BOP1– A Key Player of Ribosomal Biogenesis

Borah, Nabajyoti; Nukala, Krishna Madhav; Vadlapudi, Varahalarao; Gubbala, Satya Prakash; Amanchy, Ramars

doi:10.18520/cs/v117/i3/422-433

Cited by 1 publication

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“…The 39 proteins that were still >90% positive for 13 C 6 -Lys included many proteins with key cellular functions, such as TWINKLE, a mitochondrial DNA helicase that is essential for mitochondrial DNA replication 7 ; TTC19, which is essential for the assembly and function of complex III of the mitochondrial respiratory chain 8 ; BOP1, a protein that is essential for ribosome assembly 9 ; and HIRA, a histone chaperone (Fig. 1c ).…”

Section: Resultsmentioning

confidence: 99%

“…Ovaries were collected from the female progeny at eleven time points (three animals per time point) and processed for DDA MS. b, Progeny from fully 13 C 6 -Lys labelled pregnant mice were fed with 13 C 6 -Lys until weaning. After the weaning period (3 weeks after birth), progeny were fed The 39 proteins that were still >90% positive for 13 C 6 -Lys included many proteins with key cellular functions, such as TWINKLE, a mitochondrial DNA helicase that is essential for mitochondrial DNA replication 7 ; TTC19, which is essential for the assembly and function of complex III of the mitochondrial respiratory chain 8 ; BOP1, a protein that is essential for ribosome assembly 9 ; and HIRA, a histone chaperone (Fig. 1c).…”

Section: Mitochondrial Proteinsmentioning

confidence: 99%

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The maintenance of oocytes in the mammalian ovary involves extreme protein longevity

Harasimov,

Gorry,

Welp

et al. 2024

Nat Cell Biol

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Women are born with all of their oocytes. The oocyte proteome must be maintained with minimal damage throughout the woman’s reproductive life, and hence for decades. Here we report that oocyte and ovarian proteostasis involves extreme protein longevity. Mouse ovaries had more extremely long-lived proteins than other tissues, including brain. These long-lived proteins had diverse functions, including in mitochondria, the cytoskeleton, chromatin and proteostasis. The stable proteins resided not only in oocytes but also in long-lived ovarian somatic cells. Our data suggest that mammals increase protein longevity and enhance proteostasis by chaperones and cellular antioxidants to maintain the female germline for long periods. Indeed, protein aggregation in oocytes did not increase with age and proteasome activity did not decay. However, increasing protein longevity cannot fully block female germline senescence. Large-scale proteome profiling of ~8,890 proteins revealed a decline in many long-lived proteins of the proteostasis network in the aging ovary, accompanied by massive proteome remodeling, which eventually leads to female fertility decline.

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Section: Resultsmentioning

confidence: 99%