Bone regeneration by low-level laser therapy and low-intensity pulsed ultrasound therapy in the rabbit calvarium

Açar, Abdullah; Yolcu, Ümit; Altındiş, Sedat; Gul, Mehmet; Alan, Hilal; Malkoç, Sıddık

doi:10.1016/j.archoralbio.2015.10.011

Cited by 40 publications

(26 citation statements)

References 31 publications

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“…Similarly, prosthetic devices are plagued by the potential risk of infection and extrusion (3). Due to these limitations, a number of experimental studies have aimed to improve the healing of calvarial bone defects (1,4–7). Studies investigating various antioxidants including propolis (8), Nigella sativa (9), Ginkgo biloba (10) and Ankaferd BloodStopper (ABS) (11) have been performed to assess their effects on bone healing.…”

Section: Introductionmentioning

confidence: 99%

Effects of Ankaferd BloodStopper on bone healing in an ovariectomized osteoporotic rat model

Ezirganlı

Kazancioğlu

Açar

et al. 2017

Experimental and Therapeutic Medicine

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Bone defects resulting from oncological surgical resections, congenital facial anomalies, trauma or infection represent a significant and common clinical problem. The present study aimed to evaluate the effects of a commercially-available medicinal plant extract product, Ankaferd BloodStopper (ABS), on bone healing. The present study was performed on 24 female ovariectomized (OVX) rats. A defect on each rat calvarium was created using a trephine burr prior to the rats being divided into two groups. Defects were grafted with a gelatin sponge soaked with normal saline (control group) or plant extract (experimental group). Half of the animals were sacrificed after 2 weeks and the others after 4 weeks. In the control group, the defects were not filled with regenerated bone. By contrast, in the experimental group, all defect areas had an increased amount of regenerated bone and connective tissue. Osteoblastic activity appeared to be greater in the experimental group however, osteoclastic activity was observed to be higher in the control group. At 2 and 4 weeks, there was a significant difference in the amount of newly regenerated bone observed in the experimental group compared with the control group (P<0.05). Therefore, the results of the present study indicated that local ABS application had a positive effect on bone healing in the OVX rat model.

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Section: Introductionmentioning

confidence: 99%

Effects of Ankaferd BloodStopper on bone healing in an ovariectomized osteoporotic rat model

Ezirganlı

Kazancioğlu

Açar

et al. 2017

Experimental and Therapeutic Medicine

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“…; Acar et al . ). A previous study showed that infrared LLLT applied to surgically created bone defects in rats shortened the bone healing process by stimulating the modulation of the initial inflammatory response, resulting in quicker restoration of normal status (Pretel et al .…”

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confidence: 97%

Low‐level laser therapy (780 nm) combined with collagen sponge scaffold promotes repair of rat cranial critical‐size defects and increases TGF‐β, FGF‐2, OPG/RANK and osteocalcin expression

Oliveira

Araújo

Júnior

et al. 2017

Int J Experimental Path

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The aim of this study was to evaluate the effect of collagen sponge scaffold (CSS) implantation associated with low-level laser therapy (LLLT) on repairing bone defects. A single 5-mm cranial defect was surgically created in forty Wistar rats, which then received one of the following four interventions (n = 10 per group): no treatment (G0); bone defect implanted with collagen sponge scaffold (CSS) alone (G1); defect treated with low-level laser therapy (LLLT) (wavelength 780 nm; total energy density 120 J/cm ; power 50 mW) alone (G2); and CSS associated with LLLT treatment (G3). After surgery, animals in each group were euthanized at 21 days and 30 days (n = 5 per euthanasia time group). Bone formation was monitored by X-ray imaging analysis. Biopsies were collected and processed for histological analysis and immunohistochemical evaluation of transforming growth factor-beta (TGF-β), fibroblast growth factor-2 (FGF-2), osteoprotegerin (OPG) and receptor activator of nuclear factor ƙ (RANK). Osteocalcin (OCN) was detected by immunofluorescence analysis. Compared to the G0 group, defects in the 30-day G3 group exhibited increased bone formation, both by increase in radiopaque areas (P < 0.01) and by histomorphometric analysis (P < 0.001). The histopathological analysis showed a decreased number of inflammatory cells (P < 0.001). The combined CCS + LLLT (G3) treatment also resulted in the most intense immunostaining for OPG, RANK, FGF-2 and TGF-β, and the most intense and diffuse OCN immunofluorescent labelling at 30 days postsurgery (G3 vs. G0 group, P < 0.05). Therefore, the use of CCS associated with LLLT could offer a synergistic advantage in improving the healing of bone fractures.

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“…Recent studies have reported that exposure to low‐intensity pulsed ultrasound (LIPUS) accelerates tissue regeneration as a result of enhanced blood circulation, resulting in an acceleration of wound or fracture healing and induction of angiogenesis . Numerous phenomenological studies have shown that a variety of cellular events occur in injured tissues following exposure to LIPUS.…”

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confidence: 99%

Low‐intensity pulsed ultrasound accelerates nerve regeneration following inferior alveolar nerve transection in rats

Sato

Motoyoshi

Shinoda

et al. 2016

European J Oral Sciences

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Inferior alveolar nerve (IAN) injury, which is frequently caused by orofacial surgery or trauma, induces sensory loss in orofacial regions innervated by the IAN. However, no effective treatment for orofacial sensory loss currently exists. We determined whether sensory loss in facial skin above the mental foramen following IAN transection was recovered by exposure of the transected IAN to low-intensity pulsed ultrasound (LIPUS). Inferior alveolar nerve transection (IANX) was performed in 7-wk-old male Sprague-Dawley rats. On day 7 after IANX, the effect of daily LIPUS (from day 0) on the transected IAN, in terms of sensitivity to mechanical stimulation of the facial skin above the mental foramen, was examined. Moreover, the number of trigeminal ganglion (TG) neurons innervating the facial skin above the mental foramen of rats with IANX treated daily with LIPUS was counted using the retrograde neurotracing technique. Daily exposure of the transected IAN to LIPUS significantly promoted recovery of the head-withdrawal threshold in response to mechanical stimulation of the facial skin above the mental foramen, and the number of TG neurons innervating the facial skin above mental foramen was significantly increased in rats with IANX treated daily with LIPUS compared with sham or LIPUS-unexposed rats. Daily treatment of stumps of the transected IAN with LIPUS facilitated morphological and functional regeneration, suggesting that LIPUS is an effective and novel therapy for IAN injury.

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Bone regeneration by low-level laser therapy and low-intensity pulsed ultrasound therapy in the rabbit calvarium

Cited by 40 publications

References 31 publications

Effects of Ankaferd BloodStopper on bone healing in an ovariectomized osteoporotic rat model

Effects of Ankaferd BloodStopper on bone healing in an ovariectomized osteoporotic rat model

Low‐level laser therapy (780 nm) combined with collagen sponge scaffold promotes repair of rat cranial critical‐size defects and increases TGF‐β, FGF‐2, OPG/RANK and osteocalcin expression

Low‐intensity pulsed ultrasound accelerates nerve regeneration following inferior alveolar nerve transection in rats

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