Bone healing with oxytocin‐loaded microporous β‐<scp>TCP</scp>bone substitute in ectopic bone formation model and critical‐sized osseous defect of rat

Park, Jin Woo; Kim, Jae-Min; Lee, Heon-Jin; Jeong, Seong-Hwa; Suh, Jo‐Young; Hanawa, Takao

doi:10.1111/jcpe.12198

Cited by 18 publications

(14 citation statements)

References 51 publications

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“…). New bone area was calculated and expressed as a percentage of new bone area within total area of calvarial defect created by trephination according to the method described by Park et al. (). Biomaterial residues were calculated and expressed as a percentage of the residual biomaterial area within total defect area (Park et al. ). Height of reconstruction was determined in mm as the maximal thickness of the new tissue/biomaterial compound in the central third of the former defect (Schmidlin et al.…”

Section: Methodsmentioning

confidence: 99%

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Effect of poly (lactide‐co‐glycolide) (PLGA)‐coated beta‐tricalcium phosphate on the healing of rat calvarial bone defects: a comparative study with pure‐phase beta‐tricalcium phosphate

Bizenjima

Takeuchi

Seshima

et al. 2016

Clinical Oral Implants Res

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Section: Methodsmentioning

confidence: 99%

“…2. New bone area was calculated and expressed as a percentage of new bone area within total area of calvarial defect created by trephination according to the method described by Park et al (2014). 3.…”

Section: Histomorphometric Analysismentioning

confidence: 99%

Effect of poly (lactide‐co‐glycolide) (PLGA)‐coated beta‐tricalcium phosphate on the healing of rat calvarial bone defects: a comparative study with pure‐phase beta‐tricalcium phosphate

Bizenjima

Takeuchi

Seshima

et al. 2016

Clinical Oral Implants Res

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“…IngeniOs β-TCP Bioactive Synthetic Bone Particles (Zimmer Dental Inc., Carlsbad, CA) and OT acetate salt hydrate (Sigma-Aldrich, St. Louis, MO, USA) were purchased. OT acetate salt hydrate was reconstituted in physiological saline at a concentration of 2.5 mg OT/ml saline (16). Surgical procedure General anesthesia was induced by an intramuscular injection of a combination of 25 mg/kg body weight ketamine hydrochloride (Ketamine ® 50mg/ml) and 5mg/kg body weight Xylazine hydrochloride (Xyla-Ject ® ADWIA Co, 10th of Ramadan City, Egypt).…”

Section: Methodsmentioning

confidence: 99%

“…The osseous defects performed in each animal were washed out with sterile saline before loading. Right sided osseous defects (experimental side) were filled with Oxytocin loaded β-TCP, while left sided bone defects (control side) were filled with saline loaded β-TCP (16,17).…”

Section: Methodsmentioning

confidence: 99%

The Effect of Local Oxytocin Delivery on Healing of Bone Defects in Rabbits

Asmaa

M²,

M³

et al. 2016

Alexandria Dental Journal

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INTRODUCTION:Bone defects above critical size do not heal completely by itself and thus represent major clinical challenge to reconstructive surgery. Numerous bone substitutes have already been used to promote bone regeneration. Incorporation of 31biologically active molecules to bone grafts have shown to further improve its bone forming capacity. As a potential candidate for bone regenerating biomolecules which can be used in promoting proliferation and osteogenic differentiation of mesenchymal stem cells, the hypothalamic hormone oxytocin is suggested to be promising. AIM OF THE STUDY: was to evaluate the efficacy of the hypothalamic nonapeptide oxytocin (OT) by direct delivery into local defects using β-tricalcium phosphate (β-TCP) as a carrier. MATERIALS AND METHODS: Twenty one male New Zealand white rabbits weighing 3 Kg (±250 g) were used in this study. Right and left critical size bone defects (CSD) were performed in the edentulous area of rabbit mandibles (diastema). Right side bone defects (Experimental) were filled with OT loaded β-TCP, while the left side bone defects (Control), were filled with saline loaded β-TCP. The effect of OT hormone on bone formation was assessed histologically as well as histomorphometrically after 1, 3 and 6 weeks. RESULTS: Greater amount of new bone formation was noticed in the CSDs filled with the OT loaded groups. The amount of new bone formed was significantly higher during the 3 observational intervals in the OT loaded β-TCP than when compared with the saline loaded β-TCP. CONCLUSION: OT induces new bone formation via an osteoinductive action and can be added to bone grafts following surgical and periodontal surgeries to enhance bone regenerative capacity.

