Bone healing in critical‐size defects treated with platelet‐rich plasma: a histologic and histometric study in rat calvaria

Messora, Michel Reis; Nagata, Maria José Hitomi; Mariano, Ronaldo Célio; Dornelles, Rita Cássia Menegati; Bomfim, Suely Regina Mogami; Fucini, Stephen E.; Garcia, Valdir Gouveia; Bosco, Álvaro Francisco

doi:10.1111/j.1600-0765.2007.01017.x

Cited by 72 publications

(67 citation statements)

References 42 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…This result is similar to those reported elsewhere in rats, 74 although there are multiple reports in humans 75 and animals, 76 including rats, 77,78 of PRP enhancing bone formation. Still, evaluating the efficacy of PRP remains complicated.…”

supporting

confidence: 81%

Uncultured Marrow Mononuclear Cells Delivered Within Fibrin Glue Hydrogels to Porous Scaffolds Enhance Bone Regeneration Within Critical-Sized Rat Cranial Defects

Kretlow

Spicer

Jansen

et al. 2010

Tissue Engineering Part A

View full text Add to dashboard Cite

For bone tissue engineering, the benefits of incorporating mesenchymal stem cells (MSCs) into porous scaffolds are well established. There is, however, little consensus on the effects of or need for MSC handling ex vivo. Culture and expansion of MSCs adds length and cost, and likely increases risk associated with treatment. We evaluated the effect of using uncultured bone marrow mononuclear cells (bmMNCs) encapsulated within fibrin glue hydrogels and seeded into porous scaffolds to regenerate bone over 12 weeks in an 8-mm-diameter, criticalsized rat cranial defect. A full factorial experimental design was used to evaluate bone formation within model poly(L-lactic acid) and corraline hydroxyapatite scaffolds with or without platelet-rich plasma (PRP) and bmMNCs. Mechanical push-out testing, microcomputed tomographical analyses, and histology were performed. PRP showed no benefit for bone formation. Cell-laden poly(L-lactic acid) scaffolds without PRP required significantly greater force to displace from surrounding tissues than control (cell-free) scaffolds, but no differences were observed during push-out testing of coral scaffolds. For bone volume formation as analyzed by microcomputed tomography, significant positive overall effects were observed with bmMNC incorporation. These data suggest that bmMNCs may provide therapeutic advantages in bone tissue engineering applications without the need for culture, expansion, and purification.

show abstract

supporting

confidence: 81%

Uncultured Marrow Mononuclear Cells Delivered Within Fibrin Glue Hydrogels to Porous Scaffolds Enhance Bone Regeneration Within Critical-Sized Rat Cranial Defects

Kretlow

Spicer

Jansen

et al. 2010

Tissue Engineering Part A

View full text Add to dashboard Cite

show abstract

“…The influential time of the growth factors of the PRP is up to 6-7 months. 13 In our study, the consolidation of the graft was earlier in Group B patients in whom the ABS was mixed with PRP which was based on the premise that the large number of platelets in PRP release significant quantities of growth factors that aid bone graft maturation in accordance with Consolo et al, 13 Marx, 14 Green David and Klink 15 and Messora et al, 16 but contradictory to Shanaman et al, 17 Arpornmaeklong et al, 18 de Vasconelos et al 19 and enhances bone regeneration. Advantages of using an autologous PRP include no risk of cross-reactivity, immune reaction or disease transmission.…”

Section: Discussionsupporting

confidence: 57%

Role of platelet-rich plasma in combination with alloplastic bone substitute in regeneration of osseous defects

Singh¹,

Gupta²,

Pradhan³

et al. 2011

Journal of Oral Biology and Craniofacial Research

View full text Add to dashboard Cite

Introduction: Bone grafts are frequently used for the treatment of bone defects, but can cause postoperative complications, and sometimes a sufficient quantity of bone is not available. Hence, synthetic biomaterials have been used as an alternative to autogenous bone grafts. Recent clinical reports suggest that application of autologous blood plasma enriched with platelets can enhance the formation of new bone. There are very few in vitro or in vivo studies published on the efficiency of platelet-rich plasma (PRP). The objective of this study was to evaluate the alloplastic bone substitute for its osteogenic potential with or without PRP.

