Boosting biomolecular interactions through DNA origami nano-tailored biosensing interfaces

Rutten, Iene; Daems, Devin; Lammertyn, Jeroen

doi:10.1039/c9tb02439e

Cited by 14 publications

(14 citation statements)

References 51 publications

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“…(Fig. 4) could contribute to overcoming labour-intensive and time-consuming approaches in biosensing, leading to the development of a new generation of specific, rapid, and high-throughput analytical platforms [72].…”

Section: Origami-based Structure Design and Mechanismsmentioning

confidence: 99%

Novel nucleic acid origami structures and conventional molecular beacon–based platforms: a comparison in biosensing applications

2021

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Nucleic acid nanotechnology designs and develops synthetic nucleic acid strands to fabricate nanosized functional systems. Structural properties and the conformational polymorphism of nucleic acid sequences are inherent characteristics that make nucleic acid nanostructures attractive systems in biosensing. This review critically discusses recent advances in biosensing derived from molecular beacon and DNA origami structures. Molecular beacons belong to a conventional class of nucleic acid structures used in biosensing, whereas DNA origami nanostructures are fabricated by fully exploiting possibilities offered by nucleic acid nanotechnology. We present nucleic acid scaffolds divided into conventional hairpin molecular beacons and DNA origami, and discuss some relevant examples by focusing on peculiar aspects exploited in biosensing applications. We also critically evaluate analytical uses of the synthetic nucleic acid structures in biosensing to point out similarities and differences between traditional hairpin nucleic acid sequences and DNA origami. Graphical abstract

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Section: Origami-based Structure Design and Mechanismsmentioning

confidence: 99%

Novel nucleic acid origami structures and conventional molecular beacon–based platforms: a comparison in biosensing applications

2021

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“…Therefore, there is a continuous need for improvement and development of more efficient tools. DNA origami-based technology has the potential to revolutionize the development of a new generation of highly specific, fast, high throughput analytical devices with a single-molecule sensitivity for bio-medical applications [21,42,43].…”

Section: Dna Origami-based Structures Used For Biomolecular Sensingmentioning

confidence: 99%

“…Many approaches use fluorophores within immunoassays to improve the assay sensitivity, required for early disease detection (i.e., when the disease is still asymptomatic). A recent article by Rutten et al [43], reported a bioassay to detect thrombin in only 25 min consisting of a DNA origami structure labeled with aptamers and fluorophores with a LOD of 2 ± 0.2 nM. Chen et al [70] developed a sensor using an alternative DNA origami method called DNA nanoribbons.…”

Section: Fluorescence-based Sensorsmentioning

confidence: 99%

DNA Origami as Emerging Technology for the Engineering of Fluorescent and Plasmonic-Based Biosensors

et al. 2020

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DNA nanotechnology is a powerful and promising tool for the development of nanoscale devices for numerous and diverse applications. One of the greatest potential fields of application for DNA nanotechnology is in biomedicine, in particular biosensing. Thanks to the control over their size, shape, and fabrication, DNA origami represents a unique opportunity to assemble dynamic and complex devices with precise and predictable structural characteristics. Combined with the addressability and flexibility of the chemistry for DNA functionalization, DNA origami allows the precise design of sensors capable of detecting a large range of different targets, encompassing RNA, DNA, proteins, small molecules, or changes in physico-chemical parameters, that could serve as diagnostic tools. Here, we review some recent, salient developments in DNA origami-based sensors centered on optical detection methods (readout) with a special emphasis on the sensitivity, the selectivity, and response time. We also discuss challenges that still need to be addressed before this approach can be translated into robust diagnostic devices for bio-medical applications.

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“…18 Rutten et al has recently reported a unique scaffold, 3D DNA origami design, consisting of a 24-helix cylindrical bundle with ssDNA elongations on the side for DNA-mediated receptor coupling, and of a biotin-containing at the terminal region for coupling to the streptavidin-mediated microparticles surface. 19 It has a stable structure (up to 50 °C, pH 10), but the design of the 3D DNA origami structure is complicated. Furthermore, the DNA aptamers are immobilized on the sides of the cylindrical bundle, so they cannot be displayed vertically to the sensor surfaces.…”

Section: Introductionmentioning

confidence: 99%

Bio-nanocapsules for oriented immobilization of DNA aptamers on aptasensors

Iijima

Yamada

Nakano

et al. 2022

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Boosting biomolecular interactions through DNA origami nano-tailored biosensing interfaces

Abstract: Nano-tailored DNA origami designs nanostructure the bioreceptor layer of encoded microparticles in an innovative microfluidic platform, hereby boosting biomolecular interactions.

Cited by 14 publications

References 51 publications

Novel nucleic acid origami structures and conventional molecular beacon–based platforms: a comparison in biosensing applications

Novel nucleic acid origami structures and conventional molecular beacon–based platforms: a comparison in biosensing applications

DNA Origami as Emerging Technology for the Engineering of Fluorescent and Plasmonic-Based Biosensors

Bio-nanocapsules for oriented immobilization of DNA aptamers on aptasensors

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