Bone healing in critical‐size defects treated with platelet‐rich plasma activated by two different methods. A histologic and histometric study in rat calvaria

Messora, Michel Reis; Nagata, Maria José Hitomi; Dornelles, Rita Cássia Menegati; Bomfim, Suely Regina Mogami; Furlaneto, Flávia Aparecida Chaves; Melo, Luiz Gustavo Nascimento de; Deliberador, Tatiana Miranda; Bosco, Álvaro Francisco; Garcia, Valdir Gouveia; Fucini, Stephen E.

doi:10.1111/j.1600-0765.2008.01084.x

Cited by 64 publications

(62 citation statements)

References 33 publications

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“…5). For the sham operation, these results were very similar to the results reported by Messora et al 20) . Fukushima et al 7) reported that DNA/protamine complex paste gradually degraded after implantation into the soft tissue of rats, and deep purple complex fragments were observed 3 days after implantation, and had completely disappeared from the tissues within 10 days after implantation.…”

Section: Histological Evaluationsupporting

confidence: 82%

“…4, treatment with DNA/protamine complex paste showed a significantly higher bone regeneration areas than the sham operation. Messora et al 20) reported that newly formed bone areas in a calvarial defect of 8 mm were approximately 6 % and 12% one month and 3 months after the sham operation, respectively. Their results were very similar to the results obtained from the present study.…”

Section: Histological Evaluationmentioning

confidence: 99%

“…19) . In the present study, a calvarial defect with 8 mm was created for the animal examination because this bone defect model has been used as a critical size in rat calvarial bone defects 20,21) . Bone formation in the calvarial defect model occurs under unloaded conditions.…”

Section: Histological Evaluationmentioning

confidence: 99%

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Osteogenic Evaluation of DNA/Protamine Complex Paste in Rat Cranial Defects

Shinozaki

Yanagi

Yamaguchi

et al. 2013

J. Hard Tissue Biology.

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A DNA/protamine complex powder, prepared from the reaction between DNA and protamine sulfate solutions, was mixed with water to make a paste. The ALP activity of MC 3T3 E1 cultured on a thin film of the complex paste was higher than that cultured on a plastic well. It was found that DNA/protamine complex induced new bone formation from the results of micro-computed tomography ( -CT) images and conventional histological sections with hematoxylin and eosin staining in rat cranial defect tests. -CT image analyses showed that newly formed bone areas in defects for DNA/protamine complex were significantly higher than those for sham operation (controls) two and three months after surgery. Although the area of red-appearing osteoids reduced with increasing times after surgery, stimulated new bone formation areas were observed around newly formed bone in histological sections stained with Villanuva osteochrome bone stain. Therefore, DNA/protamine complex paste with a biodegradable property will be a useful injectable biomaterial for the repair of bone defects.

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Section: Histological Evaluationsupporting

confidence: 82%

Section: Histological Evaluationmentioning

confidence: 99%

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Osteogenic Evaluation of DNA/Protamine Complex Paste in Rat Cranial Defects

Shinozaki

Yanagi

Yamaguchi

et al. 2013

J. Hard Tissue Biology.

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“…Several aspects were considered to guarantee their integrity in the PRP samples, such as the choice of the anticoagulant, the speed of the centrifugation, the activator used to initiate PRP clot formation, and the amount of time between activation of the PRP and its clinical use. 24,[30][31][32] According to Marx, 3 platelets damaged or rendered nonviable by PRP processing will not secrete bioactive GFs, and may result in disappointing outcomes.…”

Section: Discussionmentioning

confidence: 99%

Influence of the ratio of particulate autogenous bone graft/platelet-rich plasma on bone healing in critical-size defects: A histologic and histometric study in rat calvaria

