Boosting expression level of plectasin in recombinant Pichia pastoris via 2A self-processing peptide assembly

Liang, Xingxing; Jiang, Hong; Si, Xiandong; Qi, Xin; Meng, Di; Chen, Peng; Mao, Xiangzhao

doi:10.1007/s00253-022-11942-x

Cited by 5 publications

(2 citation statements)

References 38 publications

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“…In previous studies, we found that the enzyme fusion strategy reduced the cordycepin titer of the engineered strains through rDNA-mediated integration. Other protein engineering strategies, such as 2A peptide and protein scaffolds, have been successfully applied to optimize the synthesis of natural products in microbial cell factories. − The upstream 2A peptide residues have been shown to promote the synthesis of α-bisabolol . In addition, the titer of β-carotene reached 2.02 mg/g DCW by exchanging the gene order of key enzymes in the polycistronic 2A peptide construct .…”

Section: Resultsmentioning

confidence: 99%

Increased Cordycepin Production in Yarrowia lipolytica Using Combinatorial Metabolic Engineering Strategies

et al. 2023

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As the first nucleoside antibiotic discovered in fungi, cordycepin, with its various biological activities, has wide applications. At present, cordycepin is mainly obtained from the natural fruiting bodies of Cordyceps militaris. However, due to long production periods, low yields, and low extraction efficiency, harvesting cordycepin from natural C. militaris is not ideal, making it difficult to meet market demands. In this study, an engineered Yarrowia lipolytica YlCor-18 strain, constructed by combining metabolic engineering strategies, achieved efficient de novo cordycepin production from glucose. First, the cordycepin biosynthetic pathway derived from C. militaris was introduced into Y. lipolytica. Furthermore, metabolic engineering strategies including promoter, protein, adenosine triphosphate, and precursor engineering were combined to enhance the synthetic ability of engineered strains of cordycepin. Fermentation conditions were also optimized, after which, the production titer and yields of cordycepin in the engineered strain YlCor-18 under fed-batch fermentation were improved to 4362.54 mg/L and 213.85 mg/g, respectively, after 168 h. This study demonstrates the potential of Y. lipolytica as a cell factory for cordycepin synthesis, which will serve as the model for the green biomanufacturing of other nucleoside antibiotics using artificial cell factories.

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Section: Resultsmentioning

confidence: 99%

Increased Cordycepin Production in Yarrowia lipolytica Using Combinatorial Metabolic Engineering Strategies

et al. 2023

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“…However, because it is a fusion construct, downstream cleaving of the fusion tag was necessary. Fish [ 44 ], human [ 45 ] and fungal [ 46 ] peptides have also successfully been produced recombinantly in P. pastoris (Table 1 ), showing evidence that it can be a useful alternative to the most used expression system for recombinant production ( E. coli ). Saccharmoyces cerevisiae is another yeast-based system that has been used for the expression of HDPs [ 47 , 48 ], albeit less than P. pastoris .…”

Section: Cell Factories For Recombinant Hdp Productionmentioning

confidence: 99%

The future of recombinant host defense peptides

et al. 2022

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The antimicrobial resistance crisis calls for the discovery and production of new antimicrobials. Host defense peptides (HDPs) are small proteins with potent antibacterial and immunomodulatory activities that are attractive for translational applications, with several already under clinical trials. Traditionally, antimicrobial peptides have been produced by chemical synthesis, which is expensive and requires the use of toxic reagents, hindering the large-scale development of HDPs. Alternatively, HDPs can be produced recombinantly to overcome these limitations. Their antimicrobial nature, however, can make them toxic to the hosts of recombinant production. In this review we explore the different strategies that are used to fine-tune their activities, bioengineer them, and optimize the recombinant production of HDPs in various cell factories.

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Heterologous expression of the novel dimeric antimicrobial peptide LIG in Pichia pastoris

Zhao,

Li,

et al. 2024

Journal of Biotechnology

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Boosting expression level of plectasin in recombinant Pichia pastoris via 2A self-processing peptide assembly

Cited by 5 publications

References 38 publications

Increased Cordycepin Production in Yarrowia lipolytica Using Combinatorial Metabolic Engineering Strategies

Increased Cordycepin Production in Yarrowia lipolytica Using Combinatorial Metabolic Engineering Strategies

The future of recombinant host defense peptides

Heterologous expression of the novel dimeric antimicrobial peptide LIG in Pichia pastoris

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