Bone Loss and Caries in Rats Infected with Human Streptococci

Duany, Luis F.; Zinner, Doran D.; Landy, Jerome J.

doi:10.1177/00220345710500025801

Cited by 10 publications

(2 citation statements)

References 8 publications

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“…Smooth surface caries are dependent on plaque formation [McDonald and Stookey, 1978] and S. mu tans, when cultured with sucrose forms glucans and levans which facilitate plaque formation [Mandel, 1979]. S. mutans is able to initiate fissure caries in gnotobiotic rats [Duany et al, 1971], However, the ad dition of S. mutans to the oral cavity of rats already infected with other microor ganisms does not alter the incidence of sulcal caries [Newbrun, 1977]. This was al so true for rats in our study.…”

Section: Discussionmentioning

confidence: 51%

Anticariogenicity of Dietary Glycerol Monolaurin in Rats

Lynch¹,

Schemmel²,

Kabara³

1983

Caries Res

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Compared to rats inoculated with Streptococcus mutans, non-inoculated rats did not have any buccal/lingual caries and S. mutans in the oral cavity. However, sulcal caries prevalence was similar in both groups of rats. In addition, experiments were conducted to evaluate the anticariogenic effect of glycerol monolaurin. Rats were fed the experimental diets for either 4 or 6 weeks and then killed to assess caries prevalence and microbial oral populations. Rats consuming a diet with 2% glycerol monolaurin had a range of 45–50, 14–30 and 19–22% fewer caries on buccal/lingual, proximal and sulcal surfaces, respectively, than control rats fed a diet with hydrogenated shortening. Caries prevalence was higher in mandibular than in maxillary teeth. The number of S. mutans CFUs ranged from 28 to 69% lower among rats fed glycerol monolaurin than those fed vegetable shortening.

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Section: Discussionmentioning

confidence: 51%

Anticariogenicity of Dietary Glycerol Monolaurin in Rats

Lynch¹,

Schemmel²,

Kabara³

1983

Caries Res

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“…The two enzyme pools were dialyzed extensively against distilled water and centrifuged twice at 3,000 x g for 15 min, and the supernatants were lyophilized and stored at -60°C. The specific activities of the 0.2 and 0.4 M fractions were 12 and 322 dextransucrase units (26) per mg of protein, respectively.…”

mentioning

confidence: 95%

Ultrastructural Localization of Sucrases in Streptococcus mutans GS-5 and an Extracellular Polysaccharide Mutant: a Comparative Cytochemical and Immunocytochemical Study

1977

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Electron microscopy and cytochemical and immunocytochemical procedures were used to study the ultrastructural distribution of sucrase enzymes in two strains of Streptococcus mutans. In a strongly adherent and virulent parent strain, GS-5, most of the invertase and fructosyltransferase activities were demonstrated extracellularly or bound to the cell surfaces. Intracellularly, enzymatic sites were detected near the plasma membrane on the periphery of the nucleoid and central mesosome. In GS-511, a mutant of diminished virulence and adherence, most of the enzymatic activity was not located on the cell surfaces, but was found away from the cell walls and associated with extracellular polysaccharides. Intracellularly, GS-511 manifested the same distribution of invertase and fructosyltransferase as did GS-5; however, the close association of these enzymes with the plasma membrane was not shown in GS-511. In both strains, extracellular areas near regions associated with cross wall formation appeared to show localized concentrations of these sucrases. Antibodies against partially purified glucosyltransferase (GTF) enzymes from GS-5 were used to localize GTF by immunocytochemical techniques. Indirect ferritin localization procedures showed that the extracellular and cell-bound GTF enzymes were distributed in similar locations as the fructosyltransferase and invertase enzymes. By absorption of the antiserum with whole GS-511 cells, the location of extracellular GTF and surface antigens unique to GS-5 was demonstrated. The dramatically reduced levels ofcell-bound sucrase activity in GS-511 indicates the significant role of these enzymes in adherence and cariogenicity. 447 on August 1, 2020 by guest http://iai.asm.org/ Downloaded from

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The effect of inoculating strains of Streptococcus mutans and Streptococcus sanguis upon caries incidence and bacterial content of plaque in rats

Huxley

1973

Archives of Oral Biology

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Bone Loss and Caries in Rats Infected with Human Streptococci

Cited by 10 publications

References 8 publications

Anticariogenicity of Dietary Glycerol Monolaurin in Rats

Anticariogenicity of Dietary Glycerol Monolaurin in Rats

Ultrastructural Localization of Sucrases in Streptococcus mutans GS-5 and an Extracellular Polysaccharide Mutant: a Comparative Cytochemical and Immunocytochemical Study

The effect of inoculating strains of Streptococcus mutans and Streptococcus sanguis upon caries incidence and bacterial content of plaque in rats

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