Bone marrow cellular composition in Listeria monocytogenes infected mice detected using ER-MP12 and ER-MP20 antibodies: a flow cytometric alternative to differential counting

Bruijn, Marella F. T. R. de; Vianen, Wim van; Ploemacher, R. E.; Bakker-Woudenberg, Irma A. J. M.; Campbell, Priscilla A.; Ewijk, Willem van; Leenen, Pieter J. M.

doi:10.1016/s0022-1759(98)00080-5

Cited by 42 publications

(50 citation statements)

References 33 publications

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“…When infected leg bones were excised and imaged, the bones exhibited focal signals indicating that the infection was not present in soft tissues. The bacteria infecting the legs were located in the bone marrow, as determined by the recovery of CFU from that compartment, which is consistent with previous studies (de Bruijn et al, 1998;Join-Lambert et al, 2005). However, no granulomatous lesions or other indications of inflammation were discernable in the infected bones by histology.…”

Section: Introductionsupporting

confidence: 90%

“…monocytogenes can be cultured from the bone marrow of infected mice (de Bruijn et al, 1998) and has been shown to infect myeloid cells of the surface phenotype: CD31 + , Ly-6C + , CD11b + (Join-Lambert et al, 2005). These cells are found in the bone marrow and the blood, and represent an important reservoir of the pathogen with regard to the infection of the central nervous system.…”

Section: Introductionmentioning

confidence: 99%

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Foci of Listeria monocytogenes persist in the bone marrow

Hardy

Chu²,

Contag

2009

Disease Models &Amp; Mechanisms

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SUMMARYMurine listeriosis is one of the most comprehensive and well-studied models of infection, and Listeria monocytogenes has provided seminal information regarding bacterial pathogenesis. However, many aspects of the mouse model remain poorly understood, including carrier states and chronic colonization which represent important features of the spectrum of host-pathogen interaction. Bone marrow has recently been shown to harbor L. monocytogenes, which spreads from this location to the central nervous system. Bone could, therefore, be an important chronic reservoir, but this infection is difficult to study because it involves only a few bacteria and the extent of infection cannot be assessed until after the animal is sacrificed. We employed in vivo bioluminescence imaging to localize L. monocytogenes bone infections over time in live mice, revealing that the bacteria grow in discrete foci. These lesions can persist in many locations in the legs of mice and are not accompanied by a histological indication such as granuloma or a neutrophil infiltratate. We demonstrate that highly attenuated hly mutants, which have defective intracellular replication, are capable of prolonged focal infection of the bone marrow for periods of up to several weeks. These results support the recently proposed hypothesis that the bone marrow is a unique niche for L. monocytogenes.

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Section: Introductionsupporting

confidence: 90%

Section: Introductionmentioning

confidence: 99%

Foci of Listeria monocytogenes persist in the bone marrow

Hardy

Chu²,

Contag

2009

Disease Models &Amp; Mechanisms

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“…In addition, at least a fraction has the potential to develop into DC (6 -8). Monocytes can be identified in the BM on the basis of high level expression of Ly-6C (ER-MP20) and the absence of CD31 (ER-MP12), but an exclusive marker for mouse monocytes in the BM or the bloodstream is not yet available (4,9,10). Characterizing peripheral blood monocytes, Lagasse and Weissman (11) and, more recently, Henderson et al (12) showed peripheral blood monocytes to be a homogeneous population.…”

mentioning

confidence: 99%

Subpopulations of Mouse Blood Monocytes Differ in Maturation Stage and Inflammatory Response

Sunderkötter

Nikolić

Dillon

et al. 2004

The Journal of Immunology

1,002

1,097

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Blood monocytes are well-characterized precursors for macrophages and dendritic cells. Subsets of human monocytes with differential representation in various disease states are well known. In contrast, mouse monocyte subsets have been characterized minimally. In this study we identify three subpopulations of mouse monocytes that can be distinguished by differential expression of Ly-6C, CD43, CD11c, MBR, and CD62L. The subsets share the characteristics of extensive phagocytosis, similar expression of M-CSF receptor (CD115), and development into macrophages upon M-CSF stimulation. By eliminating blood monocytes with dichloromethylene-bisphosphonate-loaded liposomes and monitoring their repopulation, we showed a developmental relationship between the subsets. Monocytes were maximally depleted 18 h after liposome application and subsequently reappeared in the circulation. These cells were exclusively of the Ly-6Chigh subset, resembling bone marrow monocytes. Serial flow cytometric analyses of newly released Ly-6Chigh monocytes showed that Ly-6C expression on these cells was down-regulated while in circulation. Under inflammatory conditions elicited either by acute infection with Listeria monocytogenes or chronic infection with Leishmania major, there was a significant increase in immature Ly-6Chigh monocytes, resembling the inflammatory left shift of granulocytes. In addition, acute peritoneal inflammation recruited preferentially Ly-6Cmed-high monocytes. Taken together, these data identify distinct subpopulations of mouse blood monocytes that differ in maturation stage and capacity to become recruited to inflammatory sites.

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“…[29][30][31] The aspects of L. monocytogenes which provide a rationale for the design of potent, specific and safe vaccine platforms are (1) Listeria can be easily grown under standard BSL2 laboratory conditions in an animal product free medium, (2) techniques for genetic manipulation of this organism are well-established allowing for the construction of attenuated recombinant vaccine strains that are safe for human use, (3) it is easily manipulated to express multiple gene products using plasmid or chromosomal systems, (4) there is extensive knowledge about its life cycle, genetics and immunological characteristics and (5) it directly infects and grows intracellularly in antigen-presenting cells (APC) such as monocytes, macrophages and dendritic cells (DC), thereby delivering the TAA into their cytoplasm, resulting in processing and presentation of the antigen by the immune system. These characteristics make the use of L. monocytogenes favorable as vaccine delivery vector to deliver not only antigenic proteins but also other molecular agents such as DNA and RNA.…”

Section: O N O T D I S T R I B U T Ementioning

confidence: 99%