Bone Regeneration in Rabbit Calvarial Defects Using Prgf and Adipose-Derived Stem Cells: Histomorphometrical Analysis

Stumbras, Artūras; Kuliešius, Povilas; Darinskas, Adas; Kubilius, Ričardas; Zigmantaitė, Vilma; Juodžbalys, Gintaras

doi:10.2217/rme-2019-0123

Cited by 7 publications

(3 citation statements)

References 78 publications

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“…Meanwhile, studies showed that the degradation of polylactic acid (PLA) biopolyester materials is relatively fast, while the degradation of polyhydroxyalkanoate (PHA) is relatively slow [20]. And human adipose derived stem cells (hADSCs) as seed cells are also a potential candidate for osteogenesis differentiation [21][22][23][24], which have been obtained more easily from subcutaneous tissue and widely employed in engineered tissue. These studies inspired us to design a novel long-acting BMPs release system based on PLA and PHA biopolymer NPs for the induction of osteogenic differentiation of hADSCs.…”

Section: Introductionmentioning

confidence: 99%

Osteogenic differentiation system based on biopolymer nanoparticles for stem cells in simulated microgravity

et al. 2021

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An efficient long-term intracellular growth factor release system in simulated microgravity for osteogenic differentiation was prepared based on polylactic acid (PLA) and polyhydroxyalkanoate (PHA) nanoparticles (NPs) for loading of bone morphogenetic protein 2 (BMP2) and bone morphogenetic protein 7 (BMP7) (defined as sB2-PLA-NPs and sB7-PHA-NPs), respectively, associated with osteogenic differentiation of human adipose derived stem cells (hADSCs). On account of soybean lecithin (SL) as biosurfactants, sB2-PLA-NPs and sB7-PHA-NPs had a high encapsulation efficiency (>80%) of BMPs and uniform small size (<100 nm), and showed a different slow-release to provide BMP2 in early stage and BMP7 in late stages of osteogenic differentiation within 20 d, due to degradation rate of PLA and PHA in cells. After uptake into hADSCs, by comparison with single sB2-PLA-NPs or sB7-PHA-NPs, the Mixture NPs compound of sB2-PLA-NP and sB7-PHA-NP with a mass ratio of 1:1, can well-promote ALP activity, expression of OPN and upregulated related osteo-genes. Directed osteo-differentiation of mixture NPs was similar to result of sustained free-BMP2 and BMP7-supplying (sFree-B2&B7) in simulated microgravity, which demonstrated the reliability and stability of Mixture NPs as a long-term osteogenic differentiation system in space medicine and biology in future.

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Section: Introductionmentioning

confidence: 99%

Osteogenic differentiation system based on biopolymer nanoparticles for stem cells in simulated microgravity

et al. 2021

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“…After activation with calcium ions [ 35 ], a dense fibrin rich matrix is formed. When placed in an osseous defect, cells will invade and migrate along the PRGF scaffold [ 21 , 36 , 37 , 38 ], to achieve healing [ 38 , 39 ]. PRGF has been demonstrated to support re-vascularization of mucosa and bone, modulate the inflammation, significantly speed bone healing, and maintain bone volume after surgical interventions [ 35 , 40 , 41 , 42 , 43 ].…”

Section: Discussionmentioning

confidence: 99%

Adjunctive Plasma Rich in Growth Factors in the Treatment of Osteomyelitis and Large Odontogenic Cysts Prior to Successful Implant Rehabilitation: Case Report

DuVal,

Alkhraisat

2023

Dentistry Journal

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Osteomyelitis of the jaws presents a clinical challenge to conventional treatment, often requiring multiple surgical interventions resulting in significant deformity and presenting significant problems to satisfactory rehabilitation. While benign odontogenic cysts, such as the radicular cyst, are generally predictably treated, they can cause significant localized bone destruction and thus can present significant problems in satisfactory rehabilitation. In this case report, patients were treated combining conventionally appropriate surgical debridement and oral antibiotics with adjunctive plasma rich in growth factors (PRGF). Patients showed a complete soft tissue and osseous regeneration to their pre-pathologic state, with successful implant rehabilitation. PRGF appears to be highly successful in minimizing or eliminating osseous deformities normally associated with conventional treatment of osteomyelitis of the jaw and treatment of large odontogenic cysts. Future trials must be performed to confirm these results in comparison to controls using conventional treatment alone.

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“…Although a few studies have explored the regenerative potential of terminally differentiated cells, e.g., osteoblasts [63,64], most research on cranial bone healing has been conducted with adult stem cells, mainly adipose-and bone marrow-derived stem cells, but also with muscle-, periodontal ligament-, neural crest-, cranial suture-and human dental pulp-derived stem cells, among others [65][66][67][68][69][70][71][72][73][74][75][76][77][78][79]. Collectively, most studies have reported the superior ability of adult stem cells to heal critical size calvarial defects compared with implantation of carriers or scaffolds lacking cells [65,66,73,75,[77][78][79]. A study conducted on rabbit calvaria showed that the regeneration ability of bone marrow-derived stem cells is similar to that achieved by autologous bone [80].…”

Section: Research On Cell-based Strategiesmentioning

confidence: 99%

A Narrative Review of Cell-Based Approaches for Cranial Bone Regeneration

et al. 2022

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Current cranial repair techniques combine the use of autologous bone grafts and biomaterials. In addition to their association with harvesting morbidity, autografts are often limited by insufficient quantity of bone stock. Biomaterials lead to better outcomes, but their effectiveness is often compromised by the unpredictable lack of integration and structural failure. Bone tissue engineering offers the promising alternative of generating constructs composed of instructive biomaterials including cells or cell-secreted products, which could enhance the outcome of reconstructive treatments. This review focuses on cell-based approaches with potential to regenerate calvarial bone defects, including human studies and preclinical research. Further, we discuss strategies to deliver extracellular matrix, conditioned media and extracellular vesicles derived from cell cultures. Recent advances in 3D printing and bioprinting techniques that appear to be promising for cranial reconstruction are also discussed. Finally, we review cell-based gene therapy approaches, covering both unregulated and regulated gene switches that can create spatiotemporal patterns of transgenic therapeutic molecules. In summary, this review provides an overview of the current developments in cell-based strategies with potential to enhance the surgical armamentarium for regenerating cranial vault defects.

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Bone Regeneration in Rabbit Calvarial Defects Using Prgf and Adipose-Derived Stem Cells: Histomorphometrical Analysis

Cited by 7 publications

References 78 publications

Osteogenic differentiation system based on biopolymer nanoparticles for stem cells in simulated microgravity

Osteogenic differentiation system based on biopolymer nanoparticles for stem cells in simulated microgravity

Adjunctive Plasma Rich in Growth Factors in the Treatment of Osteomyelitis and Large Odontogenic Cysts Prior to Successful Implant Rehabilitation: Case Report

A Narrative Review of Cell-Based Approaches for Cranial Bone Regeneration

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