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“…With this in mind, in a first study [10] we found that bone regeneration capacity of DPSC or PeSCin SCID BEIGE nude mice, measured as a percentage of bone volume of the total defect area, did not significantly differ from that induced by the scaffolds alone when seeded with two scaffolds commercially available [GDPB [11] and (ß-TCP) [12]. Subsequently, we investigated the regeneration capacity of two previously tested biomaterials, used alone and in combination with DPSC, in nude immunodeficient rats using micro-Computed tomography (micro-CT) to evaluate Bone Mineral Density (BMD) and Positron Emission Tomography (PET) to analyze the relationship between osteoblastic and osteoclastic activities [13].…”

Section: Introductionmentioning

confidence: 99%

Histomorphometric Evaluation of Bone Regeneration Induced by Biodegradable Scaffolds as Carriers for Dental Pulp Stem Cells in a Rat Model of Calvarial "Critical Size" Defect

Annibali¹,

Quaranta²,

Scarano³

2016

J Stem Cell Res Ther

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Objective: The aim of this study was to test specific stem cells that could enhance bone formation in combination\ud with specific scaffolds.\ud Methods: Dental Pulp Stem Cells (DPSCs) were seeded with Granular Deproteinized Bovine Bone (GDPB) or Beta-Tricalcium Phosphate (ß-TCP) in a rat model of calvarial "critical size" defect. DPSCs were isolated from permanent human teeth, obtained and characterized using specific stem cells markers (Nanog and Oct-4) by real time-PCR and immunofluorescence. Cells were differentiated for 10-15 days towards the osteoblastic phenotype with 100μM L-ascorbic acid, added every day in culture medium and 20 vol. percentage of FBS in α-MEM medium. Osteogenic commitment was evaluated with real time-PCR by measuring the expression of specific markers (osteonectin and runx2). When a sufficient cell number was obtained, DPSCs were trypsinized, washed in culture medium and seeded onto the GDPB and ß-TCP scaffold sat a density of 0.5-1×106 cells/scaffold. Two bilateral critical-size circular defects (5 mm diameter; 1 mm thickness) were created from the parietal bone of the 8 athymic T-cell deficient nude rats. One cranial defect for each rat was filled with the scaffold alone and the other defect with the scaffold seeded with stem cells. After 12 weeks post-surgery animals were euthanized and histomorphometric analysis was performed. Differences between groups were analyzed by one-way analysis of variance (ANOVA) followed by Fisher's Protected Least Significant Difference (PLSD) post-hoc test. A p-value <0.05 was considered statistically significant.\ud Results: GDPB group presented higher percentage of lamellar bone than that of GDPB/DPSC, ß-TCP alone had lower levels as compared to ß-TCP/DPSC. The addition of stem cells significantly increased woven bone formation in both scaffold-based implants, although still higher in GDPB based implants.\ud Conclusion: Our findings indicate that GDPB and ß-TCP used as scaffold to induce bone regeneration may benefit from adding DPSC to tissue-engineered constructs

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Bone healing with oxytocin‐loaded microporous β‐TCPbone substitute in ectopic bone formation model and critical‐sized osseous defect of rat

Abstract: These results suggest that local OT delivery to bone substitute promotes new bone formation via an osteoinductive mode of action.

Cited by 18 publications

References 51 publications

Effect of poly (lactide‐co‐glycolide) (PLGA)‐coated beta‐tricalcium phosphate on the healing of rat calvarial bone defects: a comparative study with pure‐phase beta‐tricalcium phosphate

Effect of poly (lactide‐co‐glycolide) (PLGA)‐coated beta‐tricalcium phosphate on the healing of rat calvarial bone defects: a comparative study with pure‐phase beta‐tricalcium phosphate

The Effect of Local Oxytocin Delivery on Healing of Bone Defects in Rabbits

Histomorphometric Evaluation of Bone Regeneration Induced by Biodegradable Scaffolds as Carriers for Dental Pulp Stem Cells in a Rat Model of Calvarial "Critical Size" Defect

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