show abstract

“…The following criteria, based on the work of Messora et al, 7 were used to standardize the histomorphometric analysis of the digital images: (1) the total area (TA) to be analyzed corresponded to the entire area of the original defect. This area was determined by first identifying the external and internal surfaces of the original calvarium at the right and left margins of the surgical defect, and then connecting them with lines drawn following their respective curvatures.…”

Section: Histomorphometric Analysismentioning

confidence: 99%

“…2). 7 Following complete decalcification, they were processed and embedded in paraffin. Serial sections 6 mmthick were cut in a longitudinal direction starting at the center of the original surgical defect.…”

Section: Tissue Processingmentioning

confidence: 99%

See 1 more Smart Citation

Influence of the ratio of particulate autogenous bone graft/platelet-rich plasma on bone healing in critical-size defects: A histologic and histometric study in rat calvaria

et al. 2009

View full text Add to dashboard Cite

The purpose of this study was to analyze histomorphometrically the influence of the ratio of particulate autogenous bone (AB) graft/platelet-rich plasma (PRP) on bone healing in surgically created critical-size defects (CSD) in rat calvaria. Fifty rats were divided into five groups: Group C (control), Group AB, Group AB/PRP-50, Group AB/PRP-100, and Group AB/PRP-150. A 5-mm diameter critical-size defect was created in the calvarium of each animal. In Group C, the defect was filled by blood clot only. In Group AB, the defect was filled with 0.01 mL of AB graft. In Groups AB/PRP-50, AB/PRP-100, and AB/PRP-150, the defects were filled with 0.01 mL of AB graft combined with 50, 100, and 150 mL of PRP, respectively. All animals were euthanized at 30 days postoperative. Histomorphometry, using image analysis software, and histology analyses were performed. New Bone Area (NBA) and the remaining bone graft particles area (RPA) were calculated as a percentage of the total area of the original defect. Percentage data were transformed into arccosine for analysis. No defect completely regenerated with bone. Group AB/PRP-50 (41.78 AE 13.48%) had a significantly greater NBA than Groups C (19.29 AE 5.11%), AB (27.43 AE 10.90%) or AB/PRP-150 (19.17 AE 8.45%) (p < 0.05). No significant differences were observed between groups AB/PRP-50 and AB/ PRP-100 or among groups AB, AB/PRP-100, and AB/PRP-150 with regard to NBA (p > 0.05). Group AB/PRP-150 (31.59 AE 3.22%) had a significantly greater RPA than Groups AB (19.09 AE 5.21%), AB/PRP-50 (17.33 AE 4.43%), and AB/PRP-100 (19.72 AE 3.62%) (p < 0.001). No significant differences were observed among groups AB, AB/PRP-50, and AB/PRP-100 with regard to RPA (p > 0.05). The ratio AB graft/PRP influences bone healing in surgically created CSD in rat calvaria. ß

show abstract

Bone healing in critical‐size defects treated with platelet‐rich plasma: a histologic and histometric study in rat calvaria

Abstract: Within the limits of this study, it can be concluded that platelet-rich plasma placed in the defects and covered by platelet-poor plasma significantly enhanced bone healing in critical-size defects in rat calvaria.

Cited by 72 publications

References 42 publications

Uncultured Marrow Mononuclear Cells Delivered Within Fibrin Glue Hydrogels to Porous Scaffolds Enhance Bone Regeneration Within Critical-Sized Rat Cranial Defects

Uncultured Marrow Mononuclear Cells Delivered Within Fibrin Glue Hydrogels to Porous Scaffolds Enhance Bone Regeneration Within Critical-Sized Rat Cranial Defects

Role of platelet-rich plasma in combination with alloplastic bone substitute in regeneration of osseous defects

Influence of the ratio of particulate autogenous bone graft/platelet-rich plasma on bone healing in critical-size defects: A histologic and histometric study in rat calvaria

Contact Info

Product

Resources

About