Bone histomorphometry of broilers submitted to different phosphorus sources in growing and finisher rations

Abstract

-The objective of this work was to identify alterations in the histomorphology of the cortical bone tissue of broilers submitted to growing and finisher rations formulated with five different sources of phosphorus: dicalcium phosphate, simple superphosphate, triple superphosphate, monoammonium phosphate and Araxá rock phosphate. Histological images had their components segmented, and were called regions of interest (ROI). Images were analyzed through developed algorithms, using the SCILAB mathematical environment. Eleven features were considered in order to obtain a complete description of the bone images: percentage of bone by area, ROI area, ROI perimeters, ROI elongation, ROI angle and their respective standard deviations, besides entropy of ROI angles and a texture-oriented measure (lacunarity). The substitution of dicalcium phosphate in growing and finisher rations for any other tested source of phosphorus caused significant changes on the hystomorphology of the cortical broilers bones, for example: diminution of bone percentage by area, increase of lacuna area and worse matrix homogeneity. Changes were more pronounced in the Araxá rock phosphate treatments, with the highest fluorine content, than in simple superphosphate, triple superphosphate and monoammonium phosphate treatments, which were similar.Index terms: histomorphology, macromineral sources, poultry nutrition. Histomorfometria óssea de frangos de corte submetidos a diferentes fontes de fósforo nas rações de crescimento e terminaçãoResumo -O objetivo deste trabalho foi avaliar alterações histomorfológicas na região do osso cortical de frangos de corte alimentados, nas fases de crescimento e engorda, com rações contendo cinco diferentes fontes de fósforo -fosfato dicálcico, superfosfato simples, superfosfato triplo, fosfato monoamônio e fosfato de rocha de Araxá. Imagens histológicas foram digitalizadas, segmentadas em regiões de interesse (ROI) e analisadas por meio de algoritmos desenvolvidos no ambiente de programação SCILAB. Consideraram-se onze características para descrição das imagens: porcentagem de osso por área, área da ROI, perímetro da ROI, alongamento da ROI, ângulo da ROI, os respectivos desvios-padrão dessas medidas, a entropia dos ângulos das ROI e uma medida orientada de textura (lacunaridade). A substituição do fosfato dicálcico, nas rações de crescimento e engorda, por qualquer uma das outras fontes de fósforo testadas, causou mudanças significativas na histomorfologia do córtex ósseo dos frangos de corte, tais como: menor porcentagem de osso por área, aumento da área das lacunas e menor homogeneidade da matriz. As alterações foram mais pronunciadas nos tratamentos que receberam fosfato de rocha de Araxá, fonte de maior nível de flúor, do que nos tratamentos com superfosfato simples, superfosfato triplo e fosfato monoamônio, os quais foram semelhantes entre si.Termos para indexação: histomorfologia, fontes de macrominerais, nutrição de frangos de corte.

Histological analysis of the alterations on cortical bone channels network after radiotherapy: A rabbit study

“…The 168 images captured with objective lens of 10× were segmented and analyzed using a methodology proposed by Oliveira et al (2006) (Figs. 1A–1C), which consists in an histological analysis performed by algorithms developed in the SCILAB mathematical environment, considering several morphological features of bone channels and image texture characterization.…”

Changes in cortical bone channels network and osteocyte organization after the use of zoledronic acid

“…(16) and proposed by Oliveira and cols. (17), which consists of an interactive method of drawing and selecting all channels (vascular areas as Havers and Volkmann channels) in the image ( Figure 1B). Afterward, an analysis was performed by algorithms developed in the SCILAB mathematical environment, considering several morphological features of cortical bone channels network (CBCN) and using image texture characterization.…”

Comportamiento productivo y características morfométricas y mineralización de tibias de pollos de engorde suplementados con fosfatos inorgánicos de cinco fuentes comerciales

“…Productive performance and morphometric characteristics and mineralization of tibia of broiler chickens supplemented with inorganic phosphates from five commercial sources Murga Clara T. 1 , Jenny Virhuez R. 1 , Carlos Vílchez P. 2 , Luis Nakandakari A. 1,3 Resumen…”