Bone morphogenetic protein‐7 regulates differentially the mRNA expression of bone morphogenetic proteins and their receptors in rat achilles and patellar tendon cell cultures

Abstract

Previous animal studies have suggested that certain bone morphogenetic proteins (BMPs) may be useful therapeutically in treating tendon healing. To better understand the relationship among the different BMPs in the healing process, we initiated the present study to examine the effects of a member of the BMP family, BMP-7 (also called Osteogenic Protein-1) on the temporal and spatial expression patterns of other BMPs and the BMP receptors in cell cultures of adult rat Achilles and Patellar tendons. Cultures from both tendon types expressed detectable but variable levels of biochemical markers characteristics of tendons. RNAs coding for type II collagen and transcription factors Six1, Scleraxis, and Tendin were detected in both types of cultures. Distinct patterns of expression of several BMP members and their receptors were observed in these cultured cells and BMP-7 exerted differential effects on their expression. The findings may have implications in the treatment of different tendon injuries with BMPs.

BMP-2 and BMP-7 affect human rotator cuff tendon cells in vitro

“…59 Furthermore, proliferation and viability of tendon cells (originating from the bovine flexor tendon) and synthesis of DNA, collagen, and proteoglycans were increased dose-dependently after BMP-7 treatment. 61 In our study, the maximum effect was observed for a BMP-7 dosage of 1000 ng/mL This finding is in contrast to Yeh et al, 63 who observed declining effects beyond 50 to 100 ng/mL, and Tsai et al 59 (>400 ng/mL). However, several variables may account for a different response between these studies, such as different cell origin (animal patellar/medial collateral ligament cells vs human RC tenocyte-like cells), culture conditions or growth factor origin.…”

“…Cell proliferation and COL-I expression of tendon cell cultures (originating from rodent Patellar and Achilles tendons) were affected dose-dependently. 63 Proliferation and expression of several markers of rodent collateral ligament cells were enhanced, whereas messenger ribonucleic acid levels of COL-I increased only slightly. 59 Furthermore, proliferation and viability of tendon cells (originating from the bovine flexor tendon) and synthesis of DNA, collagen, and proteoglycans were increased dose-dependently after BMP-7 treatment.…”

“…36,39 It furthermore stimulated cell proliferation in animal ligament or tendon cell cultures dose-and timedependently. 59,63 It also increased production of collagen type I (COL-I), 61 which is the main component of the tendon ECM. 3 The aim of this study is to investigate whether the growth factors BMP-2 and BMP-7, either alone or in combination, can positively affect human RC tenocytes in terms of stimulating cell activity and COL-I production and expression of several markers.…”

Advances in biology and mechanics of rotator cuff repair

“…In vitro studies showed promising results for the tissue engineering and repair of bone [143], cartilage [148], skin [19], nerve [66], myocardium [5] and tendon [28].…”

Biologics in Achilles tendon healing and repair: a review

“…Animal studies have shown the ability of BMP-7 to induce cartilage and bone formation as well as promote tendon healing and repair in rats [79]. On the contrary, in a review conducted by Aspenberg [3], BMP-7 was said to reduce the strength of tendon and predominantly support bone growth at the site of the injured tendon.…”