Bone marrow-derived versus adipose-derived stem cells in wound healing: value and route of administration

Abstract

The stem cells with their distinct ability of self-renewal and differentiation are considered an innovation in wound healing. However, there is lack of studies comparing the differential effect of the type and administration route of stem cells in the wound healing context. Thus, the current study has been designed to elucidate the effect of two of the most important stem cell types-the bone marrow-derived and adipose-derived stem cells in full thickness wound healing-and to evaluate, in this optimized wound model, the effectiveness of intradermal versus intravenous routes using H&E, Masson's trichrome, and PKH26-stained sections. It also evaluated the immunohistochemical expression of the stem cell-related surface markers-Ki67, CD71, CD146, CD90, and CD163-and also assessed the level of TNFα and gene expression of NF-κB as two important inflammatory markers. The study revealed that the adipose stem cell groups have shown statistically significant improvement in inflammation, granulation tissue re-organization, and collagen deposition relative to their bone marrow-treated counterparts. The intradermally treated adipose stem cell group, in particular, has demonstrated the most supreme features regarding the expression of the proliferation-related surface markers Ki67 and CD71 as well as in the expression of CD90 in keratinocytes and hair follicle dermal sheaths. The same group has shown the lowest level of TNFα and the best outcome in the parameters of neo-epidermal thickness, granulation tissue re-organization, and pattern of collagen deposition. The systemically treated wounds have displayed superior expression of CD146-positive endothelial cells and dermal fibroblasts as well as better expression of CD163+ macrophages.

Human Adipose Mesenchymal Stem Cells Show More Efficient Angiogenesis Promotion on Endothelial Colony-Forming Cells than Umbilical Cord and Endometrium

“…It is indicated that MSCs from placental amnion, chorion, and umbilical cord exert similar beneficial effects on wound closure and neovascularization [28], and bone marrow, adipose tissue, and dental pulp are also proved to serve as a universal MSC source for burn wound healing [29]. Conversely, Aboulhoda and Abd El Fattah demonstrated that ADSCs show statistically significant improvement in wound healing than BMSCs [30]. And adipose-derived MSCs are also proved to exhibit greater angiogenic potential in comparison with BMSCs [31] and UCMSCs [22].…”

Nebivolol protects against cyclophosphamide‐induced nephrotoxicity through modulation of oxidative stress, inflammation, and apoptosis

“…Digital images were uniformly acquired and snapped at x400 magnification by Olympus IX51 equipped with a DP72 device camera. The obtained data were calculated in six non‐overlapping fields per slide in each group 44 …”

Protective effect of tolvaptan against cyclophosphamide‐induced nephrotoxicity in rat models

“…All antibodies were purchased from Abcam ® (Cambridge). The color reaction was then made with HRP‐linked polymer detection system and counterstained with hematoxylin as previously described 29 . Paraffin‐embedded mouse spleen tissue was used as a positive control.…”