Bone Formation from Porcine Dental Germ Stem Cells on Surface Modified Polybutylene Succinate Scaffolds

Abay, Nergis; Peközer, Görke Gürel; Ramazanoğlu, Mustafa; Köse, Gamze Torun

doi:10.1155/2016/8792191

Cited by 12 publications

(5 citation statements)

References 29 publications

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“…Although most reports demonstrate that laminins can promote stem cell proliferation, some studies show that laminins have, to a certain degree, inhibitory effects on stem cell and progenitor cell proliferation, by reducing cell number and viability during expansion (Abay et al, 2016;Celebi et al, 2011;Heydarkhan-Hagvall et al, 2012;Ode et al, 2010;Qian and Saltzman, 2004). Seeger et al (2015) found that LM-211, LM-411, LM-511 and LM-521 inhibited proliferation of undifferentiated MSCs, but upon myogenic differentiation, only LM-521 significantly enhanced proliferation of myogenically differentiating cells.…”

Section: Negative Effectsmentioning

confidence: 99%

The role of laminins in cartilaginous tissues: from development to regeneration

Sun¹,

Wang²,

Toh³

et al. 2017

eCM

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As a key molecule of extracellular matrix, laminin provides a delicate microenvironment for cell functions. Recent findings suggest that laminins expressed by cartilage-forming cells (chondrocytes, progenitor cells and stem cells) could promote chondrogenesis. However, few papers outline the effect of laminins on providing a favorable matrix microenvironment for cartilage regeneration. In this review, we delineated the expression of laminins in hyaline cartilage, fibrocartilage and cartilage-like tissue (nucleus pulposus) throughout several developmental stages. We also examined the effect of laminins on the biological activities of chondrocytes, including adhesion, migration and survival. Furthermore, we scrutinized the potential influence of various laminin isoforms on cartilage-forming cells' proliferation and chondrogenic differentiation. With this information, we hope to facilitate an understanding of the spatial and temporal interactions between cartilage-forming cells and laminin microenvironment to eventually advance cell-based cartilage engineering and regeneration.

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Section: Negative Effectsmentioning

confidence: 99%

The role of laminins in cartilaginous tissues: from development to regeneration

Sun¹,

Wang²,

Toh³

et al. 2017

eCM

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“…Among those ALP, an early osteoblastic protein, expressed 28.8-fold in the experimental group compared to the control group at day 7 and then decreased throughout 21 days of incubation. Decrease in ALP expression during osteogenic differentiation through 14 and 21 days of incubation was also noticed earlier by our group. , …”

Section: Discussionmentioning

confidence: 85%

“…Decrease in ALP expression during osteogenic differentiation through 14 and 21 days of incubation was also noticed earlier by our group. 36,40 Runx2, a key transcription factor which controls the commitment of mesenchymal cells to the osteoblastic lineage, 41 was expressed highly in experimental groups. Runx2 was defined as a pro-angiogenic factor 42 as the reciprocal interactions between Runx2 and VEGF were previously shown by other groups.…”

Section: Discussionmentioning

confidence: 99%

Investigation of Vasculogenesis Inducing Biphasic Scaffolds for Bone Tissue Engineering

Peközer

Akar

Cumbul

et al. 2021

ACS Biomater. Sci. Eng.

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Vascularization is the main obstacle for the bone tissue engineering strategies since the defect size is generally large. Incorporation of angiogenic factors is one of the strategies employed in order to accelerate vascularization and improve bone healing. In this study, a biphasic scaffold consisting of fibrous poly(lactide-co-glycolide) (PLGA) and poly(lactide-co-glycolide)-block-poly(ethylene glycol)-block-poly(lactide-co-glycolide) (PLGA–PEG–PLGA) hydrogel loaded with vascular endothelial growth factor-A (VEGF) inducer, GS4012, was constructed. Mesenchymal stem cells isolated from rat bone marrow (rBMSCs) were used for differentiation into osteogenic cells, and endothelial cells isolated from rat peripheral blood (rPBECs) were used to test the in vitro endothelial cell recruitment. The biphasic scaffold was tested for cell proliferation, ALP expression, VEGF induction, expression of osteogenic genes by rBMSCs, and recruitment of rPBECs in vitro and for improved bone healing and vascularization in vivo on critical size rat cranial defects. Endothelial migration through porous insert and VEGF induction were obtained in vitro in response to GS4012 as well as the upregulation of ALP, Runx2, Col I, and OC gene expressions. The biphasic scaffold was also shown to be effective in improving endothelial cell recruitment, vascularization, and bone healing in vivo. Thus, the proposed design has a great potential for the healing of critical size bone defect in tissue engineering studies according to both in vitro and in vivo investigations.

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“…Tooth germ stem cells isolated from porcine third molars and their characterization and differentiation depending on their STRO-1 expression were first reported in detail in this paper. pTGSCs, which were isolated for this study, were also used for bone tissue engineering on fibronection or lamininmodified poly(butylene succinate) (PBS) scaffolds simultaneously with this study [12].…”

Section: Discussionmentioning

confidence: 99%

Role of STRO-1 sorting of porcine dental germ stem cells in dental stem cell-mediated bone tissue engineering

Peközer

Ramazanoğlu

Schlegel

et al. 2017

Artificial Cells, Nanomedicine, and Biotechnology

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Stem cells of dental origin emerged as a new source for the regeneration of tissues with advantages mainly including non-invasive collection procedures and lack of ethical contraversies with their harvest or use. In this study, porcine TGSCs (pTGSCs) were isolated from mandibular third molar tooth germs of 6-month-old domestic pigs. This is the first study that reports the isolation and characterization of TGSCs from porcine third molars and their differentiation depending on STRO-1 expression. PTGSCs were sorted according to their STRO-1 expression as STRO-1(+) and STRO-1(-). Sorted and unsorted heterogenous cells (US) were characterized by their osteogenic, chondrogenic and adipogenic differentiation capabilities. STRO-1(+) cells exhibited a higher proliferation rate owing to their clonogenic properties. All three groups of cells were found differentiated into osteogenic lineage as shown by ALP activity, calcium deposition assay, detection of osteogenic mRNAs and, proteins and mineralization staining. According to differentiation analysis, STRO-1(+) cells did not show a better performance for osteogenesis compared to STRO-1(-) and US cells. This might indicate that STRO-1(+) cells might require a heterogeneous population of cells including STRO-1(-) in their niche to perform their proposed role in osteogenesis.

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Bone Formation from Porcine Dental Germ Stem Cells on Surface Modified Polybutylene Succinate Scaffolds

Cited by 12 publications

References 29 publications

The role of laminins in cartilaginous tissues: from development to regeneration

The role of laminins in cartilaginous tissues: from development to regeneration

Investigation of Vasculogenesis Inducing Biphasic Scaffolds for Bone Tissue Engineering

Role of STRO-1 sorting of porcine dental germ stem cells in dental stem cell-mediated bone tissue engineering

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