Bone markers and osteoporosis therapy

Bandeira, Francisco; Costa, Aline G.; Filho, Manoel Aderson Soares; Pimentel, Larissa; Lima, Lourena; Bilezikian, John P.

doi:10.1590/0004-2730000003384

Cited by 25 publications

(16 citation statements)

References 54 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The transcription factor Runx2 also has been found negatively regulated by miR-204 and miR-211 to inhibit osteogenesis by binding to Runx2 3′UTR [42]. Osteocalcin, as a key marker of osteoblast activity and bone formation, can not only affect osteoid mineralization but also play a part in negative feedback during the process of bone remodeling [43,44]. Positive osteocalcin exists in malignant osteoblasts in all cases of OS [45].…”

Section: Discussionmentioning

confidence: 99%

Up-regulation of microRNA-340 promotes osteosarcoma cell apoptosis while suppressing proliferation, migration, and invasion by inactivating the CTNNB1-mediated Notch signaling pathway

Pan

et al. 2018

Bioscience Reports

View full text Add to dashboard Cite

Osteosarcoma (OS) is the most common histological form of primary bone cancer. It is most prevalent in teenagers and young adults. The present study aims at exploring the regulatory effect of microRNA-340 (miR-340) on OS cell proliferation, invasion, migration, and apoptosis via regulating the Notch signaling pathway by targeting β-catenin (cadherin-associated protein) 1 (CTNNB1). OS tissues belonging to 45 patients and normal femoral head tissues of 45 amputees were selected. Cells were allocated to different groups. In situ hybridization was performed to determine the positive rate of miR-340 expression while immunohistochemistry was used to determine that of CTNNB1 and B-cell lymphoma 2 (Bcl-2). We used a series of experiments to measure the expressions of related factors and assess rates of cell proliferation, migration, invasion, cycle, and apoptosis respectively. Our results show that miR-340 was expressed a higher level in normal tissue than OS tissue. Expression of Notch, CTNNB1, hairy and enhancer of split 1 (Hes1), Bcl-2, Runt-related transcription factor 2 (Runx2), and osteocalcin increased and that of miR-340, Bcl-2 interacting mediator of cell death (BIM), and Bcl-2 associated protein X (Bax) decreased in OS tissues. U-2OS cell line had the highest miR-340 expression. We also found that the up-regulation of miR-340 had increased expression of miR-340, BIM, and Bax but decreased expression of Notch, CTNNB1, Hes1, Bcl-2, Runx2, and osteocalcin. Up-regulation of miR-340p lead to increased cell apoptosis, suppressed cell proliferation, migration, and invasion. Our study demonstrates that overexpression of miR-340 could suppress OS cell proliferation, migration, and invasion as well as promoting OS cell apoptosis by inactivating the Notch signaling pathway via down-regulating CTNNB1. Functional miR-340 overexpression might be a future therapeutic strategy for OS.

show abstract

Section: Discussionmentioning

confidence: 99%

Up-regulation of microRNA-340 promotes osteosarcoma cell apoptosis while suppressing proliferation, migration, and invasion by inactivating the CTNNB1-mediated Notch signaling pathway

Pan

et al. 2018

Bioscience Reports

View full text Add to dashboard Cite

show abstract

“…Vitamin D is called the antirachitic vitamin, since it counteracts rickets which cause breaking‐down mineral substance in fully grown bones (Osteodistrophy), bone and joint deformations, and spontaneous brittleness of bones . The term vitamin D refers to a pair of biologically inactive precursors: Vitamin D 3 known as cholecalciferol (VD 3 ) and vitamin D 2 known as ergocalciferol (VD 2 ).…”

Section: Introductionmentioning

confidence: 99%

Encapsulation of cholecalciferol and ergocalciferol in oil‐in‐water emulsions by different homogenization techniques