et al. 2009

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The purpose of this study was to analyze histomorphometrically the influence of the ratio of particulate autogenous bone (AB) graft/platelet-rich plasma (PRP) on bone healing in surgically created critical-size defects (CSD) in rat calvaria. Fifty rats were divided into five groups: Group C (control), Group AB, Group AB/PRP-50, Group AB/PRP-100, and Group AB/PRP-150. A 5-mm diameter critical-size defect was created in the calvarium of each animal. In Group C, the defect was filled by blood clot only. In Group AB, the defect was filled with 0.01 mL of AB graft. In Groups AB/PRP-50, AB/PRP-100, and AB/PRP-150, the defects were filled with 0.01 mL of AB graft combined with 50, 100, and 150 mL of PRP, respectively. All animals were euthanized at 30 days postoperative. Histomorphometry, using image analysis software, and histology analyses were performed. New Bone Area (NBA) and the remaining bone graft particles area (RPA) were calculated as a percentage of the total area of the original defect. Percentage data were transformed into arccosine for analysis. No defect completely regenerated with bone. Group AB/PRP-50 (41.78 AE 13.48%) had a significantly greater NBA than Groups C (19.29 AE 5.11%), AB (27.43 AE 10.90%) or AB/PRP-150 (19.17 AE 8.45%) (p < 0.05). No significant differences were observed between groups AB/PRP-50 and AB/ PRP-100 or among groups AB, AB/PRP-100, and AB/PRP-150 with regard to NBA (p > 0.05). Group AB/PRP-150 (31.59 AE 3.22%) had a significantly greater RPA than Groups AB (19.09 AE 5.21%), AB/PRP-50 (17.33 AE 4.43%), and AB/PRP-100 (19.72 AE 3.62%) (p < 0.001). No significant differences were observed among groups AB, AB/PRP-50, and AB/PRP-100 with regard to RPA (p > 0.05). The ratio AB graft/PRP influences bone healing in surgically created CSD in rat calvaria. ß

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“…The quantity and quality of bone that forms in an osseous defect is influenced by the type and age of the animal, the stability and size of the defect, and other factors 36) . In the present study, a calvarial defect of 8 mm in diameter was created for the animal examination because it had been previously demonstrated to be a critical size for rat calvarial bone defects 37,38) . Bone formation in the calvarial defect model occurs under unloaded conditions.…”

Section: Discussionmentioning

confidence: 99%

Biological Evaluation of a Prototype Material made of Polyglycolic Acid and Hydroxyapatite

Takahashi

Yamaguchi

Tanimoto

et al. 2015

J. Hard Tissue Biology.

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Orthodontic anchor screws, which made from titanium are widely used as anchorage units in orthodontic treatment. However, there are some problems, including dropout during treatment and the invasive nature of screw removal. An orthodontic anchor screw, in which the main component is a bioabsorbable material is therefore sought. We investigated the biological evaluation of a prototype material composed of polyglycolic acid (PGA) and hydroxyapatite (HAp). Five types of PGA/HAp composites were examined. The HAp compound ratios by weight were 0, 5, 10, 30 and 50 %. We examined the effects of the PGA/HAp components on the gene and protein expression of collagen type 1 (COL-1), alkaline phosphatase (ALP) and osteocalcin (OCN) from mouse osteoblasts (MC3T3-E1) in vitro by real-time a PCR and an ELISA. Furthermore, we evaluated the osteoinductive potential in vivo, in a rat calvarial defect model after subjecting samples to a -CT image analysis. COL-1 and ALP production peaked on day 7, and increased dependent on the concentration of HAp. OCN production also increased in a dose-and time dependent manner up to day 10 in vitro. In the in vivo study, -CT image analyses showed that newly formed bone areas in defects for PGA/HAp disk were significantly higher at two and three months after surgery than in controls that underwent sham operations. The results suggest that the biological properties of the PGA-based trial prototype were increased with the addition of HAp. An orthodontic anchor screw constructed of PGA may be useful for orthodontists.

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Bone healing in critical‐size defects treated with platelet‐rich plasma activated by two different methods. A histologic and histometric study in rat calvaria

Abstract: It can be concluded that the type of activator used to initiate PRP clot formation influences its biological effect on bone healing in CSD in rat calvaria.

Cited by 64 publications

References 33 publications

Osteogenic Evaluation of DNA/Protamine Complex Paste in Rat Cranial Defects

Osteogenic Evaluation of DNA/Protamine Complex Paste in Rat Cranial Defects

Influence of the ratio of particulate autogenous bone graft/platelet-rich plasma on bone healing in critical-size defects: A histologic and histometric study in rat calvaria

Biological Evaluation of a Prototype Material made of Polyglycolic Acid and Hydroxyapatite

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