Bone marrow-derived Schwann cells achieve fate commitment – a prerequisite for remyelination therapy

Shea, Graham Ka‐Hon; Tsui, Alex Y.P.; Chan, Ying-Shing; Shum, Dky

doi:10.1016/j.expneurol.2010.05.005

Cited by 47 publications

(81 citation statements)

References 43 publications

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“…[31][32][33] To study the possible synergistic or inhibitory effects of aSCs and BMSCs, both cell types were directly cocultured on chitosan films (Fig. 5).…”

Section: Discussionmentioning

confidence: 99%

In VitroEvaluation of Cell-Seeded Chitosan Films for Peripheral Nerve Tissue Engineering

Wróbel

Serra

Ribeiro-Samy

et al. 2014

Tissue Engineering Part A

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Natural biomaterials have attracted an increasing interest in the field of tissue-engineered nerve grafts, representing a possible alternative to autologous nerve transplantation. With the prospect of developing a novel entubulation strategy for transected nerves with cell-seeded chitosan films, we examined the biocompatibility of such films in vitro. Different types of rat Schwann cells (SCs)-immortalized, neonatal, and adult-as well as rat bone-marrow-derived mesenchymal stromal cells (BMSCs) were analyzed with regard to their cell metabolic activity, proliferation profiles, and cell morphology after different time points of mono-and cocultures on the chitosan films. Overall the results demonstrate a good cytocompatibility of the chitosan substrate. Both cell types were viable on the biomaterial and showed different metabolic activities and proliferation behavior, indicating cell-type-specific cell-biomaterial interaction. Moreover, the cell types also displayed their typical morphology. In cocultures adult SCs used the BMSCs as a feeder layer and no negative interactions between both cell types were detected. Further, the chitosan films allow neurite outgrowth from dissociated sensory neurons, which is additionally supported on film preseeded with SC-BMSC cocultures. The presented chitosan films therefore demonstrate high potential for their use in tissue-engineered nerve grafts.

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“…[31][32][33] To study the possible synergistic or inhibitory effects of aSCs and BMSCs, both cell types were directly cocultured on chitosan films (Fig. 5).…”

Section: Discussionmentioning

confidence: 99%

In VitroEvaluation of Cell-Seeded Chitosan Films for Peripheral Nerve Tissue Engineering

Wróbel

Serra

Ribeiro-Samy

et al. 2014

Tissue Engineering Part A

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“…2,9,10,17,21,26,42 Prior in vivo experiments from our group and others show some histological improvement after stem cell/Schwann cell therapies; however, the evidence for functional peripheral nerve recovery remains limited. 16,47,48 In the present study, we aimed to evaluate the potential of SCs derived from the isogenic nerve and skin precursors for improved sensory recovery in a standard sciatic nerve transection model.…”

mentioning

confidence: 88%

“…Co-cultures were maintained in SC medium containing 50 ng/ml NGF, and ascorbic acid (50 mg/ ml, Sigma) was added to induce myelination. 42 The coculture was maintained for 2 weeks, then fixed with 2% paraformaldehyde (PFA) in PBS, blocked/permeabilized with 2% BSA/ 0.3% Triton-X100 in PBS and stained for myelin basic protein (MBP) with secondary antibodies with an emission wavelength of 488 nm.…”

Section: In Vitro Myelinationmentioning

confidence: 99%

Sensory recovery after cell therapy in peripheral nerve repair: effects of naïve and skin precursor-derived Schwann cells

Shakhbazau

Mohanty²,

Kumar

et al. 2014

JNS

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Object Cell therapy is a promising candidate among biological or technological innovations sought to augment microsurgical techniques in peripheral nerve repair. This report describes long-term functional regenerative effects of cell therapy in the rat injury model with a focus on sensory recovery. Methods Schwann cells were derived from isogenic nerve or skin precursor cells and injected into the transected and immediately repaired sciatic nerve distal to the injury site. Sensory recovery was assessed at weeks 4, 7, and 10. Axonal regeneration was assessed at Week 11. Results By Week 10, thermal sensitivity in cell therapy groups returned to a level indistinguishable from the baseline (p > 0.05). Immunohistochemistry at 11 weeks after injury showed improved regeneration of NF+ and IB4+ axons. Conclusions: The results of this study show that cell therapy significantly improves thermal sensation and the number of regenerated sensory neurons at 11 weeks after injury. These findings contribute to the view of skin-derived stem cells as a reliable source of Schwann cells with therapeutic potential for functional recovery in damaged peripheral nerve.

