Bone marrow macrophages induced to antigen presentation by lymphokines interact selectively with distinct T cells

Fischer, Hans; Reske‐Kunz, Angelika B.; Spaeth, E.; Rüde, Erwin

doi:10.1002/eji.1830150917

Cited by 4 publications

(1 citation statement)

References 16 publications

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“…injection of concanavalin A [6]. IFN-y-activated BMMQ, were not capable of inducing antigen-specific proliferation of every T cell clone tested, suggesting that distinct activation requirements exist for different T cells [7].…”

Section: Introductionmentioning

confidence: 99%

Granulocyte‐macrophage colony‐stimulating factor‐cultured bone marrow‐derived macrophages reveal accessory cell function and synthesis of MHC class II determinants in the absence of external stimuli

et al. 1988

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The antigen-mediated activation of a number of T cell clones by bone marrow (BM) cells cultivated in the presence of various colony-stimulating factor (CSF) preparations was investigated. BM macrophages (BMM phi) grown in L929 cell supernatant as a crude source of macrophage colony-stimulating factor (M-CSF) as well as BM cells propagated in the presence of recombinant M-CSF exhibited transient antigen presentation potential to some T cell clones, being maximal on day 7 and having declined to a low level by day 19 of in vitro culture. Treatment of these long-term-cultivated BMM phi populations with recombinant interferon-gamma (IFN-gamma) resulted in predominant antigen presentation capacity. In contrast, BM cells differentiated in the presence of recombinant granulocyte (G)M-CSF developed highly efficient accessory cell function to all T cell clones examined. This function became apparent earlier, was retained during the time period tested (up to day 19 of continuous culture) and did not require prior stimulation by IFN-gamma. The functionally competent cells were shown to belong to the monocyte/macrophage lineage. These findings are consistent with the demonstration of substantial levels of major histocompatibility complex (MHC) class II molecules synthesized by GM-CSF-cultured BM cells in the absence of exogenous IFN-gamma. In contrast, M-CSF grown BM cells synthesized only minute amounts of Ia antigens unless they were stimulated by IFN-gamma. Because GM-CSF-cultivated BM cells proved clearly superior to M-CSF-grown and IFN-gamma-activated BM cells with respect to antigen-presenting capacity but exhibited lower levels of MHC class II molecules, other properties acting in addition to surface Ia antigens might be responsible for their pronounced T cell accessory function.

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Section: Introductionmentioning

confidence: 99%

Granulocyte‐macrophage colony‐stimulating factor‐cultured bone marrow‐derived macrophages reveal accessory cell function and synthesis of MHC class II determinants in the absence of external stimuli

et al. 1988

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Induction of interferon‐γ production and Ia expression by interleukin 1 in bone marrow culture cells

1987

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Colony-stimulating factor-induced bone marrow (BM) cultures are a good source of antigen-presenting macrophages. However, they failed to present antigen to T cell clones when antigen was introduced as a short pulse only. Adding interleukin 1 (IL1) to BM culture cells before antigen pulse restored their antigen-presenting activity concomitant to a 2-10-fold increase in Ia antigenicity. We performed a series of experiments to test the mechanism of this IL1-induced activation. Our findings suggest that IL1 influences Ia expression and antigen-specific T cell proliferation by inducing interferon-gamma (IFN-gamma) production. IFN-gamma is produced in this system probably by residual Thy-1-positive cells in the BM cell culture.

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Cloned T helper cells reverting to a resting state develop increasing sensitivity in their antigen‐mediated interaction with accessory cells

1988

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A cloned murine T cell line, KIII5, specific for the polypeptide poly-L(Tyr,Glu)-poly-D,L-Ala--poly-L-Lys [(T,G)-A--L] was compared at different stages after antigenic stimulation with respect to the conditions required for the reinduction of growth by varying concentrations of antigen presented on different types of accessory cells (AC). We show that the dose of antigen necessary for inducing half maximal proliferation in the presence of splenic AC shifts to considerably lower concentrations when the T cell blasts revert to a resting state (100 micrograms/ml on day 7 to 10 micrograms/ml on day 21-35). During the same time period the expression of interleukin 2 (IL2) receptor and the reactivity to IL2 decline. However, no direct correlation between the increasing sensitivity to antigen and the decreasing reactivity to IL2 appears to exist. With peritoneal AC "early" T cells (day 7) did not respond to (T,G)-A--L at all, but in the course of "aging" responsiveness increased and finally reached the same level as in the presence of splenic AC, although at a higher antigen dose (100 micrograms/ml on day 35-45). Furthermore, the antigen-induced proliferation of "aging" T cells became more resistant to inhibition both by anti-L3T4 and anti-T cell receptor antibodies. Two alternative interpretations of these data are possible: antigen-activated T cells, while gradually reverting to a resting state, interact more avidly with antigen-presenting cells or the triggering threshold of the T cells is decreasing.

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Bone marrow macrophages induced to antigen presentation by lymphokines interact selectively with distinct T cells

Cited by 4 publications

References 16 publications

Granulocyte‐macrophage colony‐stimulating factor‐cultured bone marrow‐derived macrophages reveal accessory cell function and synthesis of MHC class II determinants in the absence of external stimuli

Granulocyte‐macrophage colony‐stimulating factor‐cultured bone marrow‐derived macrophages reveal accessory cell function and synthesis of MHC class II determinants in the absence of external stimuli

Induction of interferon‐γ production and Ia expression by interleukin 1 in bone marrow culture cells

Cloned T helper cells reverting to a resting state develop increasing sensitivity in their antigen‐mediated interaction with accessory cells

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