Bone Morphogenetic Protein 2-Coated Porous Poly-L-lactic Acid Scaffolds: Release Kinetics and Induction of Pluripotent C3H10T1/2 Cells

Kim, Hyun D.; Smith, Jonathan G.; Valentini, Robert F.

doi:10.1089/ten.1998.4.35

Cited by 18 publications

(7 citation statements)

References 30 publications

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“…The pore sizes obtained with those conditions range between 100 and 400 Am. Those pore dimensions are within the range of sizes that lead to the best results for bone cell culture and bone tissue ingrowth, as reported previously [31][32][33]. The density of the specimens was measured in order to confirm the qualitative porosity assessment of the scaffolds by SEM.…”

Section: Morphology Of the Porous Structuresupporting

confidence: 65%

The morphology, mechanical properties and ageing behavior of porous injection molded starch-based blends for tissue engineering scaffolding

Neves

Kouyumdzhiev

Reis

2005

Materials Science and Engineering: C

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One important parameter in the tissue engineering of hard tissues is the scaffold. A scaffold is a support in which cells are seeded and that should create the adequate environment for the cells to attach and proliferate. Furthermore the scaffold should allow the flow of an appropriate culture media, providing nutrients to the cells and simultaneously removing the metabolites resulting from the cells activity. One of the possibilities is to obtain solid foamed structures that will enable the cells to attach, spread into the inner surfaces and start to produce extracellular matrix. Ideally, if the scaffold is produced from a biodegradable material, it should degrade at a pace that is in phase with the formation of the new tissue.In this work it was studied the production of porous structures from biodegradable polymers for use as scaffolds for bone tissue engineering. Two materials were studied, starch compounded with poly(ethylene-vinyl-alcohol) (SEVA-C) and starch with poly(lactic acid) (SPLA). The porous structures were obtained by injection molding with a blowing agent to control the porosity, interconnectivity and degradation rate. In previous attempts, the current starch compounds proved to be very difficult to process by this method. This study includes the characterization of the mechanical properties, water absorption and of the degradation kinetics of the 3-D porous structures.Two starch-based biodegradable 3D porous structures were successfully processed in conventional injection molding and the foaming was obtained by means of the use of a blowing agent. The mechanical properties are very promising as well as the improved degradation kinetics when compared with the synthetic polymers alone, although the degree of porosity and of interconnectivity needs to be improved in further work. D

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Section: Morphology Of the Porous Structuresupporting

confidence: 65%

The morphology, mechanical properties and ageing behavior of porous injection molded starch-based blends for tissue engineering scaffolding

Neves

Kouyumdzhiev

Reis

2005

Materials Science and Engineering: C

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“…in both collagen gels and polylactic acid scaffolds. 38 It is likely that the remainder of the rhBMP-2 retained on the Hyaff-11 scaffolds would continue to be released in a slow, sustained manner beyond 28 days. The high retention of rhBMP-2 observed, combined with the slow, sustained release of rhBMP-2 from Hyaff-11 scaffolds suggest a strong rhBMP-2 interaction with Hyaff-11 that result in a slow diffusion based release.…”

Section: Discussionmentioning

confidence: 99%

Retention and activity of BMP‐2 in hyaluronic acid‐based scaffolds in vitro

Kim

Valentini

2001

J. Biomed. Mater. Res.

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177

115

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Bone morphogenetic protein-2 (BMP-2) delivered in a suitable implantable matrix has the potential to repair local skeletal defects by inducing new bone formation from undifferentiated pluripotent stem cells resident in host tissue. In this study, we examined in vitro the potential of a derivatized hyaluronic acid (Hyaff-11) scaffold as a delivery vehicle for recombinant human BMP-2 (rhBMP-2) in bone and cartilage repair therapies. Hyaff-11 scaffolds were fabricated using a phase inversion/particulate leaching method and soak-loaded with rhBMP-2. In vitro release kinetics of rhBMP-2, demonstrated using enzyme-linked immunosorbant assay and alkaline phosphatase (ALP) assay revealed a slow, sustained rhBMP-2 release during 28 days, with a cumulative release of 31.82% of the initial rhBMP-2 loaded. rhBMP-2 was released in bioactive form as demonstrated by ALP induction of pluripotent cell line, C3H10T1/2 (T1/2), down the osteoblast lineage when incubated with the release supernatants. rhBMP-2 retention in Hyaff-11 scaffolds was greater than that from collagen gels, which released most of the initially loaded rhBMP-2 by 14 days. rhBMP-2-loaded Hyaff-11 scaffolds were also seeded with T1/2 cells and evaluated at 3, 7, 14, and 28 days for viability and expression of osteoblast phenotype. Cells remained viable throughout the study and expressed a time- and dose-dependent ALP and osteocalcin expression in the rhBMP-2 groups. Based on these observations, Hyaff-11 scaffolds may be suitable delivery systems for rhBMP-2 in bone/cartilage repair because of their ability to retain rhBMP-2, release low levels of bioactive rhBMP-2 to the local environment in a sustained manner, and stimulate differentiation of pluripotent stem cells.

