2000
DOI: 10.1016/s0272-2712(18)30083-0
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Bone Marrow Engraftment Analysis After Allogeneic Bone Marrow Transplantation

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Cited by 61 publications
(30 citation statements)
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“…Pre-transplant recipient (and donor) DNAs were submitted to the Molecular Genetic Pathology Laboratory at the Cleveland Clinic Foundation for STR analysis using the PowerPlex 16 System to identify informative loci that could be used to monitor engraftment in post-transplant specimens. 6 Using the PowerPlex 16 System, the amelogenin primers generate an X-specific product (ϳ104 bp) in the normal male (Figure 2A) and normal female ( Figure 2B) but not in the case study male ( Figure 2C). A Y-specific product (ϳ110 bp) is present in the normal male ( Figure 2A) and case study male ( Figure 2C) but not in the female ( Figure 2B).…”
Section: Allele-specific Pcrmentioning
confidence: 99%
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“…Pre-transplant recipient (and donor) DNAs were submitted to the Molecular Genetic Pathology Laboratory at the Cleveland Clinic Foundation for STR analysis using the PowerPlex 16 System to identify informative loci that could be used to monitor engraftment in post-transplant specimens. 6 Using the PowerPlex 16 System, the amelogenin primers generate an X-specific product (ϳ104 bp) in the normal male (Figure 2A) and normal female ( Figure 2B) but not in the case study male ( Figure 2C). A Y-specific product (ϳ110 bp) is present in the normal male ( Figure 2A) and case study male ( Figure 2C) but not in the female ( Figure 2B).…”
Section: Allele-specific Pcrmentioning
confidence: 99%
“…However, DNA typing applications in pathology are gaining in popularity and include testing to monitor for bone marrow engraftment in patients undergoing allogeneic bone marrow transplants, evaluating the origin of tumors inadvertently transmitted by solid organ transplantation, and resolving sample mix-ups. 5,6 Several studies have reported mutations in the Y homologue of the amelogenin gene which can cause typing of males as females. [7][8][9][10] Failure to amplify the Y homologue could result in dire consequences if used in cases of criminal investigation or for gender identification in the setting of prenatal diagnosis.…”
mentioning
confidence: 99%
“…10 Briefly, DNA was extracted from bone marrow samples, from FACS-separated mononuclear cells as well as from CD3 + and CD3 − lymphocytes. Furthermore, buccal swabs were collected from the donors and recipients to establish their genotypes prior to transplantation.…”
Section: Chimerism Assaymentioning
confidence: 99%
“…Bone marrow engraftment monitoring using polymerase chain reaction (PCR) amplification targeting various polymorphic loci followed by capillary electrophoresis of fluorescently labeled PCR products has become a standard laboratory procedure used in the management of patients receiving allogeneic bone marrow transplants. 1,2 However, the choice of loci to be examined largely remains laboratory specific. Several commercially available kits, targeting either multiple short tandem repeats (STRs) or variable number of tandem repeats (VNTRs), have been used extensively for human identification purposes in the forensic community.…”
mentioning
confidence: 99%