Bone Marrow-Derived Mesenchymal Stem Cells Enhance Cryopreserved Trachea Allograft Epithelium Regeneration and Vascular Endothelial Growth Factor Expression

Ni, Lan; ChaoJian, Pang; Tong, Xin‐Kang

doi:10.1097/tp.0b013e31822a4082

Cited by 12 publications

(12 citation statements)

References 47 publications

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“…Bone-marrow-derived mesenchymal stem cells (BMSCs) have been reported to be able to treat numerous types of disease in animals, including acute kidney failure (8), ischemic heart disease (9), lung injury (10) and intracerebral hemorrhage (11) tissues in response to damage, including necrosis (12), hypoxia (13) and ischemia (14). The mechanisms by which BMSCs alleviate acute liver injury involve challenges.…”

Section: Introductionmentioning

confidence: 99%

Bone marrow-derived mesenchymal stem cells attenuate acute liver injury and regulate the expression of fibrinogen-like-protein 1 and signal transducer and activator of transcription 3

Zou

Cai

Chen

et al. 2015

Molecular Medicine Reports

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Abstract. In recent years, bone marrow-derived mesenchymal stem cells (BMSCs) have been demonstrated to exert extensive therapeutic effects on acute liver injury; however, the underlying mechanisms of these effects have remained to be elucidated. The present study focused on the potential anti-apoptotic and pro-regenerative effects of BMSCs in D-galactosamine (D-Gal) and lipopolysaccharide (LPS)-induced acute liver injury in rats. An experimental rat acute liver injury model was established by intraperitoneal injection of D-Gal (400 mg/kg) and LPS (80 µg/kg). BMSCs and an identical volume of saline were administered via the caudal vein 2 h after the D-Gal and LPS challenge. Subsequently, the serum samples were collected to detect the levels of alanine aminotransferase and aspartate aminotransferase. Hematoxylin and eosin staining, terminal deoxynucleotidyl transferase-mediated nick-end labeling assay and immunohistochemical staining were performed to determine apoptosis, regeneration and histological changes of liver sections. Western blotting and reverse transcription-quantitative polymerase chain reaction were performed to detect the protein and mRNA expression levels of fibrinogen-like-protein 1 (FGL1), phosphorylated signal transducer and activator of transcription 3 (p-STAT3), STAT3 and B-cell lymphoma 2 (Bcl-2) and Bcl-2 associated X protein (Bax) in liver tissue samples. The results indicated that intravenous transplantation of BMSCs significantly decreased the levels of alanine aminotransferase and aspartate aminotransferase, and reduced hepatocellular necrosis and inflammatory cell infiltration. Additionally, a terminal deoxynucleotidyl transferase-mediated nick-end labeling assay and immunohistochemical staining revealed that BMSC treatment reduced hepatocyte apoptosis and enhanced liver regeneration. Furthermore, Bcl-2 expression was increased, whilst the protein expression of Bax was reduced. The expression of FGL1 and p-STAT3 were elevated concurrently with the improvement of liver function. These results demonstrated that BMSCs may provide a promising potential agent for the prevention of acute liver injury via inhibition of hepatocyte apoptosis and acceleration of liver regeneration. The mechanism may be, a least in part, a consequence of the upregulation of FGL1 expression and the induction of STAT3 phosphorylation.

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Section: Introductionmentioning

confidence: 99%

Bone marrow-derived mesenchymal stem cells attenuate acute liver injury and regulate the expression of fibrinogen-like-protein 1 and signal transducer and activator of transcription 3

Zou

Cai

Chen

et al. 2015

Molecular Medicine Reports

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“…Several studies showed that bMSC promotes neo-angiogenesis. Han et al demonstrated that bMSC may enhance neo-vascularization in cryopreserved trachea allograft by upregulating vascular endothelial growth factor expression [ 21 ]. Hence, in our study, the abundant neo-vascularization was associated with the angiogenic potential of bMSC.…”

