Bone formation in .BETA.-tricalcium phosphate-filled bone defects of the rat femur: Morphometric analysis and expression of bone related protein mRNA

Abstract

The purpose of the current study was to evaluate the bone formation when β-tricalcium phosphate (TCP) was implanted in bone defects of rat femurs. β-TCP granules were applied to defects created in the femurs of 65 male rats who were sacrifi ced 3, 7, 10, 14 or 30 days later. Bone tissues were embedded in paraffi n, serial sections were cut and then stained with hematoxylin-eosin. Histomorphometric analyses were also conducted. Furthermore, total mRNAs were extracted, homogenized, and reverse transcribed, after which quantitative PCR assays were conducted with a LightCycler™ using the double-stranded DNA dye Syber Green I with primers for either rat osteopontin or osteocalcin. Tissues in defects without β-TCP were used as controls. The amount of newly formed bone tissue in the β-TCP implanted group was signifi cantly greater in both the side areas and the central area of defects than in the control group. Expressions of osteopontin and osteocalcin mRNAs of cells in the defects of the experimental group were up-regulated compared with the control group at all time periods. Taken together, these results prove that β-TCP is an appropriate material for osteoconduction and promotes bone formation in bone defects.

Synthesis and in vivo evaluation of a scaffold containing wollastonite/β‐TCP for bone repair in a rabbit tibial defect model

“…As a specific group from calcium phosphate ceramics, tricalcium phosphate (TCP), used as bioceramic implants, have good rates of bioabsorption couple with its good cell‐ceramic interaction. The chemical composition of the CA 3 (PO 4 ) 2 and the Ca/P ratio of 1.5 is similar to bone ratio and even though calcium phosphate is more closely related to bone crystallography, the rate of reabsorption of TCP is about 3 to 12 times faster than common ceramics (Shiratori et al, ).…”

Association of vitamin D3 with alveolar bone regeneration in dogs

“…Synthetic bone grafts were referred to serve as scaffolds for bone formation, maintain space for bone growth, and promote wound healing by stabilizing blood clots 2. However, the osseoinduction potential by using synthetic bone grafts alone is limited 3.…”

Synthesis and cytotoxicity evaluation of granular magnesium substituted β-tricalcium phosphate

“…In vivo studies with β-TCP implanted in the rat femoral condyle were conducted by Kondo, et al 14 (2005), concluding that β-TCP has a good biocompatibility, since both bioresorption and bone formation started at an early phase after implantation. Shiratori, et al 26 (2005) also considered β-TCP an osteoconductive biomaterial, based on the histological and molecular findings of bone tissue withdrawn from bone defects in rat femurs previously implanted with β-TCP granules.…”