Bone Marrow Transplantation in the Rat

In this study we examined the effects of acute graft-vs-host disease (aGVHD) on the Brown Norway (BN) rat liver. When clinical signs of the disease appeared, rats were inoculated with fluorescent latex beads and 30 min later nonparenchymal cells were isolated from the liver. The cells were then analyzed via flow cytometry, histochemistry, and electron microscopy. Flow cytometry demonstrated that 58% of the cells from the 80 ml/min elutriation fraction (normally rich in Kupffer cells) of the non-GVHD liver had high fluorescence intensity compared to 8% in rats with aGVHD. Determination of the cellular composition of the various fractions with electron microscopy confirmed flow cytometry observations in that only 9% of the 80 ml/min elutriation fraction of GVHD livers had peroxidase-positive rough ER and the morphological appearance of macrophages as compared to 60% in the non-GVHD liver. The low percentage of fluorescent-positive Kupffer cells in the 80 ml/min elutriation fraction of the GVHD liver is attributed to a massive lymphocytic invasion of the liver and not necessarily to a defect in the mononuclear phagocyte system.

show abstract

Effects of Acute Graft-vs-Host Disease on the Liver of the Brown Norway Rat

Hightower

Earnest

Martens

et al. 1987

Journal of Leukocyte Biology

View full text Add to dashboard Cite

show abstract

Feline bone marrow transplantation: its use in FIV-infected cats

Yamamoto¹,

Pu²,

Arai³

et al. 1998

Veterinary Immunology and Immunopathology

View full text Add to dashboard Cite

Bone marrow transplantation in the rat

Renkonen

Häyry

1989

APMIS

View full text Add to dashboard Cite

We have isolated inflammatory leukocytes from various lymphoid and parenchymal organs after total body irradiation and bone marrow transplantatation from either an allogeneic or syngeneic strain and tested their ability to perform lytic functions in vitro. No direct lytic activity (i.e. cytotoxic T lymphocytes, CTL) to relevant strain‐derived target cells in the lymphoid or parenchymal target organs was seen preceding or during acute graft‐versus‐host disease (aGVHD). Instead, the leukocytes of the spleen and blood and the inflammatory cells of liver and lungs were efficient effector cells against recipient‐derived target cells in the presence of relevant antibody (antiboy dependent cellular cytotoxicity, ADCC). The NK activity against YAC‐1 (natural killer, NK) target cells was first high in the spleen, but when the aGHVD appeared in the allograft marrow recipients the NK activity decreased in the spleen with a concomitant increase in the liver, but not in the other parenchymal target organs. At the same time no NK activity was seen in the syngeneic marrow graft recipients' parenchymal organs. These observations suggest functional differences in the structure of inflammation in the different target organs of aGVHD.

show abstract

scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.

Contact Info

customersupport@researchsolutions.com

10624 S. Eastern Ave., Ste. A-614

Henderson, NV 89052, USA

This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.

Blog Terms and Conditions API Terms Privacy Policy Contact Cookie Preferences Do Not Sell or Share My Personal Information

Made with 💙 for researchers

Part of the Research Solutions Family.

Bone Marrow Transplantation in the Rat

Cited by 5 publications

References 18 publications

Effects of Acute Graft-vs-Host Disease on the Liver of the Brown Norway Rat

Effects of Acute Graft-vs-Host Disease on the Liver of the Brown Norway Rat

Feline bone marrow transplantation: its use in FIV-infected cats

Bone marrow transplantation in the rat

Contact Info

Product

Resources

About