Bone Marrow-Derived Mesenchymal Stem Cells Influence Early Tendon-Healing in a Rabbit Achilles Tendon Model

Chong, Alphonsus K.S.; Ang, Abel Damien; Goh, James Cho Hong; Hui, James; Lim, Aymeric; Lee, Eng Hin; Lim, Beng Hai

doi:10.2106/jbjs.e.01396

Cited by 281 publications

(197 citation statements)

References 25 publications

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“…2,8,27,61 BMSCs contracted onto pretensioned suture resulted in greater loadrelated structural and material properties when used to repair Achilles tendon defects. 61 Similar improvements were seen when a BMSC-collagen mixture was implanted into surgically created patellar tendon defects.…”

Section: Stem Cell Augmentation Of Tendon Healing and Rc Repairmentioning

confidence: 99%

“…The authors conclude that improvements in primary tendon repair with intratendinous cell therapy are restricted to the early stages of tendon healing. 8 Two different studies recently used a rat Achilles tendon model to compare MSC therapy versus other cell therapy, with important results. In one report, the Achilles was transected, including the enthesis, before surgical repair without cell injection, with chondrocyte injection, or with MSC injection.…”

Section: Stem Cell Augmentation Of Tendon Healing and Rc Repairmentioning

confidence: 99%

See 1 more Smart Citation

Biologic approaches to enhance rotator cuff healing after injury

Isaac

Gharaibeh

Witt

et al. 2012

Journal of Shoulder and Elbow Surgery

119

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Section: Stem Cell Augmentation Of Tendon Healing and Rc Repairmentioning

confidence: 99%

Section: Stem Cell Augmentation Of Tendon Healing and Rc Repairmentioning

confidence: 99%

Biologic approaches to enhance rotator cuff healing after injury

Isaac

Gharaibeh

Witt

et al. 2012

Journal of Shoulder and Elbow Surgery

119

View full text Add to dashboard Cite

“…A wide range of donor cell types have been used to promote tendon healing in pre-clinical studies, including bone marrow-derived mesenchymal stem cells (BMSCs) [3,4,5], periodontal ligament or gingival tissue [6], dermal fibroblast [7], tenocytes [8], peripheral blood-derived MSCs [9,10] and embryonic stem cell derived MSCs (ESC-MSCs) [11]. Each cell source has its limitations including inferior proliferation, differentiation and collagen production abilities of tenocytes [12,13,14,15], risk of teratoma formation of ESCs as well as risk of ectopic bone [3] and tumor induction [16] of BMSCs when using a certain scaffold.…”

Section: Introductionmentioning

confidence: 99%

Tendon Derived Stem Cells Promote Platelet-Rich Plasma Healing in Collagenase-Induced Rat Achilles Tendinopathy

Chen

Liu

Tang

et al. 2014

Cell Physiol Biochem

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Background/Aims: Tendon injuries are common, difficult to cure and usually healed with fibrosis and scar tissue. The aim of this study was to evaluate tendon derived stem cells (TDSCs) and platelet rich plasma (PRP) in the treatment of collagenase induced Achilles tendinopathy in rat. Methods: Four and 8 weeks (n=18) after TDSCs, PRP, PRP with TDSC or PBS (control) injection into collagenase or saline (sham) injected rat Achilles tendon, tendon tissue was harvested and tendon quality was evaluated by histology and biomechanical testing. TDSCs were cultured and treated by 10% PRP, and the FAK/ERK1/2 signaling pathway and tenocyte-related genes were detected by western blot analysis. Results: Compared to the control, PRP treatment resulted in better healing of injured tendons with improved histological outcomes and biomechanical functions. The addition of TDSCs to PRP treatment significantly enhanced the effects of PRP treatment alone. TDSC injection alone had little effect on tendon healing. PRP and PRP with TDSC treatments of collagenase induced tendon injuries also increased the mRNA and protein expression of tenocyte-related genes (type I collagen, SCX, Tenascin C) and activated the focal adhesion kinase (FAK) and extracellular-regulated kinase (ERK) 1/2 signaling pathways. Treatment of TDSCs in vitro with 10% PRP significantly increased the phosphorylation levels of FAK and ERK1/2 and the protein levels of tenocyte-related genes (Col I, SCX and Tenascin C). Inhibition of the FAK and ERK1/2 signaling pathways abolished the effect of PRP. Conclusion: This study concludes that PRP combined with TDSCs is potentially effective for the treatment of tendinopathy. The PRP induced, FAK and ERK1/2 dependent activation of tenocyte related genes in TDSCs in vitro suggests that the beneficial healing effect of the PRP with TDSC combination might occur by means of an improved TDSC differentiation toward the tenocyte lineage. Thus, a PRP with TDSC combination therapy may be clinically useful.

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“…Experimental laboratory studies on animal models using MSCs embedded on various types of scaffolds have returned some encouraging but inhomogenous results. Chong et al 40 used MSCs with fibrin sealant in a rabbit Achilles tendon model. In their study, no differences between fibrin and fibrin with MSC could be shown histologically.…”

Section: Resultsmentioning

confidence: 99%

Application of stem cells in orthopaedic conditions: what is the current evidence?

Tripathy¹,

Behera²,

Sen³

et al. 2013

OA Orthopaedics

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Bone Marrow-Derived Mesenchymal Stem Cells Influence Early Tendon-Healing in a Rabbit Achilles Tendon Model

Abstract: Intratendinous cell therapy with bone marrow-derived mesenchymal stem cells following primary tendon repair can improve histological and biomechanical parameters in the early stages of tendon-healing.

Cited by 281 publications

References 25 publications

Biologic approaches to enhance rotator cuff healing after injury

Biologic approaches to enhance rotator cuff healing after injury

Tendon Derived Stem Cells Promote Platelet-Rich Plasma Healing in Collagenase-Induced Rat Achilles Tendinopathy

Application of stem cells in orthopaedic conditions: what is the current evidence?

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