Bone growth in biomimetic apatite coated porous Polyactive® 1000PEGT70PBT30 implants

Du, Chang; Meijer, G.J.; Valk, C van de; Haan, RE; Bezemer, J.M.; Hesseling, S. C.; Cui, Fuzhai; Groot, K. de; Layrolle, Pierre

doi:10.1016/s0142-9612(02)00214-4

Cited by 63 publications

(51 citation statements)

References 26 publications

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“…3D scaffolds made from 300/55/45 and 1000/70/30 compositions (Fig. 1A) were produced using either a CM and particle leaching technique [25] or a 3DF deposition technique [19,20]. Briefly, CM scaffolds were prepared by mixing PEGT/ PBT granules with sodium chloride grains (75% by volume), sieved to obtain particles ranging in size from 400 to 600 mm.…”

Section: Scaffold Fabricationmentioning

confidence: 99%

Effects of scaffold composition and architecture on human nasal chondrocyte redifferentiation and cartilaginous matrix deposition

et al. 2005

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Section: Scaffold Fabricationmentioning

confidence: 99%

Effects of scaffold composition and architecture on human nasal chondrocyte redifferentiation and cartilaginous matrix deposition

et al. 2005

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“…Previously, PEOT/PBT copolymers have been extensively studied and proved to be suitable for use as scaffold material in tissue engineering, both in vitro and in vivo [22][23][24][25], and reached clinical applications (PolyActive, IsoTis Orthopaedics SA) as dermal substitutes [26] and bone fillers [27,28].…”

Section: Introductionmentioning

confidence: 99%

Label-free Raman monitoring of extracellular matrix formation in three-dimensional polymeric scaffolds

Leferink

Okagbare

Morris

et al. 2013

J. R. Soc. Interface.

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Monitoring extracellular matrix (ECM) components is one of the key methods used to determine tissue quality in three-dimensional scaffolds for regenerative medicine and clinical purposes. Raman spectroscopy can be used for noninvasive sensing of cellular and ECM biochemistry. We have investigated the use of conventional (confocal and semiconfocal) Raman microspectroscopy and fibre-optic Raman spectroscopy for in vitro monitoring of ECM formation in three-dimensional poly(ethylene oxide terephthalate)-poly(butylene terephthalate) (PEOT/PBT) scaffolds. Chondrocyte-seeded PEOT/PBT scaffolds were analysed for ECM formation by Raman microspectroscopy, biochemical analysis, histology and scanning electron microscopy. ECM deposition in these scaffolds was successfully detected by biochemical and histological analysis and by label-free non-destructive Raman microspectroscopy. In the spectra collected by the conventional Raman set-ups, the Raman bands at 937 and at 1062 cm 21 which, respectively, correspond to collagen and sulfated glycosaminoglycans could be used as Raman markers for ECM formation in scaffolds. Collagen synthesis was found to be different in single chondrocyte-seeded scaffolds when compared with microaggregate-seeded samples. Normalized band-area ratios for collagen content of single cell-seeded samples gradually decreased during a 21-day culture period, whereas collagen content of the microaggregate-seeded samples significantly increased during this period. Moreover, a fibre-optic Raman set-up allowed for the collection of Raman spectra from multiple pores inside scaffolds in parallel. These fibre-optic measurements could give a representative average of the ECM Raman signal present in tissue-engineered constructs. Results in this study provide proofof-principle that Raman microspectroscopy is a promising non-invasive tool to monitor ECM production and remodelling in three-dimensional porous cartilage tissue-engineered constructs.

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“…Highly interconnected porous structures provide a large surface area, enabling cells to migrate, proliferate and differentiate [15,16]. The in-house produced BCP ceramic had a highly interconnected macroand microporous structure, and was previously shown to be both osteoconductive and osteoinductive [17][18][19][20].…”

Section: Introductionmentioning

confidence: 99%

Biological performance in goats of a porous titanium alloy–biphasic calcium phosphate composite

Li¹,

Habibović²,

Yuan³

et al. 2007

Biomaterials

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Bone growth in biomimetic apatite coated porous Polyactive® 1000PEGT70PBT30 implants

Cited by 63 publications

References 26 publications

Effects of scaffold composition and architecture on human nasal chondrocyte redifferentiation and cartilaginous matrix deposition

Effects of scaffold composition and architecture on human nasal chondrocyte redifferentiation and cartilaginous matrix deposition

Label-free Raman monitoring of extracellular matrix formation in three-dimensional polymeric scaffolds

Biological performance in goats of a porous titanium alloy–biphasic calcium phosphate composite

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