Bone Morphogenetic Protein-2 and Collagen Type 1 From Different Sources of Demineralized Dentine Matrix: Release Kinetic and Chemotaxis Potential for Osteoprogenitor Cells

Bao, Jibo; He, Jun; Li, Ziliang; Yang, Ruiqi; Zou, Jinyang; Ding, Yuan; Niu, Tao; Xie, Zhigang; Seriwatanachai, Dutmanee

doi:10.22159/ijap.2019.v11s1.18475

Cited by 3 publications

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“…DDM is a type of dentin that has recently been used as a bone graft material. It is obtained from extracted teeth after being demineralized, and essentially represents demineralized dentin 2 , 3 . Although the matrix of dentin and bone have different structures, they have similar chemical compositions and may induce bone formation as consistently as bone matrix 4 .…”

Section: Introductionmentioning

confidence: 99%

The healing capacity and osteogenesis pattern of demineralized dentin matrix (DDM)-fibrin glue (FG) compound

Bao,

Fu,

et al. 2023

Sci Rep

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Demineralized dentin matrix (DDM) is an osteoconductive and osteoinductive material that has been successfully used in sinus floor augmentation and alveolar ridge augmentation in clinical applications. It releases bone morphogenetic proteins (BMPs) and other growth factors, making DDM a suitable grafting material. However, the granular particle of DDM makes it difficult to anchor into the bone defect area. The aim of this study was to investigate the biological effects and osteoinductivity of the combination of DDM and Fibrin Glue (FG) at an optimal ratio on bone healing from a critical bone defect in an animal model. The mouse osteoblastic cell line (MC3T3-E1) was co-cultured with various ratios of DDM and FG to examine their effects on osteoblast proliferation and differentiation, as indicated by alkaline phosphatase (ALP) activity, osteocalcin (OC) production and mineralized nodules formation. The optimal ratio was then chosen for further study with a rabbit calvarial defective model, in which they were implanted with DDM or DDM-FG1 (1 g: 0.1 ml) and DDM-FG2 (1 g: 0.5 ml) compounds, or left blank for 2, 4, 8 and 12 weeks to investigate soft tissue and new bone regeneration. Micro-CT and histology analysis were used to evaluate the total grafting properties according to the different healing periods. The result from in vitro studies demonstrated that the ratio of 1:0.1 induced more ALP activity and mineralized nodules, while the ratio of 1: 0.5 (DDM-FG combined) induced more osteocalcin (OC) at specific time points. In the animal model, the 3D new bone volume in all DDM-FG treatment groups was significantly greater than that in the blank group at 2, 4, 8 and 12 weeks. Furthermore, the new bone volume was greater in DDM-FG2 when compared to the other groups during the early weeks of the healing period. In histological analysis, clusters of osteoblasts were formed adjacent to the DDM particles, and newly formed bone was observed in all groups, suggesting an osteoinductive property of DDM. Moreover, the greater new collagen synthesis observed at 4 weeks suggested that early bone healing was induced in the DDM-FG2 group. This study demonstrated that at an optimal ratio, the DDM-FG compound enhances osteogenic activities and bone regeneration.

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Section: Introductionmentioning

confidence: 99%

The healing capacity and osteogenesis pattern of demineralized dentin matrix (DDM)-fibrin glue (FG) compound

Bao,

Fu,

et al. 2023

Sci Rep

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Bone regeneration property of tooth-derived bone substitute prepared chairside for periodontal bone defects: an experimental study