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“…Previously, the fate of these cells seemed to be dependent on a permanent contact with their induction medium, as withdrawal of this medium resulted in dedifferentiation into myofibroblast phenotype (Zurita et al 2008). Recently, however, Shea et al (2010) were able to overcome this necessity by co-culturing the BMSC-derived SCs-like cells with dorsal root ganglion (DRG) neurons, achieving fate commitment in the rat bone marrow-derived SCs-like cells. The authors tested the hypothesis that the juxtacrine signaling provided from axons during SCs development would improve the maturation and fate commitment of bone marrow-derived SCs as well.…”

Section: Phenotype Stability Of Stem Cell-derived Cellsmentioning

confidence: 99%

“…The authors tested the hypothesis that the juxtacrine signaling provided from axons during SCs development would improve the maturation and fate commitment of bone marrow-derived SCs as well. Indeed, co-culture with embryonic rat DRG neurons was capable of producing SCs-like cells that maintained the fate commitment even after withdrawal of inducing factors (Shea et al 2010). While this may not be necessary for SCs-like cells derived using all protocols, it stresses the importance of confirming phenotypic stability of the derived cells.…”

Section: Phenotype Stability Of Stem Cell-derived Cellsmentioning

confidence: 99%

Stem Cell-Based Approaches to Improve Nerve Regeneration: Potential Implications for Reconstructive Transplantation?

Khalifian

Sarhane

Tammia

et al. 2014

Arch. Immunol. Ther. Exp.

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Reconstructive transplantation has become a viable option to restore form and function after devastating tissue loss. Functional recovery is a key determinant of overall success and critically depends on the quality and pace of nerve regeneration. Several molecular and cell-based therapies have been postulated and tested in pre-clinical animal models to enhance nerve regeneration. Schwann cells remain the mainstay of research focus providing neurotrophic support and signaling cues for regenerating axons. Alternative cell sources such as mesenchymal stem cells and adipose-derived stromal cells have also been tested in pre-clinical animal models and in clinical trials due to their relative ease of harvest, rapid expansion in vitro, minimal immunogenicity, and capacity to integrate and survive within host tissues, thereby overcoming many of the challenges faced by culturing of human Schwann cells and nerve allografting. Induced pluripotent stem cell-derived Schwann cells are of particular interest since they can provide abundant, patient-specific autologous Schwann cells. The majority of experimental evidence on cell-based therapies, however, has been generated using stem cell-seeded nerve guides that were developed to enhance nerve regeneration across "gaps" in neural repair. Although primary end-to-end repair is the preferred method of neurorrhaphy in reconstructive transplantation, mechanistic studies elucidating the principles of cell-based therapies from nerve guidance conduits will form the foundation of further research employing stem cells in end-to-end repair of donor and recipient nerves. This review presents key components of nerve regeneration in reconstructive transplantation and highlights the pre-clinical studies that utilize stem cells to enhance nerve regeneration.

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Bone marrow-derived Schwann cells achieve fate commitment – a prerequisite for remyelination therapy

Cited by 47 publications

References 43 publications

In VitroEvaluation of Cell-Seeded Chitosan Films for Peripheral Nerve Tissue Engineering

In VitroEvaluation of Cell-Seeded Chitosan Films for Peripheral Nerve Tissue Engineering

Sensory recovery after cell therapy in peripheral nerve repair: effects of naïve and skin precursor-derived Schwann cells

Stem Cell-Based Approaches to Improve Nerve Regeneration: Potential Implications for Reconstructive Transplantation?

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