Bone Morphogenetic Protein-15 in the Zebrafish Ovary: Complementary Deoxyribonucleic Acid Cloning, Genomic Organization, Tissue Distribution, and Role in Oocyte Maturation

Clelland, Eric; Kohli, Gurneet; Campbell, R. Keith; Sharma, Shweta; Shimasaki, Shunichi; Peng, Chun

doi:10.1210/en.2005-1017

Cited by 89 publications

(68 citation statements)

References 51 publications

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“…In fact, previous studies on zebrafish have described how treatment with recombinant human TGFB1 inhibits oocyte maturation reducing Lh activity by decreasing lhcgr and cbr1l mRNA levels, and have shown decreased basal and hCG-induced paqr8 mRNA levels in zebrafish follicles (Kohli et al 2005). Similar results have also been obtained with recombinant human BMP15, which also reduced hCG-induced oocyte maturation (Clelland et al 2006). Moreover, the knockdown of bmp15 in oocytes, by antisense oligonucleotides, increased paqr8 expression (Tan et al 2009), and the inhibition of bmp15 expression or function in class IIIa follicles has been found to induce their sensitivity to MIH (Clelland et al 2006(Clelland et al , 2007.…”

Section: Discussionsupporting

confidence: 77%

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Increase of fecundity by probiotic administration in zebrafish (Danio rerio)

Gioacchini

Maradonna²,

Lombardo³

et al. 2010

Reproduction

108

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It is well known established that reproduction is sensitive to the state of energy reserves, and that there is a balance between energy homeostasis and fertility. In this view, this study examined the effects of the probiotic Lactobacillus rhamnosus, as a feed additive, on zebrafish (Danio rerio) fecundity. Ten days of probiotic treatment modulate the gene expression of neuropeptide hormones and metabolic signals, such as kiss1, kiss2 and leptin both at the CNS level and at the peripheral level. The increase in fecundity brought about by the probiotic was demonstrated by the higher number of ovulated eggs in vivo and by the higher germinal vesicle breakdown rate obtained with the in vitro maturation assay. The increase in oocyte maturation was associated with increased transcription of genes coding for signals which induce the maturation phase, such as lhcgr, cbr1l, and paqr8 genes, concomitant with a decreased transcription of genes coding for local factors which prevent oocyte maturation, such as tgfb1, gdf9, and bmp15. In conclusion, all these findings highlight the action of L. rhamnosus both on the endocrine system and at the local level by inducing oocyte maturation. The significance of the results herein obtained underlined the importance of diet in the reproductive process, supporting the hypothesis that feed additives can improve fecundity. Considering that the zebrafish has been clearly established as a vertebrate model for biomedical research, these results support the potentiality of feed additives such as probiotics, frequently used in the human diet, as a new technology to improve reproduction in all vertebrates, including humans.

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Section: Discussionsupporting

confidence: 77%

“…In vitro maturation assays were conducted as described previously (Clelland et al 2006). The ovaries were teased into separate follicles using transfer pipettes (Samco Scientific Corp., San Fernando, CA, USA) without trypsinization.…”

Section: Follicles In Vitro Maturation Assaymentioning

confidence: 99%

Increase of fecundity by probiotic administration in zebrafish (Danio rerio)

Gioacchini

Maradonna²,

Lombardo³

et al. 2010

Reproduction

108

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show abstract

“…These data are not in agreement with some studies that report an involvement of BMP-15 in oocyte maturation and cumulus expansion in mice (Yoshino et al 2006) and pigs (Li et al 2008). However, recent studies in zebrafish have demonstrated that BMP-15 exerts a physiological role to inhibit oocyte maturation (Clelland et al 2006(Clelland et al , 2007Peng et al 2009). In this species, BMP-15 has shown the ability to prevent premature oocyte maturation, which has been considered important for the maintenance of oocyte quality and subsequent ovulation and fertilization (Peng et al 2009).…”

