Bone Marrow Biopsy for the Staging of Non-Hodgkin's Lymphoma: Bilateral or Unilateral Trephine Biopsy?

Luoni, M; Declich, Paolo; p, De Paoli Af; Fava, Sergio; Marinoni, P; Montalbetti, L; Sangalli, Gabriele; Sciuccati, P; Tocci, Angelo; Tosi, Anna

doi:10.1177/030089169508100604

Cited by 15 publications

(12 citation statements)

References 8 publications

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“…This is supported by the improved sensitivity of bilateral over unilateral BMB. 22 Furthermore, dilution of the aspirate samples with peripheral blood during the procedure may decrease the proportion of neoplastic cells to below the threshold of detection by FC.…”

Section: Discussionmentioning

confidence: 99%

A comparison of flow cytometry, bone marrow biopsy, and bone marrow aspirates in the detection of lymphoid infiltration in B cell disorders

Sah

Matutes²,

Wotherspoon³

et al. 2003

Journal of Clinical Pathology

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Aims: To evaluate the diagnostic value of bone marrow aspirates, trephine biopsies (BMB), and flow cytometry (FC) in the assessment of bone marrow infiltration in chronic lymphoid disorders. Methods: Investigations were carried out in 110 diagnostic and follow up specimens from B cell disorders, namely: chronic lymphocytic leukaemia (CLL; 65), non-Hodgkin's lymphoma (NHL; 39), and hairy cell leukaemia (HCL; 6). A selected panel of monoclonal antibodies was used both for FC and immunohistochemistry. Results: In CLL there was agreement between the three investigations in 71% of samples and in 88% when only FC and BMB were compared. In nine of 65 samples, FC and BMB were positive, although the aspirate was reported as negative. Four BMB negative samples had minimal residual disease (MRD) detected by FC, whereas two samples were positive both on BMB and aspirate but showed no evidence of disease on FC. In NHL, there was agreement between the three investigations in 22 of 39 cases, and in 27 of 39 cases there was agreement between FC and BMB. In eight of 39 NHL cases, FC was negative but the BMB was either positive (five) or uncertain (three), whereas in three of 39, FC was positive but BMB was either negative (one) or uncertain (two). In three of five uncertain BMB, no clonal population was detected by the polymerase chain reaction, whereas in the remaining two cases the nodular aggregates disappeared on further sectioning. Conclusions: Both BMB and FC are better than bone marrow aspirates for the detection of infiltration in B cell disorders. FC might be slightly more sensitive than BMB to detect MRD in CLL, whereas BMB may be slightly better than FC in NHL.

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Section: Discussionmentioning

confidence: 99%

A comparison of flow cytometry, bone marrow biopsy, and bone marrow aspirates in the detection of lymphoid infiltration in B cell disorders

Sah

Matutes²,

Wotherspoon³

et al. 2003

Journal of Clinical Pathology

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“…The trephine biopsy specimen should be at least 2 cm and mostly comprised of trabecular bone (70). Bilateral bone marrow samples should be obtained if possible, since many studies have indicated a high higher incidence of detection than with unilateral specimens (74)(75)(76)(77)(78)(79). A bone marrow aspirate and biopsy touch imprint should be obtained if possible, since these specimens may preempt the biopsy findings and guide the use of special studies such as flow cytometry and fluorescence-in-situ hybridization (80).…”

Section: Nonhematopoietic Tumorsmentioning

confidence: 99%

Bone marrow aspirate and biopsy: a pathologist's perspective. II. interpretation of the bone marrow aspirate and biopsy

Riley

Williams

Ross

et al. 2009

Clinical Laboratory Analysis

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Bone marrow examination has become increasingly important for the diagnosis and treatment of hematologic and other illnesses. Morphologic evaluation of the bone marrow aspirate and biopsy has recently been supplemented by increasingly sophisticated ancillary assays, including immunocytochemistry, cytogenetic analysis, flow cytometry, and molecular assays. With our rapidly expanding knowledge of the clinical and biologic diversity of leukemia and other hematologic neoplasms, and an increasing variety of therapeutic options, the bone marrow examination has became more critical for therapeutic monitoring and planning optimal therapy. Sensitive molecular techniques, in vitro drug sensitivity testing, and a number of other special assays are available to provide valuable data to assist these endeavors. Fortunately, improvements in bone marrow aspirate and needle technology has made the procurement of adequate specimens more reliable and efficient, while the use of conscious sedation has improved patient comfort. The procurement of bone marrow specimens was reviewed in the first part of this series. This paper specifically addresses the diagnostic interpretation of bone marrow specimens and the use of ancillary techniques.

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“…Despite conflicting evidence to support its efficacy as an ancillary tool in initial diagnosis and staging [11,[16][17][18][19][20], FC is frequently requested concurrent with LM examination of tissue or cellular morphology. It is well established that increasing the number of specimens submitted for morphologic assessment increases sensitivity of detection of malignant diseases [21][22][23][24].…”

Section: Discussionmentioning

confidence: 99%

A quality improvement assessment of multiple, concurrent flow cytometry analyses at a tertiary care center

Karnes

Frater

2014

Int J Lab Hematology

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Bone Marrow Biopsy for the Staging of Non-Hodgkin's Lymphoma: Bilateral or Unilateral Trephine Biopsy?

Cited by 15 publications

References 8 publications

A comparison of flow cytometry, bone marrow biopsy, and bone marrow aspirates in the detection of lymphoid infiltration in B cell disorders

A comparison of flow cytometry, bone marrow biopsy, and bone marrow aspirates in the detection of lymphoid infiltration in B cell disorders

Bone marrow aspirate and biopsy: a pathologist's perspective. II. interpretation of the bone marrow aspirate and biopsy

A quality improvement assessment of multiple, concurrent flow cytometry analyses at a tertiary care center

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