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“…Cloning and expression of bone morphogenetic proteins (BMPs) has stimulated research on osteogenesis. BMP‐2 has been shown to be one of the most potent members of the BMP family for the induction of bone formation in vivo and has been studied using a variety of delivery systems including collagen,2 porous hydroxyapatite composites,3 Si‐Ca‐P xerogels,4 and poly‐L‐lactic acid scaffolds 5. Mesenchymal stem cells have been transduced with an adenoviral vector carrying the BMP‐2 gene; this induced their differentiation in vitro and bone formation in vivo 6.…”

Section: Introductionmentioning

confidence: 99%

“…BMP-2 has been shown to be one of the most potent members of the BMP family for the induction of bone formation in vivo and has been studied using a variety of delivery systems including collagen, 2 porous hydroxyapatite composites, 3 Si-Ca-P xerogels, 4 and poly-L-lactic acid scaffolds. 5 Mesenchymal stem cells have been transduced with an adenoviral vector carrying the BMP-2 gene; this induced their differentiation in vitro and bone formation in vivo. 6 In addition, the majority of the in vitro studies in which BMP-2 has been used involved supplementation of the medium with BMP-2 in soluble form.…”

Section: Introductionmentioning

confidence: 99%

Bone morphogenetic protein‐2 decorated silk fibroin films induce osteogenic differentiation of human bone marrow stromal cells

Karageorgiou

Meinel

Hofmann

et al. 2004

J Biomedical Materials Res

306

204

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Bone morphogenetic protein (BMP)-2 has a critical role in bone formation and regeneration. Therefore, the ability to immobilize this molecule in certain matrices may be crucial in bone tissue engineering. Using carbodiimide chemistry, BMP-2 was directly immobilized on silk fibroin films. Whereas human bone marrow stromal cells cultured on unmodified silk fibroin films in the presence of osteogenic stimulants exhibited little if any osteogenesis, the same cells cultured on BMP-2 decorated films in the presence of osteogenic stimulants differentiated into an osteoblastic lineage as assessed by their significantly elevated alkaline phosphatase activity, calcium deposition, and higher transcript levels of collagen type I, bone sialoprotein, osteopontin, osteocalcin, BMP-2, and cbfa1. Using cell culture inserts, it was demonstrated that differentiation was induced by the immobilized protein and not by protein released into the culture medium. Comparison with a similar amount of medium-supplemented BMP-2, where no additional protein was added with medium changes, showed that delivery of BMP-2 immobilized on the biomaterial surface was more efficient than soluble delivery. The results illustrate that BMP-2 covalently coupled on silk biomaterial matrices retains biological function in vitro based on the induction of osteogenic markers in seeded bone marrow stromal cells.

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Bone Morphogenetic Protein 2-Coated Porous Poly-L-lactic Acid Scaffolds: Release Kinetics and Induction of Pluripotent C3H10T1/2 Cells

Cited by 18 publications

References 30 publications

The morphology, mechanical properties and ageing behavior of porous injection molded starch-based blends for tissue engineering scaffolding

The morphology, mechanical properties and ageing behavior of porous injection molded starch-based blends for tissue engineering scaffolding

Retention and activity of BMP‐2 in hyaluronic acid‐based scaffolds in vitro

Bone morphogenetic protein‐2 decorated silk fibroin films induce osteogenic differentiation of human bone marrow stromal cells

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