Bone marrow stem cells‐derived microvesicles protect against renal injury in the mouse remnant kidney model

Abstract

This study showed MV protects against renal injury induced by 5/6 Nx, which could mimic the role of MSC in kidney repair. The research showed a newly potential therapeutic approach to kidney diseases.

Therapeutic effects of mesenchymal stem cell-derived microvesicles on pulmonary arterial hypertension in rats

“…Isolation, culture, and characterization of MSCs MSCs were isolated from the bone marrow of rats as we previously described [7] . Briefly, after the rat was sacrificed, the femurs and tibias were removed and cleaned.…”

“…Flow cytometry analysis was performed to identify cultured cells [7] . In brief, cells were detached using trypsin-EDTA, centrifuged and incubated with PE-conjugated antibodies in a final volume of 100 mL for 30 min at 4 ºC in the dark.…”

“…Collection and characterization of MSC-MVs MSC-MVs were obtained from MSC conditioned culture medium according to a previously described protocol with slight modifications [7] . Briefly, MSCs were cultured in serumfree α-MEM for 24 h. Then, the cell culture medium was collected, centrifuged at 2000×g for 20 min to remove cells and debris.…”

“…The therapeutic effects of MVs have been actively investigated in animal models of various diseases. For example, MSC-MV transplantation could protect kidneys from ischemia/reperfusion-induced injury and chronic renal damage by stimulating proliferation and inhibiting the apoptosis of tubular epithelial cells [7][8][9] . Intravenous administration of MSC-MVs has been shown to improve neurogenesis and angiogenesis in an ischemic stroke model [10] .…”

Posttransplantation Bone Marrow Assessment by Quantifying Hematopoietic Cell–Derived mRNAs in Plasma Exosomes/Microvesicles

“…However, the BM cells in these reports were stromal cells (12 ), stem cells (13 ), dendritic cells (14 ), and mesenchymal stem cells (15 ), not HPC. DEFA3 is a gene of microbicidal and cytotoxic peptides found in the granules of neutrophils and precursor myeloid cells.…”