Bone toughness at the molecular scale: A model for fracture toughness using crosslinked osteopontin on synthetic and biogenic mineral substrates

Cavelier, Sacha; Dastjerdi, Ahmad Khayer; McKee, Marc D.; Barthelat, François

doi:10.1016/j.bone.2018.02.022

Cited by 26 publications

(21 citation statements)

References 41 publications

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“…Interestingly, many of the non-collagenous proteins that produce fracture resistance (toughness) in bone are highly phosphorylated peptides that are also involved in load dissipation, as well as interfacial adhesion, between the organic and inorganic phases of bone [42]. Osteopontin is a non-collagenous component of the osteoid with large amounts of (>10% of total amino acids) phosphorylated serine residues [43] that increases the fracture toughness of bones [44,45,46]. Osteopontin also produces an adhesive effect at the atomic to nanoscale [29,47,48] by crosslinking via ionic bonding with calcium [42,47,49].…”

Section: Introductionmentioning

confidence: 99%

Adhesive Cements That Bond Soft Tissue Ex Vivo

et al. 2019

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The aim of the present study was to evaluate the soft tissue bond strength of a newly developed, monomeric, biomimetic, tissue adhesive called phosphoserine modified cement (PMC). Two types of PMCs were evaluated using lap shear strength (LSS) testing, on porcine skin: a calcium metasilicate (CS1), and alpha tricalcium phosphate (αTCP) PMC. CS1 PCM bonded strongly to skin, reaching a peak LSS of 84, 132, and 154 KPa after curing for 0.5, 1.5, and 4 h, respectively. Cyanoacrylate and fibrin glues reached an LSS of 207 kPa and 33 kPa, respectively. αTCP PMCs reached a final LSS of ≈110 kPa. In soft tissues, stronger bond strengths were obtained with αTCP PMCs containing large amounts of amino acid (70–90 mol%), in contrast to prior studies in calcified tissues (30–50 mol%). When αTCP particle size was reduced by wet milling, and for CS1 PMCs, the strongest bonding was obtained with mole ratios of 30–50% phosphoserine. While PM-CPCs behave like stiff ceramics after setting, they bond to soft tissues, and warrant further investigation as tissue adhesives, particularly at the interface between hard and soft tissues.

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Section: Introductionmentioning

confidence: 99%

Adhesive Cements That Bond Soft Tissue Ex Vivo

et al. 2019

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“…Such a feature, which accounts for the increased resilience and high work of fracture of biomineralized collagen-based tissues ( 35 ), may be attributed to the cross-linking of HPAA, which acts as cohesive/adhesive “binders” for the collagen/mineral composite hybrid. Similar to cross-linked osteopontin found in the bone ( 36 ), the bound HPAA may provide a binding layer between the intrafibrillarly mineralized collagen and the extrafibrillar minerals, which enhances interfacial adhesion and the fracture toughness of the material.…”

Section: Resultsmentioning

confidence: 90%

Contribution of biomimetic collagen-ligand interaction to intrafibrillar mineralization

et al. 2019

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Contemporary models of intrafibrillar mineralization mechanisms are established using collagen fibrils as templates without considering the contribution from collagen-bound apatite nucleation inhibitors. However, collagen matrices destined for mineralization in vertebrates contain bound matrix proteins for intrafibrillar mineralization. Negatively charged, high–molecular weight polycarboxylic acid is cross-linked to reconstituted collagen to create a model for examining the contribution of collagen-ligand interaction to intrafibrillar mineralization. Cryogenic electron microscopy and molecular dynamics simulation show that, after cross-linking to collagen, the bound polyelectrolyte caches prenucleation cluster singlets into chain-like aggregates along the fibrillar surface to increase the pool of mineralization precursors available for intrafibrillar mineralization. Higher-quality mineralized scaffolds with better biomechanical properties are achieved compared with mineralization of unmodified scaffolds in polyelectrolyte-stabilized mineralization solution. Collagen-ligand interaction provides insights on the genesis of heterogeneously mineralized tissues and the potential causes of ectopic calcification in nonmineralized body tissues.

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“…They discovered the presence of a non-fibrillar organic matrix based on non-collagenous proteins (NCP) mainly composed of osteopontin (OPN), which acts as a glue that holds the mineralized fibrils together. Recently, Cavalier et al [70] discovered an important mechanism suggesting that OPN crosslinking enhances the interfacial organic-inorganic adhesion, hence increases the fracture toughness of bone. The effectiveness of this mechanism increases with the presence of Ca 2+ ions.…”

Section: Structure-functionmentioning

confidence: 99%

Hierarchical Biomineralization: from Nature's Designs to Synthetic Materials for Regenerative Medicine and Dentistry

Elsharkawy

Mata

2018

Adv Healthcare Materials

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Biomineralization is a highly dynamic, yet controlled, process that many living creatures employ to develop functional tissues such as tooth enamel, bone, and others. A major goal in materials science is to create bioinspired functional structures based on the precise organization of building blocks across multiple length scales. Therefore, learning how nature has evolved to use biomineralization could inspire new ways to design and develop synthetic hierarchical materials with enhanced functionality. Toward this goal, this review dissects the current understanding of structure-function relationships of dental enamel and bone using a materials science perspective and discusses a wide range of synthetic technologies that aim to recreate their hierarchical organization and functionality. Insights into how these strategies could be applied for regenerative medicine and dentistry are also provided.

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Bone toughness at the molecular scale: A model for fracture toughness using crosslinked osteopontin on synthetic and biogenic mineral substrates

Cited by 26 publications

References 41 publications

Adhesive Cements That Bond Soft Tissue Ex Vivo

Adhesive Cements That Bond Soft Tissue Ex Vivo

Contribution of biomimetic collagen-ligand interaction to intrafibrillar mineralization

Hierarchical Biomineralization: from Nature's Designs to Synthetic Materials for Regenerative Medicine and Dentistry

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