Bone Neoformation Induced by Low-Level Laser and Methylene Blue Suggests Early Ossification in Rats

Oliveira, Fabiano Luiz Dulce de; Nagato, Akinori Cardozo; Aarestrup, Fernando Monteiro; Aarestrup, Beatriz Julião Vieira

doi:10.34172/jlms.2022.48

Cited by 4 publications

(3 citation statements)

References 39 publications

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“…The combined therapy also enhanced radiopacity, suggesting improved bone healing. The study suggests that MB with LLLT may stimulate bone repair and could be beneficial for bone healing [ 23 ]. Using a rat model of osteoarthritis (OA), intra-articular MB injections significantly inhibited synovitis (inflammation), relieved pain, and prevented cartilage degradation.…”

Section: Introductionmentioning

confidence: 99%

Targeting mitochondrial dysfunction using methylene blue or mitoquinone to improve skeletal aging

Poudel,

Frikha-Benayed,

Ruff

et al. 2024

Aging

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Methylene blue (MB) is a well-established antioxidant that has been shown to improve mitochondrial function in both in vitro and in vivo settings. Mitoquinone (MitoQ) is a selective antioxidant that specifically targets mitochondria and effectively reduces the accumulation of reactive oxygen species. To investigate the effect of long-term administration of MB on skeletal morphology, we administered MB to aged (18 months old) female C57BL/J6 mice, as well as to adult male and female mice with a genetically diverse background (UM-HET3). Additionally, we used MitoQ as an alternative approach to target mitochondrial oxidative stress during aging in adult female and male UM-HET3 mice. Although we observed some beneficial effects of MB and MitoQ in vitro , the administration of these compounds in vivo did not alter the progression of age-induced bone loss. Specifically, treating 18-month-old female mice with MB for 6 or 12 months did not have an effect on age-related bone loss. Similarly, long-term treatment with MB from 7 to 22 months or with MitoQ from 4 to 22 months of age did not affect the morphology of cortical bone at the mid-diaphysis of the femur, trabecular bone at the distal-metaphysis of the femur, or trabecular bone at the lumbar vertebra-5 in UM-HET3 mice. Based on our findings, it appears that long-term treatment with MB or MitoQ alone, as a means to reduce skeletal oxidative stress, is insufficient to inhibit age-associated bone loss. This supports the notion that interventions solely with antioxidants may not provide adequate protection against skeletal aging.

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Section: Introductionmentioning

confidence: 99%

Targeting mitochondrial dysfunction using methylene blue or mitoquinone to improve skeletal aging

Poudel,

Frikha-Benayed,

Ruff

et al. 2024

Aging

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“…In addition, attempts are being made to optimize bone regeneration through physical methods, promoting more effective morphophysiological rehabilitation in a shorter period. One of the recent techniques with reports of success in this area is low-level laser therapy-LLLT [49][50][51]. LLLT in bone tissue has been shown to be effective mainly in modulating the inflammatory response and accelerating the tissue repair process [52][53][54].…”

Section: Introductionmentioning

confidence: 99%

Photobiomodulation Therapy Improves Repair of Bone Defects Filled by Inorganic Bone Matrix and Fibrin Heterologous Biopolymer

Vigliar,

Marega,

Duarte

et al. 2024

Bioengineering

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Biomaterials are used extensively in graft procedures to correct bone defects, interacting with the body without causing adverse reactions. The aim of this pre-clinical study was to analyze the effects of photobiomodulation therapy (PBM) with the use of a low-level laser in the repair process of bone defects filled with inorganic matrix (IM) associated with heterologous fibrin biopolymer (FB). A circular osteotomy of 4 mm in the left tibia was performed in 30 Wistar male adult rats who were randomly divided into three groups: G1 = IM + PBM, G2 = IM + FB and G3 = IM + FB + PBM. PBM was applied at the time of the experimental surgery and three times a week, on alternate days, until euthanasia, with 830 nm wavelength, in two points of the operated site. Five animals from each group were euthanized 14 and 42 days after surgery. In the histomorphometric analysis, the percentage of neoformed bone tissue in G3 (28.4% ± 2.3%) was higher in relation to G1 (24.1% ± 2.91%) and G2 (22.2% ± 3.11%) at 14 days and at 42 days, the percentage in G3 (35.1% ± 2.55%) was also higher in relation to G1 (30.1% ± 2.9%) and G2 (31.8% ± 3.12%). In the analysis of the birefringence of collagen fibers, G3 showed a predominance of birefringence between greenish-yellow in the neoformed bone tissue after 42 days, differing from the other groups with a greater presence of red-orange fibers. Immunohistochemically, in all experimental groups, it was possible to observe immunostaining for osteocalcin (OCN) near the bone surface of the margins of the surgical defect and tartrate-resistant acid phosphatase (TRAP) bordering the newly formed bone tissue. Therefore, laser photobiomodulation therapy contributed to improving the bone repair process in tibial defects filled with bovine biomaterial associated with fibrin biopolymer derived from snake venom.

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“…Research results showed that particular laser irradiation stimulated fibroblast proliferation without impairing procollagen synthesis. Also, since LLLT can modulate inflammatory processes in a dose-dependent manner, it can significantly reduce acute inflammatory pain (9,10). However, according to some studies, LLLT can reduce pain but does not affect accelerating wound healing.…”

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confidence: 99%

Low-Level Laser Therapy Effects on Reducing Surgical Complications and Wound Infection after Cesarean Section

KAHKHAIE,

KEIKHAIE,

NASAB

et al. 2023

Maedica

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Background: One of the main problems in cesarean section cases is represented by postoperative pain that can cause delay in recovery and intense complications for patients. Therefore, the present study evaluates the effect and quality of treating infectious tissue and surgical complications using low-level lasers after cesarean section.Methods: In this clinical study, 240 candidates for the same type of surgery using the spinal anesthesia technique, were randomly divided into a laser treatment group and a control group. Finally, the surgical incision in the laser group was irradiated by laser (810 nm). Furthermore, the same method was used for the control group. The first request for analgesia, total analgesic consumption and severity of postoperative pain by visual analogue scale (VAS) were monitored in all patients for 24 hours.Findings: The 240 participants in the present study were equally allocated (1:1 ratio) to the case and control groups. There was a significant difference in average age (P=0.013) between subjects of the two study groups, with controls having a lower average age than participants in the case group based on the obtained results. However, the body mass index did not significantly differ between the two groups (p=0.1). The average use of painkillers (a painkiller is a pill or other form of drug that reduces or stops physical pain), suture removal time, and postoperative pain on days 3, 7, 14, and six weeks after surgery were lower in the case group than in the control group. The obtained difference was statistically significant (p<0.001). Conclusion:According to the findings of this study, using low-level laser therapy with cluster current (wavelength 810 nm and power 300 mW and power density for low-level laser 4 W/cm2) can help reduce postoperative pain and the need for painkillers.

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Bone Neoformation Induced by Low-Level Laser and Methylene Blue Suggests Early Ossification in Rats

Cited by 4 publications

References 39 publications

Targeting mitochondrial dysfunction using methylene blue or mitoquinone to improve skeletal aging

Targeting mitochondrial dysfunction using methylene blue or mitoquinone to improve skeletal aging

Photobiomodulation Therapy Improves Repair of Bone Defects Filled by Inorganic Bone Matrix and Fibrin Heterologous Biopolymer

Low-Level Laser Therapy Effects on Reducing Surgical Complications and Wound Infection after Cesarean Section

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