Bone formation in a rat calvarial defect model after transplanting autogenous bone marrow with beta-tricalcium phosphate

Shirasu, Nobuaki; Ueno, Takaaki; Hirata, Yukari; Hirata, Azumi; Kagawa, Toshimasa; Kanou, Miwa; Wakimoto, Mari; Ota, Akiko; Imura, Hideto; Matsumura, Tatsushi; Yamada, Tomohiro; Yamachika, Eiki; Sano, Kazuo

doi:10.1016/j.acthis.2009.01.003

Cited by 30 publications

(21 citation statements)

References 16 publications

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“…In addition to being biologically active, synthetic BCP biomaterials do not lead to a risk of transmitting infectious agents or induce deleterious immune reactions contrary to allografts and xenografts. However, despite excellent osteoconductive properties, BCP remain poorly osteoinductive and almost unable to trigger bone formation or healing in non-supportive or hypotrophic areas, including rat calvaria defects [26,75]. In addition, BCP can be rapidly resorbed in certain sites, such as the human alveolar cleft, which is contaminated by nasal and oral flora [19,23].…”

Section: Discussionmentioning

confidence: 98%

Direct comparison of current cell-based and cell-free approaches towards the repair of craniofacial bone defects – A preclinical study

et al. 2015

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Section: Discussionmentioning

confidence: 98%

Direct comparison of current cell-based and cell-free approaches towards the repair of craniofacial bone defects – A preclinical study

et al. 2015

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“…32,33 Although it seems that the efficacy of regeneration supporting cells or bioactive molecules is not present in all cases, the benefits of porous scaffolds for stabilizing the defects and for providing a simple matrix for ingrowing cells are repeatedly reported. [33][34][35] Obviously, the material the scaffolds are made of plays only a minor role, as different materials such as betatricalciumphosphate, 14 hyaluronic acid, and atelocollagen 10 or a combination of hydroxyapatite and beta-tricalcium phosphate showed comparable effects. Again, there were no general beneficial effects, when these different scaffolds were used along with different cell types.…”

Section: Discussionmentioning

confidence: 99%

Bone Marrow-Derived Cell Concentrates Have Limited Effects on Osteochondral Reconstructions in the Mini Pig

Jagodzinski

Liu

Guenther

et al. 2014

Tissue Engineering Part C: Methods

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This study investigates the effects of seeding a chondrogenic and osteogenic scaffold with a bone marrowderived cell concentrate (BMCC) and reports the histological and mechanical properties 3 months after implantation in the miniature pig. Twenty defects (7 · 10 mm) were created in the femoral condyles of 10 miniature pigs. The defects were left empty (E), filled with the grafted cylinder upside down (U) or with a combined scaffold (S) containing a spongious bone cylinder (Tutobone Ò ) covered with a collagen membrane (Chondrogide Ò ). In a fourth group, the same scaffolds were implanted but seeded with a stem cell concentrate (S + BMCC). The animals were stained with calcein green after 2 weeks and xylenol orange after 4 weeks. After 3 months, the animals were sacrificed, and a mechanical analysis (Young's modulus), macroscopic, and histologic (ICRS Score) examination of the specimens was conducted. Young's modulus in the periphery was significantly lower for group E (67.5 -15.3 kPa) compared with untreated controls (171.7 -21.6 kPa, p < 0.04). Bone defects were smaller in group S (10% -8%) compared with E (27% -7%; p < 0.05). There was a trend toward smaller bony defects on comparing groups E and S + BMCC (11% -8%; p = 0.07). More red fluorescence was detected in group S + BMCC (2.3% -1.1%) compared with groups E (0.4% -0.2%) and U (0.5% -0.2%, p < 0.03). ICRS scores were higher for groups S (25.3 -3.8) and S + BMCC (26.2 -5.2, p < 0.01). In this animal model of osteochondral defects, stem cell concentrates enhance new bone apposition but fail to improve mechanical properties or histological appearance of cartilage regenerates in critical-sized defects.

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“…One study used histologic and immunohistologic evaluations to observe the course of proliferation and differentiation of bone marrow cells and osteogenesis in the defect, reporting that angiogenesis plays an important role in osteogenesis. 1 In another study, Kojima et al 2 induced osteogenesis in the calvaria of rats using a bone regeneration induction method consisting of a combination of bone-filling material atellocollagen and bovine hydroxyapatite (HA) granules and thermoplastic bioabsorbable plates. They reported that osteogenesis was promoted.…”

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confidence: 99%

Histologic Evaluation of Human Alveolar Sockets Treated With an Artificial Bone Substitute Material

Wakimoto¹,

Ueno²,

Hirata³

et al. 2011

Journal of Craniofacial Surgery

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This study involved a histologic, enzyme histologic, immunohistologic, and three-dimensional microstructure evaluating the extent of osteogenesis and repair in the human alveolar extraction socket achievable with an artificial bone substitute. After tooth extraction in 7 patients, extraction sockets were filled with Mastergraft (15% hydroxyapatite, 85% β-tricalcium phosphate complex). Radio-micrographs and histologic examinations were performed on samples obtained during dental implant placement procedure. On micro– computed tomography, new bone was observed in all collected samples, and osteogenesis was observed to have taken place around the artificial bone substitute. Histologically, active osteogenesis was found throughout the region observed. Addition of new bone around the Mastergraft was observed, and osteoblast-like cells were present. Cells that had partially invaded the artificial bone included tartrate-resistant acid phosphate–positive and CD34-positive cells. These findings indicate that the Mastergraft artificial bone induced osteogenesis in the jawbone and seemed effective for repairing bone defects.

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Bone formation in a rat calvarial defect model after transplanting autogenous bone marrow with beta-tricalcium phosphate

Cited by 30 publications

References 16 publications

Direct comparison of current cell-based and cell-free approaches towards the repair of craniofacial bone defects – A preclinical study

Direct comparison of current cell-based and cell-free approaches towards the repair of craniofacial bone defects – A preclinical study

Bone Marrow-Derived Cell Concentrates Have Limited Effects on Osteochondral Reconstructions in the Mini Pig

Histologic Evaluation of Human Alveolar Sockets Treated With an Artificial Bone Substitute Material

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