Transcription of the mechanosensitive Dkk1 was also decreased at 1 h post‐loading at ZT14, whereas increases were observed after 8 h in mice loaded at ZT2. Osteocyte markers were also differentially regulated according to the ZT time of loading including Runx2 and Bglap (osteocalcin) (ZT2, 24 h post‐load), Ctsk (cathepsin K; ZT14, 8 h post‐load), and Tnfrsf11b (osteoprotegerin) (ZT2 and 14, 8 h post‐load) (Bouchard et al, 2022). Interestingly, greater cortical bone formation was observed at ZT14 (compared to ZT2) in the midshaft region; bone remodeling was also found to be impacted more by night loading with increased periosteal resorption in mice loaded at ZT14 (Bouchard et al, 2022).…”

Section: Introductionmentioning

confidence: 99%

“…Osteocyte markers were also differentially regulated according to the ZT time of loading including Runx2 and Bglap (osteocalcin) (ZT2, 24 h post‐load), Ctsk (cathepsin K; ZT14, 8 h post‐load), and Tnfrsf11b (osteoprotegerin) (ZT2 and 14, 8 h post‐load) (Bouchard et al, 2022). Interestingly, greater cortical bone formation was observed at ZT14 (compared to ZT2) in the midshaft region; bone remodeling was also found to be impacted more by night loading with increased periosteal resorption in mice loaded at ZT14 (Bouchard et al, 2022). Analyses also indicated that the circadian effects on load‐induced bone remodeling were site‐specific, that is effects were not observed in the metaphyseal cortical nor trabecular bone, although it was speculated that these site‐specific differences might be attributed to differences in local tissue strains, fluid flow, or sclerostin abundance (Bouchard et al, 2022).…”

Section: Introductionmentioning

confidence: 99%

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Importance of mechanical cues in regulating musculoskeletal circadian clock rhythmicity: Implications for articular cartilage

Dintwa,

Hughes,

Blain

2023

Physiological Reports

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The circadian clock, a collection of endogenous cellular oscillators with an approximate 24‐h cycle, involves autoregulatory transcriptional/translational feedback loops to enable synchronization within the body. Circadian rhythmicity is controlled by a master clock situated in the hypothalamus; however, peripheral tissues are also under the control of autonomous clocks which are coordinated by the master clock to regulate physiological processes. Although light is the primary signal required to entrain the body to the external day, non‐photic zeitgeber including exercise also entrains circadian rhythmicity. Cellular mechano‐sensing is imperative for functionality of physiological systems including musculoskeletal tissues. Over the last decade, mechano‐regulation of circadian rhythmicity in skeletal muscle, intervertebral disc, and bone has been demonstrated to impact tissue homeostasis. In contrast, few publications exist characterizing the influence of mechanical loading on the circadian rhythm in articular cartilage, a musculoskeletal tissue in which loading is imperative for function; importantly, a dysregulated cartilage clock contributes to development of osteoarthritis. Hence, this review summarizes the literature on mechano‐regulation of circadian clocks in musculoskeletal tissues and infers on their collective importance in understanding the circadian clock and its synchronicity for articular cartilage mechanobiology.

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Bone adaptation to mechanical loading in mice is affected by circadian rhythms

Cited by 18 publications

References 74 publications

A comprehensive analysis of the circRNA–miRNA–mRNA network in osteocyte-like cell associated with Mycobacterium leprae infection

A comprehensive analysis of the circRNA–miRNA–mRNA network in osteocyte-like cell associated with Mycobacterium leprae infection

Densitometric assessment in the justification of rehabilitation of patients with atrophy of the bone tissue of the mandible, on the right side

Importance of mechanical cues in regulating musculoskeletal circadian clock rhythmicity: Implications for articular cartilage

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