Boosting Electrochemiluminescence Immunoassay Sensitivity via Co–Pt Nanoparticles within a Ti₃C₂ MXene-Modified Single Electrode Electrochemical System on Raspberry Pi

Abstract

Point-of-care testing plays a crucial role in diagnostics within resourcepoor areas, necessitating the utilization of portable and user-friendly devices. The adaptation of biosensors for point-of-care applications requires careful considerations, such as miniaturization, cost-effectiveness, and streamlined sample processing. In recent years, the electrochemiluminescence (ECL) immunoassay has gained significant attention due to its visual detection capabilities and ability to facilitate high-throughput analysis. However, the development of a practical and cost-effective ECL device remains a challenging task. This study presents the development of an integrated MXenemodified single-electrode electrochemical system (SEES) for visual and high-throughput ECL immunoassays incorporating a Raspberry Pi system. The SEES was designed by affixing a plastic sticker with multiple perforations onto a single carbon ink screenprinted electrode, which operates based on a resistance-induced potential difference. Leveraging the excellent adsorption and bioaffinity properties of the carbon ink screenprinted electrode, effective immobilization of antibodies was achieved. Furthermore, the incorporation of Co−Pt nanoparticles enhanced the ECL intensity and electron transfer kinetics, enabling the sensitive detection of SARS-CoV-2. The developed system comprised 18 individual reaction cells, allowing for simultaneous analysis while maintaining sample isolation. Impressively, the system achieved a remarkable minimum virus detection limit of 10 −14 g mL −1 , accompanied by a high R 2 value of 0.9798. These findings highlight the promising potential of our developed system for efficient point-of-care testing in resource-limited settings.

Unmasking the Electrochemiluminescence Properties of Ternary Mn/Fe/Co Metals Doped Porous g-C3N4 Fiber-like Nanostructure

“…The multifaceted merits of ECL, including its heightened sensitivity, broad dynamic response range, rapid response speed, and minimal background signal, have propelled it to become a prominent analytical method in diverse domains such as clinical diagnosis, bioanalysis, food analysis, and environmental monitoring. − Realizing the full potential of ECL for these applications necessitates continuous research efforts devoted to developing proficient ECL luminophores that are characterized by elevated efficiency and stability.…”

Spatial-Potential-Color-Resolved Bipolar Electrode Electrochemiluminescence Biosensor Using a CuMoOx Electrocatalyst for the Simultaneous Detection and Imaging of Tetracycline and Lincomycin

“…Signal amplification is a necessary method to improve the sensitivity of target detection. − At present, the signal amplification strategies of BPE-ECL sensors mainly focus on redox-active species replacement, , enzyme catalysis amplification, , nanomaterial-based amplification, , and nucleic acid amplification . Some electrocatalyst nanomaterials with excellent catalytic ability and conductivity can significantly promote the electron transfer of BPE, thus obtaining a higher ECL signal. , For example, Xu et al used electrocatalyst Pt-tipped Au nanorods to catalyze the electrochemical reduction of H 2 O 2 to increase the Faraday current of BPE to achieve ECL signal amplification of anodic luminophore cyclometalated iridium­(III) complex .…”

Colorimetric and fluorescent dual-biosensor based on zirconium and preasodium metal-organic framework (zr/pr MOF) for miRNA-191 detection

“…Metal-organic framework-based biosensors provide a number of benefits over other materials, including a larger loading capacity, conjugated π-electron systems [ 21 ], tunable porosity [ 22 ], enhanced surface area [ 23 ], and lower fabrication costs [ 24 ]. The efficacy of these biosensors is highly dependent on the detection methods employed, such as electrochemical (EC) [ 25 ], electrochemiluminescence (ECL) [ 26 ], fluorescence (FL) [ 27 ], and colorimetry [ 28 ]. Fluorescence-based detection assays and colorimetric techniques have been employed to develop a simple, extremely sensitive approaches with distinct color variation [ 29 ] that does not require expensive equipment [ 30 ].…”