Bone cell responses to the composite of Ricinus communis polyurethane and alkaline phosphatase

Abstract

The aim of this study was to evaluate the response of osteoblastic cells to the composite of Ricinus communis polyurethane (RCP) and alkaline phosphatase (ALP) incubated in synthetic body fluid (SBF). RCP pure (RCPp) and RCP blended with ALP 6 mg/mL polymer (RCP+ALP) were incubated in SBF for 17 days. Four groups of RCP were tested: RCPp, RCP+ALP, and RCPp and RCP+ALP incubated in SBF (RCPp/SBF and RCP+ALP/SBF). Stem cells from rat bone marrow were cultured in conditions that allowed osteoblastic differentiation on RCP discs and were evaluated: cell adhesion, culture growth, cell viability, total protein content, ALP activity, and bone-like nodule formation. Data were compared by ANOVA or Kruskal-Wallis test. The group RCP+ALP was highly cytotoxic and, therefore, was not considered here. Cell adhesion (p = 0.14), culture growth (p = 0.39), viability (p = 0.46) and total protein content (p = 0.12) were not affected by either RCP composition or incubation in SBF. ALP activity was affected (p = 0.0001) as follows: RCPp < RCPp/SBF < RCP+ALP/SBF. Bone-like nodule formation was not observed on all evaluated groups. The composite RCP+ALP prior to SBF incubation is cytotoxic and must not be considered as biomaterial, but the incorporation of ALP to the RCP followed by SBF incubation could be a useful alternative to improve the biological properties of the RCP.

Polyurethane and PTFE membranes for guided bone regeneration: Histopathological and ultrastructural evaluation

“…In this study, the fibrous connective tissue capsule, outlining the space previously occupied by the material was permeated with IMGC and inflammatory mononuclear cells. A composite of (Ricinus communis) polyurethane (RCP) and alkaline phosphatase (ALP) incubated in synthetic body fluid (SBF) were analyzed by the response of osteoblastic cells (15). The composite RCP+ALP revealed to be cytotoxic and the authors justified that SBF incubation could be a useful alternative to improve the biological properties of the RCP.…”

Subcutaneous tissue reaction to castor oil bean and calcium hydroxide in rats

“…Other authors have demonstrated that the incorporation of alkaline phosphatase to the Ricinus communitis polyurethane followed by synthetic body fluid incubation could be a useful alternative to improve the biological properties, as bone formation, of this polyurethane 2 .…”

Polyurethane derived from Ricinus Communis as graft for bone defect treatments

“…It was demonstrated that when polyurethane is associated to calcium carbonate or calcium phosphate it can promote bone mass gain by bone matrix mineralization [15,16] . It was also observed that upon the incorporation of alkaline phosphatase to the polymer and subsequent incubation in synthetic body fluid, the biological properties of this polymer is improved [17] . When compared to the demineralized bone, it has the advantage of being reabsorbed more slowly [16] .…”