Zhang,

Ruangsawasdi,

Pumpaluk

et al. 2023

BMC Oral Health

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Background Periodontitis often leads to progressive destruction and loss of alveolar bone, the reconstruction of which remains difficult in periodontal therapy. As a novel bone graft material, tooth-derived bone substitute (TDBS) processed from extracted teeth has been previously reported about its osteoconductivity and promising results in bone regeneration. This study was to investigate the biological effects and bone regeneration properties of TDBS in vitro and in vivo using rat periodontal bone defect model. Methods Three groups of materials were used in the experiments: TDBS, TDBS treated with ethylene diamine tetraacetic acid (EDTA) (TDBS-E), and allogeneic bone materials. Calcium (Ca) and phosphate (P) ion dissolutions were quantified by spectrophotometer for seven days. The releases of bone morphogenetic protein-2 (BMP-2) and transforming growth factor-β1 (TGF-β1) were identified by enzyme-linked immunosorbent assay (ELISA). Human osteoblast proliferation, migration, and differentiation were detected by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, cell counting, alkaline phosphatase activity (ALP), and alizarin red staining (ARS), respectively. Furthermore, the osteogenic effects of TDBS on periodontal furcation bone defects were evaluated at eight weeks postoperatively using micro-computed tomography (Micro-CT) and histological analysis. Results The dissolution of both Ca and P ions in TDBS increased over time. The BMP-2 released from TDBS was significantly higher than that from TDBS-E and allografts, while the TGF-β1 release from TDBS and TDBS-E groups was higher than that in the allografts. The TDBS-E group could induce the highest level of osteoblast proliferation compared to other groups. Cell migration with allografts co-culture was significantly induced compared to the blank control. However, all groups demonstrated similar positive effects on osteoblast differentiation. Furthermore, in the periodontal model, all materials could effectively enhance bone regeneration in the furcation defect. Conclusions The TDBS prepared chairside as an autogenous bone graft, demonstrating osteoinductivity, which enhances the osteogenic biological characteristics. Therefore, TDBS is suggested as an economical and biocompatible material for periodontal bone regeneration.

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The healing capacity and osteogenesis pattern of Demineralized Dentin Matrix (DDM)-Fibrin Glue (FG) compound

Bao

et al. 2023

Preprint

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Demineralized dentin matrix (DDM) is an osteoconductive and osteoinductive material, which was successfully performed in sinus floor augmentation and alveolar ridge augmentation in clinical application. It releases bone morphogenetic protein (BMP) and other growth factors which make DDM a suitable grafting material. However, the granular particle of DDM makes it difficult to anchor into the bone defect area. The aim of this study was to investigate biological effects as well as an osteoinductivity of the combination of DDM and Fibrin Glue (FG) at an optimal ratio on bone healing from critical bone defect in an animal model. Mouse osteoblastic cell line (MC3T3-E1) was co-cultured with various ratios of DDM and FG to examine their effects on osteoblast proliferation and differentiation indicated by alkaline phosphatase (ALP) activity, osteocalcin (OC) production and mineralized nodules formation. The optimal ratio was then chosen to further study with rabbit calvarial defective model which they were implanted with DDM or DDM-FG1 (1:0.1) and DDM-FG2 (1:0.5) compounds (w/v) or blank for 2, 4, 8 and 12weeks for investigating soft tissue and new bone regeneration. Micro-CT and histology analysis were used to evaluate the total grafting properties according to the different healing periods. The result from in vitro studies demonstrated that the ratio of 1:0.1 induced more ALP activity, mineralized nodules at the same time, 1: 0.5 (DDM-FG combined) induced more Osteocalcin (OC) at given days. In animal model, the 3D new bone volume in all DDM-FG treatment were significantly greater than blank group at 2, 4, 8 and 12 weeks. Furthermore, the new bone volume was greater in DDM-FG2 when compared to other groups at early week of the healing period. In histological analysis, numbers of osteoblasts were formed as a cluster adjacent to the DDM particles where we could observe a newly formed bone in all groups suggesting an osteoinductive property of DDM. Moreover, the greater new collagen synthesis observed at 4 weeks suggested that the early bone healing was induced in a group treated with DDM-FG2. This study demonstrates that at optimal ratio of DDM-FG compound enhances the osteogenic activities and bone regeneration.

show abstract

Bone Morphogenetic Protein-2 and Collagen Type 1 From Different Sources of Demineralized Dentine Matrix: Release Kinetic and Chemotaxis Potential for Osteoprogenitor Cells

Cited by 3 publications

References 0 publications

The healing capacity and osteogenesis pattern of demineralized dentin matrix (DDM)-fibrin glue (FG) compound

The healing capacity and osteogenesis pattern of demineralized dentin matrix (DDM)-fibrin glue (FG) compound

Bone regeneration property of tooth-derived bone substitute prepared chairside for periodontal bone defects: an experimental study

The healing capacity and osteogenesis pattern of Demineralized Dentin Matrix (DDM)-Fibrin Glue (FG) compound

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