Khalid

Kobayashi

Neves

et al. 2016

Euro J Lipid Sci & Tech

View full text Add to dashboard Cite

Ergocalciferol (VD2) and cholecalciferol (VD3) are bioactive compounds with reduced bioavailability. Soybean oil, olive oil, or medium chain triglyceride containing 0.5% w/w VD2 and VD3, was used as a dispersed phase, while phosphate buffer containing 1% w/w Tween 20 served as a continuous phase. The two phases were emulsified with a rotor‐stator homogenizer (RSH) at 5000–20 000 rpm for 5 min or with high pressure homogenization (HPH) at 100 MPa after RSH at 7000 rpm for 5 min. The volume mean diameter (d4,3) of the formulated oil‐in‐water (O/W) emulsions gradually decreased with increasing the rotation speed of RSH. The O/W emulsions formulated under appropriate homogenization conditions remained stable over 30 days of storage period. There was no prominent difference in d4,3 of the O/W emulsions formulated with the HPH, differing from d4,3 of the O/W emulsions formulated with the RSH during 30 days of storage period. There was little effect of homogenization types on release profile of the VD2 and VD3 loaded emulsions. The encapsulation efficiencies of VD2 and VD3 were less than 70% after 10 days of storage, followed by sharp decline to 10% after 30 days at 4°C. Practical applications: Multiple studies have emphasized the benefits to human health of including vitamins in the diet and as special nutrient formula for athletes. For this reason, our study was proposed to formulate a stable emulsion system that could later be added to formulate high energy food or energy drinks. A mixture of cholecalciferol (VD2) and ergocalciferol (VD3) is successfully encapsulated in the O/W emulsions. The different homogenization conditions have no significant effect on the encapsulation efficiencies. There is sharp decline in encapsulation efficiencies of VD2 and VD3 after 15 days of storage.

show abstract

“…Drugs being developed or used for treating osteoporosis include estrogen replacement therapy, calcitonin, selective estrogen receptor modulators, and diphosphate. Although these drugs prevent bone resorption, their effects on bone formation are extremely small (Abrahamsen et al, 2014;Bandeira et al, 2014;Chen and Kubo, 2014;Kulak et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

Intestinal Absorption and Metabolism of Epimedium Flavonoids in Osteoporosis Rats

et al. 2015

View full text Add to dashboard Cite

Herba Epimdii is a traditional Chinese medicine used to treat osteoporosis. Its main pharmacological ingredients are flavonoids. In previous studies conducted in healthy animals, we showed that epimedium flavonoids could be hydrolyzed into secondary glycosides or aglycon by intestinal flora or enzymes, thereby enhancing their absorption and antiosteoporosis activity. To study the medicine in the pathologic state, epimedium flavonoids were incubated with intestinal mucosa and feces in vitro and intestinal perfusion in situ to explore the differences in absorption and metabolism between sham and osteoporosis rats. For osteoporosis rats, the hydrolysis rates of icariin, epimedin A, epimedin B, and epimedin C incubated with intestinal flora for 1 hour were reduced by 0.19, 0.26, 0.19, and 0.14, respectively, compared with that in sham rats. Hydrolysis rates were reduced by 0.21, 0.24, 0.08, and 0.31 for icariin, epimedin A, epimedin B, and epimedin C incubated with duodenal enzymes for 1 hour and by 0.13, 0.09, 0.07, and 0.47 for icariin, epimedin A, epimedin B, and epimedin C incubated with jejunum enzymes, respectively, compared with the sham group. In addition, the apparent permeability coefficient and elimination percentage of the four epimedium flavonoids in the duodenum, jejunum, ileum, and colon decreased by 29%-44%, 32%-50%, 40%-56%, and 27%-53% compared with that in sham rats, respectively. The main metabolites of the four epimedium flavonoids were the same for the two groups after intestinal perfusion, or flora and enzyme incubation. In conclusion, the amount and activity of intestinal flora and enzymes changed in ovariectomized rats, which affected the intestinal absorption and hydrolysis of epimedium flavonoids whose structures contain 7-glucose.

show abstract

Bone markers and osteoporosis therapy

Cited by 25 publications

References 54 publications

Up-regulation of microRNA-340 promotes osteosarcoma cell apoptosis while suppressing proliferation, migration, and invasion by inactivating the CTNNB1-mediated Notch signaling pathway

Up-regulation of microRNA-340 promotes osteosarcoma cell apoptosis while suppressing proliferation, migration, and invasion by inactivating the CTNNB1-mediated Notch signaling pathway

Encapsulation of cholecalciferol and ergocalciferol in oil‐in‐water emulsions by different homogenization techniques

Intestinal Absorption and Metabolism of Epimedium Flavonoids in Osteoporosis Rats

Contact Info

Product

Resources

About