Bone allografts combined with adipose-derived stem cells in an optimized cell/volume ratio showed enhanced osteogenesis and angiogenesis in a murine femur defect model

Wagner, Johannes M.; Conze, Nicolas; Lewik, Guido; Wallner, Christoph; Brune, J; Dittfeld, Stephanie; Jaurich, Henriette; Becerikli, Mustafa; Dadras, Mehran; Harati, Kamran; Fischer, Sebastian; Lehnhardt, Marcus

doi:10.1007/s00109-019-01822-9

Cited by 19 publications

(26 citation statements)

References 46 publications

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“…ASC and BMSC isolation was performed following modified standard protocols as described in our data article on the generation of the spectral library for this project. [25][26][27][28][29] In brief, adipose tissue was rinsed with pre-warmed (37°C) PBS (PAN Biotech, Germany). Blood vessels and connective tissue were carefully detached and discarded, before the tissue was minced.…”

Section: Cell Isolationmentioning

confidence: 99%

Comparative proteomic analysis of osteogenic differentiated human adipose tissue and bone marrow‐derived stromal cells

Dadras

May

Wagner

et al. 2020

J Cellular Molecular Medi

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Section: Cell Isolationmentioning

confidence: 99%

Comparative proteomic analysis of osteogenic differentiated human adipose tissue and bone marrow‐derived stromal cells

Dadras

May

Wagner

et al. 2020

J Cellular Molecular Medi

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“…Human adipose-derived mesenchymal stem cells (hASCs) appear to offer the same differentiation potential as MSCs; further, they are abundant in the body and are easy to isolate. Previous studies have demonstrated the osteogenic differentiation potential of hASCs when cultured in various scaffolds from inorganic and organic sources, showing that this combination successfully improved the osteogenic performance, accelerated the vascularization of the implanted area, promoted matrix mineralization, and improved bone formation [21][22][23][24]. Since hASCs are multipotent, available in large quantities per individual, and transplantable, they can be an ideal cell source for bone regeneration treatment.…”

Section: Introductionmentioning

confidence: 98%

Human Adipose-Derived Mesenchymal Stem Cells-Incorporated Silk Fibroin as a Potential Bio-Scaffold in Guiding Bone Regeneration

Sartika

Wang

Wang³

et al. 2020

Polymers

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Recently, stem cell-based bone tissue engineering (BTE) has been recognized as a preferable and clinically significant strategy for bone repair. In this study, a pure 3D silk fibroin (SF) scaffold was fabricated as a BTE material using a lyophilization method. We aimed to investigate the efficacy of the SF scaffold with and without seeded human adipose-derived mesenchymal stem cells (hASCs) in facilitating bone regeneration. The effectiveness of the SF-hASCs scaffold was evaluated based on physical characterization, biocompatibility, osteogenic differentiation in vitro, and bone regeneration in critical rat calvarial defects in vivo. The SF scaffold demonstrated superior biocompatibility and significantly promoted osteogenic differentiation of hASCs in vitro. At six and twelve weeks postimplantation, micro-CT showed no statistical difference in new bone formation amongst all groups. However, histological staining results revealed that the SF-hASCs scaffold exhibited a better bone extracellular matrix deposition in the defect regions compared to other groups. Immunohistochemical staining confirmed this result; expression of osteoblast-related genes (BMP-2, COL1a1, and OCN) with the SF-hASCs scaffold treatment was remarkably positive, indicating their ability to achieve effective bone remodeling. Thus, these findings demonstrate that SF can serve as a potential carrier for stem cells, to be used as an osteoconductive bioscaffold for BTE applications.

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“…Beyond all mentioned approaches, the use of allografts in animal models is still common. This was recently shown in pigs by the working group of Houben, using bone vascularized composite allotransplants, and Wagner and colleagues who used allografts with adipose-derived MSC and showed an enhanced osteogenesis and angiogenesis depending on the optimized cell/volume ratio [39,40]. Wang first compared umbilical cord-derived MSCs (UC-MSC) to BM-MSC due to their angiogenic effect on HUVEC in vitro, and then showed a higher angiogenic potential of UC-MSC in hind limb ischemia in mice [41].…”

Section: Angiogenesis In Bone Transplantsmentioning

confidence: 74%

Polysaccharide-Based Systems for Targeted Stem Cell Differentiation and Bone Regeneration

et al. 2019

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Bone tissue engineering is an ever-changing, rapidly evolving, and highly interdisciplinary field of study, where scientists try to mimic natural bone structure as closely as possible in order to facilitate bone healing. New insights from cell biology, specifically from mesenchymal stem cell differentiation and signaling, lead to new approaches in bone regeneration. Novel scaffold and drug release materials based on polysaccharides gain increasing attention due to their wide availability and good biocompatibility to be used as hydrogels and/or hybrid components for drug release and tissue engineering. This article reviews the current state of the art, recent developments, and future perspectives in polysaccharide-based systems used for bone regeneration.

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Bone allografts combined with adipose-derived stem cells in an optimized cell/volume ratio showed enhanced osteogenesis and angiogenesis in a murine femur defect model

Cited by 19 publications

References 46 publications

Comparative proteomic analysis of osteogenic differentiated human adipose tissue and bone marrow‐derived stromal cells

Comparative proteomic analysis of osteogenic differentiated human adipose tissue and bone marrow‐derived stromal cells

Human Adipose-Derived Mesenchymal Stem Cells-Incorporated Silk Fibroin as a Potential Bio-Scaffold in Guiding Bone Regeneration

Polysaccharide-Based Systems for Targeted Stem Cell Differentiation and Bone Regeneration

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