Bone Marrow-Derived Mesenchymal Stromal Cells Enhanced by Platelet-Rich Plasma Maintain Adhesion to Scaffolds in Arthroscopic Simulation

Hoberman, Alexander; Cirino, Carl M.; McCarthy, Mary Beth; Cote, Mark P.; Pauzenberger, Leo; Beitzel, Knut; Mazzocca, Augustus D.; Dyrna, Felix

doi:10.1016/j.arthro.2017.08.291

Cited by 23 publications

(30 citation statements)

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“…Lots of basic science studies on cell cultures and animal models as well as human clinical trials supported the application of PRP. [26][27][28] Numbers studies have focused on its effect on multiple stem cells proliferation, [29][30][31][32] EnMSCs could be conveniently segregated from menstrual blood and expanded to create almost unlimited supplies. There are several advantages of EnMSCs, including embryoniclike properties, noninvasive isolation procedures, and improved function.…”

Section: Discussionmentioning

confidence: 99%

Investigation of platelet‐rich plasma in increasing proliferation and migration of endometrial mesenchymal stem cells and improving pregnancy outcome of patients with thin endometrium

Wang

Liu

Mou

et al. 2018

J of Cellular Biochemistry

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Background Platelet‐rich plasma (PRP) contains abundant growth factors and is gradually used in the field of reproduction. A thin endometrium is recognized as a critical factor in embryo implantation failure. Endometrial mesenchymal stem cells (EnMSCs), which were isolated from human menstrual blood, are highly proliferative and show multiple differentiation capacity. The current study was to investigate the effect of PRP on the proliferation and migration of EnMSCs, and the effectiveness of PRP in the treatment of patients with thin endometrium. Materials and Methods EnMSCs were treated with PRP in vitro, followed by measuring cell proliferation, migration, and adhesion by using CCK8, scratch, and adhesion test, respectively. Twenty patients undergoing in vitro fertilization (IVF) with refractory thin endometrium history were given PRP by infusion into the uterine cavity after the treatment of hormone replacement therapy (HRT). Results All components of PRP significantly stimulated the growth, migration, and adhesion of EnMSCs when compared with the negative control. Cell proliferation and migration were induced by PRP in a dose‐dependent manner with maximum proliferation at a 2% PRP dose. The clinical data showed that successful endometrial expansion and pregnancy were discovered in 12 patients after PRP infusion, and the pregnancy rate increased to 60%. Conclusion Intrauterine PRP infusion represents a new way for female patients with thin endometrium with poor response. This study lays the foundations for the potential treatment of thin endometrium with PRP in vivo.

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Section: Discussionmentioning

confidence: 99%

Investigation of platelet‐rich plasma in increasing proliferation and migration of endometrial mesenchymal stem cells and improving pregnancy outcome of patients with thin endometrium

Wang

Liu

Mou

et al. 2018

J of Cellular Biochemistry

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“…In addition to bone marrow, subacromial bursa has recently been proposed as an easily accessible alternative source for MSCs, highlighting its potential as a biologic augment in the setting of rotator cuff surgery (9)(10)(11). Despite these improvements in biologic therapies, extracellular matrix of specific scaffolds is still required to sufficiently deliver and retain applied MSCs to the targeted zone of repaired tissue (12). As artificial scaffolds require biocompatibility in order to ensure retaining the seeded MSCs and mimic natural properties, repair augmentation using demineralized bone matrix (DBM) scaffolds has recently emerged (12)(13)(14)(15)(16).…”

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confidence: 99%

“…Despite these improvements in biologic therapies, extracellular matrix of specific scaffolds is still required to sufficiently deliver and retain applied MSCs to the targeted zone of repaired tissue (12). As artificial scaffolds require biocompatibility in order to ensure retaining the seeded MSCs and mimic natural properties, repair augmentation using demineralized bone matrix (DBM) scaffolds has recently emerged (12)(13)(14)(15)(16). DBM scaffolds are a composite of collagen, several growth factors, along with calcium phosphate.…”