Section: Discussioncontrasting

confidence: 76%

BMPRIB and BMPRII mRNA expression levels in goat ovarian follicles and the in vitro effects of BMP-15 on preantral follicle development

et al. 2012

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This study evaluated the levels of bone morphogenetic protein receptors BMPRIB and BMPRII mRNA in goat follicles and the effects of bone morphogenetic protein-15 (BMP-15) on the in vitro development of cultured preantral follicles. Real-time polymerase chain reaction (PCR) was used to analyze the levels of BMPRIB and BMPRII mRNA in caprine preantral follicles and in small and large antral follicles. Preantral follicles (≥150 μm) were also isolated from goat ovaries and cultured for 18 days in α-MEM(+) supplemented with or without BMP-15 (10, 50, or 100 ng/ml). At the end of culture, some follicles were fixed for ultrastructural evaluation. Real-time PCR showed a reduction in BMPRII mRNA levels from the primary to secondary follicles. Higher levels of BMPRIB mRNA were observed in granulosa/theca cells from large antral follicles compared with small antral follicles. Moreover, BMPRII mRNA was expressed to a greater extent in cumulus-oocyte complexes from large antral follicles than in their respective granulosa/theca cells. In culture, 50 ng/ml BMP-15 positively influenced antral cavity formation and follicle growth after 18 days and also maintained follicular integrity. Thus, BMPRIB and BMPRII mRNAs are present in all follicular categories. BMP-15 (50 ng/ml) stimulates growth, antrum formation and the ultrastructural integrity of isolated caprine preantral follicles after 18 days of culture.

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“…BMP15 is produced by oocytes and plays important roles within the ovary, such as promoting early folliculogenesis and enhancing cumulus cell expansion (Peng et al, 2009). Previous reports have revealed that BMP15 prevented precocious oocyte maturation in zebrafish to maintain oocyte quality (Clelland et al, 2006;Peng et al, 2009). In addition, c-kit (also known as CD117) is a member of the tyrosine kinase and the receptor of the SCF.…”

Section: Discussionmentioning

confidence: 99%

BMP15 Gene Is Activated During Human Amniotic Fluid Stem Cell Differentiation into Oocyte-Like Cells

Cheng

Chen

et al. 2012

DNA and Cell Biology

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The generation of oocyte-like cells (OLCs) from stem cell differentiation in vitro provides an optimal approach for studying the mechanism of oocyte development and maturation. The aim of this study was to investigate the activation of bone morphogenetic protein 15 gene (BMP15) during the differentiation of human amniotic fluid stem cells (hAFSCs) into OLCs. After 15 days of differentiation, OLCs with a diameter of 50-60 μm and zona pellucida (ZP)-like morphology were observed. Reverse transcription-polymerase chain reaction (RT-PCR) analysis showed the BMP15 was activated from approximately day 10 of differentiating hAFSCs and thereafter. The reporter construct pBMP15-enhanced green fluorescent protein (EGFP) was transiently transfected into the differentiated hAFSCs and the EGFP expression driven by the BMP15 promoter was positive in the OLCs. Moreover, RT-PCR analysis showed that the oocyte-specific markers including ZP1, ZP2, ZP3, and c-kit were expressed in the differentiated hAFSCs, and the immunofluorescence assay confirmed that the ZP2 was detected in the OLCs. Quantitative RT-PCR revealed that ZP2 and ZP3 were significantly elevated in the differentiated hAFSCs. Further, in the OLCs derived from hAFSCs, the BMP15 promoter directing the EGFP reporter was colocalized with ZP2. Together, these results illustrated that the BMP15 could be used as an oogenesis marker to track hAFSCs differentiation into the OLCs.

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Bone Morphogenetic Protein-15 in the Zebrafish Ovary: Complementary Deoxyribonucleic Acid Cloning, Genomic Organization, Tissue Distribution, and Role in Oocyte Maturation

Cited by 89 publications

References 51 publications

Increase of fecundity by probiotic administration in zebrafish (Danio rerio)

Increase of fecundity by probiotic administration in zebrafish (Danio rerio)

BMPRIB and BMPRII mRNA expression levels in goat ovarian follicles and the in vitro effects of BMP-15 on preantral follicle development

BMP15 Gene Is Activated During Human Amniotic Fluid Stem Cell Differentiation into Oocyte-Like Cells

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