Section: Discussionmentioning

confidence: 99%

3D Bioprinted Artificial Trachea with Epithelial Cells and Chondrogenic-Differentiated Bone Marrow-Derived Mesenchymal Stem Cells

Bae

Lee

Park

et al. 2018

IJMS

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Tracheal resection has limited applicability. Although various tracheal replacement strategies were performed using artificial prosthesis, synthetic stents and tissue transplantation, the best method in tracheal reconstruction remains to be identified. Recent advances in tissue engineering enabled 3D bioprinting using various biocompatible materials including living cells, thereby making the product clinically applicable. Moreover, clinical interest in mesenchymal stem cell has dramatically increased. Here, rabbit bone marrow-derived mesenchymal stem cells (bMSC) and rabbit respiratory epithelial cells were cultured. The chondrogenic differentiation level of bMSC cultured in regular media (MSC) and that in chondrogenic media (d-MSC) were compared. Dual cell-containing artificial trachea were manufactured using a 3D bioprinting method with epithelial cells and undifferentiated bMSC (MSC group, n = 6) or with epithelial cells and chondrogenic-differentiated bMSC (d-MSC group, n = 6). d-MSC showed a relatively higher level of glycosaminoglycan (GAG) accumulation and chondrogenic marker gene expression than MSC in vitro. Neo-epithelialization and neo-vascularization were observed in all groups in vivo but neo-cartilage formation was only noted in d-MSC. The epithelial cells in the 3D bioprinted artificial trachea were effective in respiratory epithelium regeneration. Chondrogenic-differentiated bMSC had more neo-cartilage formation potential in a short period. Nevertheless, the cartilage formation was observed only in a localized area.

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“…Previously, several studies have shown that BMSCs can be isolated from various sources and are characterized by their ability to proliferate in culture, as well as their potential to give rise to cells of multiple lineages through their self-renewal, differential properties [94][95][96][97]. Promising results have been obtained after application of BMSCs for treatment of the tracheal defects [58,59,98]. These studies demonstrated that after transplantation with allografts, BMSCs could migrate to the defected areas and promote regeneration of the tracheal epithelium and revascularization of tracheal implants, thus finally promoting functional restoration to normal tracheal epithelium.…”

Section: Bone Marrow Mesenchymal Stem Cellsmentioning

confidence: 98%

Re-Epithelialization: A Key Element in Tracheal Tissue Engineering

Zhang

2015

Regen. Med.

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Trachea-tissue engineering is a thriving new field in regenerative medicine that is reaching maturity and yielding numerous promising results. In view of the crucial role that the epithelium plays in the trachea, re-epithelialization of tracheal substitutes has gradually emerged as the focus of studies in tissue-engineered trachea. Recent progress in our understanding of stem cell biology, growth factor interactions and transplantation immunobiology offer the prospect of optimization of a tissue-engineered tracheal epithelium. In addition, advances in cell culture technology and successful applications of clinical transplantation are opening up new avenues for the construction of a tissue-engineered tracheal epithelium. Therefore, this review summarizes current advances, unresolved obstacles and future directions in the reconstruction of a tissue-engineered tracheal epithelium.

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Bone Marrow-Derived Mesenchymal Stem Cells Enhance Cryopreserved Trachea Allograft Epithelium Regeneration and Vascular Endothelial Growth Factor Expression

Abstract: Our results indicate that given systemic administration, BMSCs may enhance epithelium regeneration and revascularization by upregulating VEGF expression.

Cited by 12 publications

References 47 publications

Bone marrow-derived mesenchymal stem cells attenuate acute liver injury and regulate the expression of fibrinogen-like-protein 1 and signal transducer and activator of transcription 3

Bone marrow-derived mesenchymal stem cells attenuate acute liver injury and regulate the expression of fibrinogen-like-protein 1 and signal transducer and activator of transcription 3

3D Bioprinted Artificial Trachea with Epithelial Cells and Chondrogenic-Differentiated Bone Marrow-Derived Mesenchymal Stem Cells

Re-Epithelialization: A Key Element in Tracheal Tissue Engineering

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