BONE MARROW BLOOD VOLUME AND TOTAL RED CELL MASS OF THE GUINEA‐PIG AS DETERMINED BY ⁵⁹Fe‐ERYTHROCYTE DILUTION AND LIQUID NITROGEN FREEZING

Abstract

The blood content and circulating red cell volumes of bone marrow and lymphoid tissues have been determined by means of a 59Fe‐labelled erythrocyte technique coupled with liquid nitrogen freezing in male guinea‐pigs. The marrow blood volume represented 4·90–6·96 per cent of the total marrow volume and showed regional differences which have been partially correlated with marrow cellularity. It has been calculated that the entire bone marrow of the guinea‐pig normally contains 1·7 per cent of the whole body blood volume and 2·0 per cent of the whole body circulating red cell mass. At 3 hr. after the injection of labelled erythrocytes there was evidence of a selective pooling in the spleen and bone marrow of recipient animals. The whole body blood volume was calculated to be 5·91 ml./100 g. body weight and the ratio of body hæmatocrit to cardiac hæmatocrit was 0·846. A comparison of the numbers of circulating erythrocytes in marrow (isotope dilution technique) with total erythrocyte numbers determined in suspensions of disrupted marrow was in accord with the view that the non‐nucleated, non‐reticulated marrow erythrocytes are intravascular.

Disposition and exposure of the fibrinogen receptor antagonist XV459 on αIIBβ3 binding sites in the guinea pig

“…Table 5 contains the actual recovery results shown as the percentage of 3 H recovered in the selected tissues relative to the administered dose in individual guinea pigs. Recovery in blood and bone marrow was based on total blood volume in the guinea pig being 6.96% of total body weight [25] and that total skeletal bone marrow was approximately 7.014 mL for a 350-450 g guinea pig [26]. Based on the recovery of the two animals sacrificed at the 72-h point, more than 75% of the administered dose was recovered in the urine and the faeces by 72 h, with the primary ( 66%) elimination pathway being urinary excretion.…”

“…Understanding these phenomena permits the selection of more relevant animal species to explore PK/PD relationships and to ultimately make inferences on human disposition of these agents. While the rhesus monkey [25] and most probably higher primate species share similar receptor expression and affinities with man, the guinea pig appears to be the most relevant rodent species for PK/PD with this class of agents.…”

Identity of Erythroblast Precursors in Bone Marrow

“…The total erythroid output by the marrow may be determined from the knowledge of (a) the bone marrow [16] and blood volume [17], (b) the daily physiological loss in red cells [18], (c) the increase in the red cell count [8], (d) the number of marrow reticulocytes [8], and (e) the number of erythro-blasts. During the first 3 days following bleeding, 7 X 10* cells were produced by the marrow, splenic red cell output at this time being negligible.…”

Hematological Assessment in Pet Guinea Pigs (Cavia porcellus)

“…4,8,14 That range is based on the consensus that 7% to 10% of blood volume can safely be collected at any 1 time, with an average adult blood volume of 69 to 75 mL/kg of body weight. 4,15,16 …”