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confidence: 99%

“…DBM scaffolds are a composite of collagen, several growth factors, along with calcium phosphate. Its spongy structure allows for flexibility in the hydrated state, while maintaining an osteoinductive potential (12,13,16). In animal models, DBM has been shown to improve tendon-bone healing and to restore bone mineral density at the enthesis, highlighting its potential role as an augment in rotator cuff healing (14)(15)(16).…”

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confidence: 99%

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Concentrated Bone Marrow Aspirate and Subacromial Bursa-Derived Cells Demonstrate Similar Cellular Adhesion and Proliferation Potential on Demineralized Bone Matrix Scaffolds for Biologic Augmentation of Rotator Cuff Repair

Muench

Berthold

Kia

et al. 2020

Muscle Ligaments and Tendons J

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Background. Specific scaffolds are still required to sufficiently deliver and retain applied mesenchymal stem cells (MSCs) of various origins to the targeted zone of repaired tissue. The purpose of the study was to evaluate the demineralized bone matrix (DBM) as a scaffold for concentrated bone marrow aspirate (cBMA), subacromial bursa-derived cells (SBDCs), tenocytes, and osteoblasts. Methods. cBMA was harvested from the proximal humerus along with tenocytes and subacromial bursal tissue during arthroscopic shoulder surgery. Human osteoblasts were obtained from femoral condyles of patients undergoing total knee arthroplasty. Cells were seeded on a DBM scaffold. Cellular adhesion and proliferation assays were performed for each scaffold sample. Half of cBMA underwent arthroscopic simulation of being washed to mimic the intraoperative environment. Results. There was no significant difference in cellular adhesion potential on DBM scaffolds (p=0.168) when comparing SBDCs (mean±SD: 25,775±11,351), osteoblasts (mean±SD: 33,113±2,709), tenocytes (mean±SD: 25,000±3,864) and cBMA (mean±SD: 25,025±6,206). Osteoblasts (mean±SD: 0.279±0.009) showed a significantly higher proliferation potential (p=0.021, respectively) compared to SBDCs (mean±SD: 0.248±0.015), tenocytes (mean±SD: 0.239±0.011), and cBMA (mean±SD: 0.247±0.007). Histology sections and electron microscopy confirmed the presence of cells within the matrix of scaffolds. Conclusions. SBDCs and cBMA demonstrated high cellular adhesion and proliferation potential on DBM scaffolds.

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“…I believe that the model developed by Hoberman, Cirino, McCarthy, Cote, Pauzenberger, Beitzel, Mazzocca, and Dyrna in "Bone Marrow-Derived Mesenchymal Stromal Cells Enhanced by Platelet-Rich Plasma Maintain Adhesion to Scaffolds in Arthroscopic Simulation" 5 shows that permanence at the site of implantation is possible, and that not all scaffolds are the same. Fibrin scaffold and demineralized bone matrix are more effective than the normal human tendon, and that the addition of PRP enhanced cell adhesion, proliferation, and differentiation.…”

mentioning

confidence: 99%

Editorial Commentary: Paving a Road Requires a Well-Mixed Cement Stem Cells, Platelet-Rich Plasma, and Shoulder Rotator Cuff Healing

Maffulli

2018

Arthroscopy: The Journal of Arthroscopic & Related Surgery

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Bone Marrow-Derived Mesenchymal Stromal Cells Enhanced by Platelet-Rich Plasma Maintain Adhesion to Scaffolds in Arthroscopic Simulation

Cited by 23 publications

References 50 publications

Investigation of platelet‐rich plasma in increasing proliferation and migration of endometrial mesenchymal stem cells and improving pregnancy outcome of patients with thin endometrium

Investigation of platelet‐rich plasma in increasing proliferation and migration of endometrial mesenchymal stem cells and improving pregnancy outcome of patients with thin endometrium

Concentrated Bone Marrow Aspirate and Subacromial Bursa-Derived Cells Demonstrate Similar Cellular Adhesion and Proliferation Potential on Demineralized Bone Matrix Scaffolds for Biologic Augmentation of Rotator Cuff Repair

Editorial Commentary: Paving a Road Requires a Well-Mixed Cement Stem Cells, Platelet-Rich Plasma, and Shoulder Rotator Cuff Healing

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