Bone healing after bone marrow stromal cell transplantation to the bone defect

Niedźwiedzki, Tadeusz; Dabrowski, Z; Miszta, H; Pawlikowski, M.

doi:10.1016/0142-9612(93)90221-m

Cited by 47 publications

(31 citation statements)

References 23 publications

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“…Cells expressing the gene were obtained from a line of transgenic mice (line 73) in which the copy number of the human mini-gene relative to the endogenous mouse gene was -100:1, and the steady-state levels of mRNA from the human mini-gene relative to mRNA from the endogenous mouse gene was -0.5:1 in most tissues (19)(20)(21). Donor cells from marrow partially enriched for mesenchymal precursors were prepared by using protocols (6)(7)(8)(9)(10)(11)(12)(13)(14)(15)(16)(17) in which marrow cells that tightly adhere to plastic were isolated and grown in culture in the presence of FBS but in the absence of growth factors that favor the replication of hematopoietic precursors. As reported (6,11,12), foci containing two to four fibroblast-like cells appeared in 2-3 days, and the foci grew rapidly into colonies over 7-10 days.…”

Section: Resultsmentioning

confidence: 99%

“…[1][2][3][4][5]. In addition to hematopoietic stem cells, marrow contains mesenchymal precursor cells (6) that produce fibrous tissue, bone, or cartilage when implanted into appropriate tissues in vivo (7)(8)(9)(10) and that generate colonies of fibroblastic (6,(11)(12)(13)(14)(15)(16)(17), adipocytic (14), and osteogenic (14) cells when cultured under appropriate conditions. One of the most distinctive features of mesenchymal cells that synthesize bone, cartilage, and other connective tissues is their high level of expression of genes for specific types of collagen (see ref.…”

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confidence: 99%

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Cultured adherent cells from marrow can serve as long-lasting precursor cells for bone, cartilage, and lung in irradiated mice.

Pereira

Halford

O'Hara

et al. 1995

Proc. Natl. Acad. Sci. U.S.A.

830

580

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Cells from transgenic mice expressing a human mini-gene for collagen I were used as markers to follow the fate of mesenchymal precursor cells from marrow that were partially enriched by adherence to plastic, expanded in culture, and then injected into irradiated mice. Sensitive PCR assays for the marker collagen I gene indicated that few of the donor cells were present in the recipient mice after 1 week, but 1-5 months later, the donor cells accounted for 1.5-12% of the cells in bone, cartilage, and lung in addition to marrow and spleen. A PCR in situ assay on lung indicated that the donor cells diffusely populated the parenchyma, and reverse transcription-PCR assays indicated that the marker collagen I gene was expressed in a tissue-specific manner. The results, therefore, demonstrated that mesenchymal precursor cells from marrow that are expanded in culture can serve as long-lasting precursors for mesenchymal cells in bone, cartilage, and lung. They suggest that cells may be particularly attractive targets for gene therapy ex vivo.

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Section: Resultsmentioning

confidence: 99%

mentioning

confidence: 99%

Cultured adherent cells from marrow can serve as long-lasting precursor cells for bone, cartilage, and lung in irradiated mice.

Pereira

Halford

O'Hara

et al. 1995

Proc. Natl. Acad. Sci. U.S.A.

830

580

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“…healing in long bone fractures when locally delivered to the fracture site in several species [8,10,26]. MSCs administered intravenously have been shown to engraft into bone marrow tissues and bone preferentially [20,30].…”

Section: Shirley Et Al I Journal Of Orthopuedic Research 23 (2005mentioning

confidence: 99%

Systemic recruitment of osteoblastic cells in fracture healing

Shirley

Marsh

Jordan

et al. 2005

Journal Orthopaedic Research

129

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We hypothesise that following a bone fracture there is systemic recruitment of bone forming cells to a fracture site. A rabbit ulnar osteotomy model was adapted to trace the movement of osteogenic cells. Bone marrow mesenchymal stem cells from 41 NZW rabbits were isolated, culture-expanded and fluorescently labelled. The labelled cells were either re-implanted into the fracture gap (Group A); into a vein (Group B); or into a remote tibia1 bone marrow cavity 48 h after the osteotomy (Group C ) or 4 weeks before the osteotomy was established (Group D), and a control group (Group E) had no labelled cells given. To quantify passive leakage of cells to an injury site, inert beads were also co-delivered in Group B. Samples of the fracture callus tissue and various organs were harvested at discrete sacrifice time-points to trace and quantify the labelled cells. At 3 weeks following osteotomy, the number of labelled cells identified in the callus of Group C , was significantly greater than following IV delivery, Group B, and there was no difference in the number of labelled cells in the callus tissues, between Groups C and A, indicating the labelled bone marrow cells were capable of migrating to the fracture sites from the remote bone marrow cavity. Significantly fewer inert beads than labelled cells were identified in Group B callus, suggesting some of the bone-forming cells were actively recruited and selectively chosen to the fracture site, rather than passively leaked into the circulation and to bone injury site. This investigation supports the hypothesis that some osteoblasts involved in fracture healing were systemically mobilised and recruited to the fracture from remote bone marrow sites.

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“…Moreover, hBMSCs may play important roles during the initial processes of fracture healing and skeletal repair [13].…”

Section: Introductionmentioning

confidence: 99%

Osteogenic abilities of bone marrow stromal cells are not defective in patients with osteonecrosis

Yoo

Song²,

Koo

et al. 2008

International Orthopaedics (SICOT)

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We investigated whether the proliferation activities and osteogenic capacities of bone marrow stromal cells (BMSCs) are depressed in patients with osteonecrosis of the femoral head (ONFH). BMSCs were isolated from fresh bone marrow of the iliac crests of 54 donors and were differentiated into osteogenic lineage in vitro. The results of 27 consecutive patients with ONFH (16 idiopathic and 11 alcohol-induced) were compared with those of 27 patients with a nonnecrotic hip disorder. The proliferative activities of BMSCs in patients with ONFH were not found to be reduced and their osteogenic capacities (alkaline phosphatase activity and calcium deposition amount) were unaltered during in vitro differentiation. Results from patients with idiopathic or alcohol-induced ONFH were similar to those of matched patients with nonnecrotic disorder. These findings suggest that the osteogenic potentials of BMSCs are not defective in patients with ONFH.

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Bone healing after bone marrow stromal cell transplantation to the bone defect

Cited by 47 publications

References 23 publications

Cultured adherent cells from marrow can serve as long-lasting precursor cells for bone, cartilage, and lung in irradiated mice.

Cultured adherent cells from marrow can serve as long-lasting precursor cells for bone, cartilage, and lung in irradiated mice.

Systemic recruitment of osteoblastic cells in fracture healing

Osteogenic abilities of bone marrow stromal cells are not defective in patients with osteonecrosis

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