Bone Matrix in the Solid State: Geometric Influence on Differentiation of Fibroblasts

Rudresha, A H

doi:10.1016/b978-0-12-005215-8.50007-3

Cited by 68 publications

(42 citation statements)

References 35 publications

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“…In the mice, the new osteoids on the surface of DDM/rhBMP-2 implants at 2 weeks showed cartilaginous bone formation with several chondrocytes and new vascular invasion into the collagenolytic resorbed space that is consistent with the previous studies of Reddi and Murata (Fig. 3) 15,31,46,47) .…”

Section: Discussionsupporting

confidence: 79%

Demineralized Dentin Matrix as a Carrier of Recombinant Human Bone Morphogenetic Proteins: <i>in Vivo</i> Study

Kim

Jun

et al. 2018

J. Hard Tissue Biology.

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This study aimed to evaluate the effi cacy of rabbit demineralized dentin matrix (DDM) as a recombinant human bone morphogenetic protein-2 (rhBMP-2) carrier using the subcutaneous tissues of mice and rabbit calvarial critical-sized defects. DDM of rabbit, combined with rhBMP-2 (DDM/rhBMP-2) was transplanted into the subcutaneous tissues of 6 mice and 6 rabbit calvarial critical-sized defects (DDM = 0.03 g, control; DDM/rhBMP-2 = 0.03 g of DDM, 0.2 mg/ml, 5.0 μg of rhBMP-2, experimental). Both DDM and DDM/rhBMP-2 was transplanted into the left and right subcutaneous tissues of mice symmetrically. For rabbits, 4 round critical-sized defects (8 mm diameter) were formed on the exposed skull. DDM was transplanted into the 2 defects on the left sides (n = 12) and DDM/rhBMP-2 into the right sides (n = 12). Two animals among 6 mice and 6 rabbits were sacrifi ced respectively at the 1, 2, and 4 experimental weeks for the histological and histomorphometrical evaluations with hematoxylin and eosin staining. Tissues from rabbits were imaged via micro-computed tomography (μCT). DDM/rhBMP-2 in mice induced new bone formation at 2 weeks and maturation with bone marrow at 4 weeks. DDM/rhBMP-2 in rabbit calvarium induced new bone formation remarkably at 4 weeks 21.77-47.99% compared to the DDM. These observations suggest that DDM could be considered a potential carrier of rhBMP-2. The rhBMP-2 loaded on DDM enhanced bone formation.

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Section: Discussionsupporting

confidence: 79%

Demineralized Dentin Matrix as a Carrier of Recombinant Human Bone Morphogenetic Proteins: <i>in Vivo</i> Study

Kim

Jun

et al. 2018

J. Hard Tissue Biology.

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“…An important function for an osteogenic delivery system is the initiation of optimal osteoinductivity with relatively low doses of recombinant BMPs. Additional considerations of importance are the surface characteristics and geometry of the substratum, which were found to be of critical importance in the induction of bone by BMPs (Reddi, 1974;Sampath and Reddi, 1984;Ripamonti et al, 1992b; Eeden and Ripamonti, 1994).…”

mentioning

confidence: 99%

Tissue Engineering, Morphogenesis, and Regeneration of the Periodontal Tissues By Bone Morphogenetic Proteins

Ripamonti

Reddi

1997

Critical Reviews in Oral Biology & Medicine

164

129

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Tissue engineering is the emerging field of science developing techniques for fabrication of new tissues for replacement based on principles of cell and developmental biology and biomaterials. Morphogenesis is the cascade of pattern formation and the attainment of form of the various organs and the organism as a whole. The periodontium consists of the periodontal ligament, cementum, and alveolar bone. Bone has considerable potential for regeneration and therefore is a prototypic model for tissue engineering. The three main ingredients for tissue engineering are regulatory signals, responding stem cells, and extracellular matrix. Recent advances in molecular biology of the bone morphogenetic proteins (BMPs) have set the stage for tissue engineering of bone and related tissues, including the periodontium. Bone-derived BMPs, with a collagenous matrix as carrier, induced cementum and alveolar bone regeneration in surgically created furcation defects in the primate. It is noteworthy that there was morphogenesis of periodontal ligament and a faithful insertion of Sharpey's fibers into cementum. In the same furcation model, recombinant human osteogenic protein-l (rhOP-l, also known as BMP-7), in conjunction with the collagenous carrier, induced extensive cementogenesis with insertion of Sharpey's fibers into the newly formed cementum. The observation that BMPs induce cementogenesis and periodontal ligament formation indicates that these proteins may have multiple functions in vivo not limited to cartilage and bone induction. The rapid advances in the molecular biology of BMPs and their receptors bode well for novel strategies to engineer the regeneration of the periodontal tissues.

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“…The decalcified dentin (DDM) was known to be more active bone-inducing matrix than the calcified dentin (Yeoman & Urist, 1967), and roll type of decalcified dentin membrane revealed better activity of bone induction (Inoue et al, 1986). Very interestingly, the demineralized treatment for bone and dentin increased their osteoinductivity and decreased their antigenesity (Reddi, 1974). These facts are scientifically very important for the processing procedures of hard tissue-derived graft materials (Kim et al, 2010;Murata et al, 2010a).…”

Section: Wwwintechopencommentioning

confidence: 94%

Human Dentin as Novel Biomaterial for Bone Regeneration

Murata¹,

Akazawa²,

Mitsugi³

et al. 2011

Biomaterials - Physics and Chemistry

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Bone Matrix in the Solid State: Geometric Influence on Differentiation of Fibroblasts

Cited by 68 publications

References 35 publications

Demineralized Dentin Matrix as a Carrier of Recombinant Human Bone Morphogenetic Proteins: <i>in Vivo</i> Study

Demineralized Dentin Matrix as a Carrier of Recombinant Human Bone Morphogenetic Proteins: <i>in Vivo</i> Study

Tissue Engineering, Morphogenesis, and Regeneration of the Periodontal Tissues By Bone Morphogenetic Proteins

Human Dentin as Novel Biomaterial for Bone Regeneration

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