Bone mesenchymal stem cell functions on the hierarchical micro/nanotopographies of the Ti-6Al-7Nb alloy

Ren, Nan; Li, Yongfeng; Shen, Shuning; Niu, Qiang; Zhao, Yimin; Kong, Liang

doi:10.1016/j.bjoms.2014.08.022

Cited by 11 publications

(10 citation statements)

References 24 publications

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“…Some studies have documented the effects of micro/nano-topographies on osteogenesis in vitro. [16][17][18] The micro and nanoscale surfaces matched the natural structure of bone, which consisted of hierarchical structures such as nano-structures (including collagen, non-collagenous organic proteins, and mineral crystals) and micro-structures (including haversian systems, osteons, and single trabeculae). Thirdly, micro/nano hierarchical surfaces have great potential for improving spreading and osteogenic differentiation of BMSC sheets.…”

Section: Discussionmentioning

confidence: 96%

“…In addition, previous studies conrmed that micro and nano structures on Ti substrates could enhance the biological responses of BMSCs. [16][17][18][19] It was reported that specic micro and nano structures with 80 nm grains signicantly enhanced osteogenic differentiation of BMSCs compared with 20 nm and 40 nm grains. 20 Nevertheless, to the best of our knowledge, no research has been conducted to investigate the effects of TiO 2 nanoparticle coated titanium implants on osseointegrating functionality of cell sheets in vitro and in vivo.…”

Section: Introductionmentioning

confidence: 99%

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Improved osseointegrating functionality of cell sheets on anatase TiO₂ nanoparticle surfaces

Wang

Jiang

et al. 2017

RSC Adv.

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Section: Discussionmentioning

confidence: 96%

Section: Introductionmentioning

confidence: 99%

Improved osseointegrating functionality of cell sheets on anatase TiO₂ nanoparticle surfaces

Wang

Jiang

et al. 2017

RSC Adv.

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“…Recently, titanium and a variety of titanium alloys, including Ti-6Al-4V, Ti-6Al-4V-ELI (extra low interstitial), Ti-6Al-7Nb, and Ti-13Nb-13Zr, have received significant attention due to their exceptional material properties. In particular, they demonstrate high corrosion resistance, high biocompatibility, low stiffness, and low density [2,[35][36][37].…”

Section: Introductionmentioning

confidence: 99%

A Novel Methodology for Economical Scale-Up of TiO₂ Nanotubes Fabricated on Ti and Ti Alloys

Khaw

Curioni

Skeldon

et al. 2019

Journal of Nanotechnology

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The prospective use of nanotechnology for medical devices is increasing. While the impact of material surface nanopatterning on the biological response is convincing, creating a large surface area with such nanotechnology remains an unmet challenge. In this paper, we describe, for the first time, a reproducible scale-up manufacturing technique for creating controlled nanotubes on the surfaces of Ti and Ti alloys. We describe an average of approximately 7.5-fold increase in cost and time efficiency with regards to the generation of 20, 50, and 100 nm diameter nanotubes using an anodisation technique. These novel materials have great potential in the medical field through their influence on cellular activity, in particular, protein absorption, focal adhesion, and osteoinduction. In this paper, we provide a step-by-step guide to optimise an anodisation system, starting with design rationale, proof of concept, device upscaling, consistency, and reproducibility check, followed by cost and efficiency analysis. We show that the optimised device can produce a high number of anodised specimens with customisable specimen shape at reduced cost and time, without compromising the repeatability and consistency. The device can fabricate highly uniform and vertically oriented TiO2 nanotube layer with desired pore diameters.

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Section: Discussionmentioning

confidence: 91%

“…It is evident that mesenchymal stromal cells adhere to titanium surfaces and the development of cell protrusions on these surfaces was verified by scanning electron microscopy in several in vitro studies. [30][31][32][33][34] Differences in roughness of titanium surfaces seem to have a considerable effect on differentiation progresses of attached hMSC. Our results suggest that native polished Ti6Al4V-alloy surfaces at an average roughness of 1.4 mm induce osteogenic differentiation and proliferation greater than sandblasted at 5 mm.…”

Section: Discussionmentioning

confidence: 99%

Osteogenic differentiation and proliferation of bone marrow‐derived mesenchymal stromal cells on PDLLA + BMP‐2‐coated titanium alloy surfaces

Haversath

Hülsen

Böge

et al. 2015

J Biomedical Materials Res

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RhBMP-2 is clinically applied to enhance bone healing and used in combination with titanium fixation implants. The purpose of this in vitro study was to compare the osteogenic differentiation and proliferation of hMSC on native polished versus sandblasted titanium surfaces (TS) and to test their behavior on pure poly-D,L-lactide (PDLLA) coated as well as PDLLA + rhBMP-2 coated TS. Furthermore, the release kinetics of PDLLA + rhBMP-2-coated TS was investigated. Human bone marrow cells were obtained from three different donors (A: male, 16 yrs; B: male, 27 yrs, C: male, 49 yrs) followed by density gradient centrifugation and flow cytometry with defined antigens. The cells were seeded on native polished and sandblasted TS, PDLLA-coated TS and PDLLA + rhBMP-2-coated TS. Osteogenic differentiation (ALP specific activity via ALP and BCA assay) and proliferation (LDH cytotoxicity assay) was examined on day 7 and 14 and release kinetics of rhBMP-2 was investigated on day 3, 7, 10, and 14. We found significant higher ALP specific activity and LDH activity on native polished compared to native sandblasted surfaces. PDLLA led to decreased ALP specific and LDH activity on both surface finishes. Additional rhBMP-2 slightly diminished this effect. RhBMP-2-release from coated TS decreased nearly exponentially with highest concentrations at the beginning of the cultivation period. The results of this in vitro study suggest that native TS stimulate hMSC significantly stronger toward osteogenic differentiation and proliferation than rhBMP-2 + PDLLA-layered TS in the first 14 days of cultivation. The PDLLA-layer seems to inhibit local hMSC differentiation and proliferation.

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Bone mesenchymal stem cell functions on the hierarchical micro/nanotopographies of the Ti-6Al-7Nb alloy

Cited by 11 publications

References 24 publications

Improved osseointegrating functionality of cell sheets on anatase TiO₂ nanoparticle surfaces

Improved osseointegrating functionality of cell sheets on anatase TiO₂ nanoparticle surfaces

A Novel Methodology for Economical Scale-Up of TiO₂ Nanotubes Fabricated on Ti and Ti Alloys

Osteogenic differentiation and proliferation of bone marrow‐derived mesenchymal stromal cells on PDLLA + BMP‐2‐coated titanium alloy surfaces

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Bone mesenchymal stem cell functions on the hierarchical micro/nanotopographies of the Ti-6Al-7Nb alloy

Cited by 11 publications

References 24 publications

Improved osseointegrating functionality of cell sheets on anatase TiO2 nanoparticle surfaces

Improved osseointegrating functionality of cell sheets on anatase TiO2 nanoparticle surfaces

A Novel Methodology for Economical Scale-Up of TiO2 Nanotubes Fabricated on Ti and Ti Alloys

Osteogenic differentiation and proliferation of bone marrow‐derived mesenchymal stromal cells on PDLLA + BMP‐2‐coated titanium alloy surfaces

Contact Info

Product

Resources

About

Improved osseointegrating functionality of cell sheets on anatase TiO₂ nanoparticle surfaces

Improved osseointegrating functionality of cell sheets on anatase TiO₂ nanoparticle surfaces

A Novel Methodology for Economical Scale-Up of TiO₂ Nanotubes Fabricated on Ti and Ti Alloys