Bordetella bronchiseptica promotes adherence, colonization, and cytotoxicity of Streptococcus suis in a porcine precision-cut lung slice model

Vötsch, Désirée; Willenborg, Maren; Baumgärtner, Wolfgang; Rohde, Manfred; Valentin‐Weigand, Peter

doi:10.1080/21505594.2020.1858604

Cited by 18 publications

(13 citation statements)

References 45 publications

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“…Notably, the prior infection with Bordetella bronchiseptica supported cytotoxic effects of Streptococcus suis. 146 Other infection studies on PCLS derived from farm and domestic animals have so far been restricted to nonbovine ruminants, to investigate the Jaagsiekte sheep retrovirus, 20 and respiratory disease complex in goats. 72 In addition, investigations on the pathogenesis of paramyxoviruses have been performed using PCLS of ferrets and dogs infected with canine distemper virus, macaques with measles virus, as well as cotton rats with metapneumovirus and respiratory syncytial virus.…”

Section: Precision-cut Lung Slicesmentioning

confidence: 99%

Alternatives to animal models and their application in the discovery of species susceptibility to SARS-CoV-2 and other respiratory infectious pathogens: A review

et al. 2022

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The emergence of the coronavirus disease 2019 (COVID-19) caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) inspired rapid research efforts targeting the host range, pathogenesis and transmission mechanisms, and the development of antiviral strategies. Genetically modified mice, rhesus macaques, ferrets, and Syrian golden hamsters have been frequently used in studies of pathogenesis and efficacy of antiviral compounds and vaccines. However, alternatives to in vivo experiments, such as immortalized cell lines, primary respiratory epithelial cells cultured at an air–liquid interface, stem/progenitor cell-derived organoids, or tissue explants, have also been used for isolation of SARS-CoV-2, investigation of cytopathic effects, and pathogen–host interactions. Moreover, initial proof-of-concept studies for testing therapeutic agents can be performed with these tools, showing that animal-sparing cell culture methods could significantly reduce the need for animal models in the future, following the 3R principles of replace, reduce, and refine. So far, only few studies using animal-derived primary cells or tissues have been conducted in SARS-CoV-2 research, although natural infection has been shown to occur in several animal species. Therefore, the need for in-depth investigations on possible interspecies transmission routes and differences in susceptibility to SARS-CoV-2 is urgent. This review gives an overview of studies employing alternative culture systems like primary cell cultures, tissue explants, or organoids for investigations of the pathophysiology and reverse zoonotic potential of SARS-CoV-2 in animals. In addition, future possibilities of SARS-CoV-2 research in animals, including previously neglected methods like the use of precision-cut lung slices, will be outlined.

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Section: Precision-cut Lung Slicesmentioning

confidence: 99%

Alternatives to animal models and their application in the discovery of species susceptibility to SARS-CoV-2 and other respiratory infectious pathogens: A review

et al. 2022

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“…Since the first ever use of PCLS as a model for respiratory infection with C. pneumoniae (Ebsen et al., 2002 ), an increasing number of studies have exploited PCLS to investigate how bacterial pathogens cause disease in humans. Additional studies on zoonotic pathogens, for example, Bordetella bronchiseptica , that focus on aspects of pathogenesis in the natural animal hosts have been discussed elsewhere (Vötsch et al., 2021 ).…”

Section: Pcls As Model For Human Respiratory Infectious Diseasesmentioning

confidence: 99%

Precision‐cut lung slices: A powerful ex vivo model to investigate respiratory infectious diseases

Viana

O'Kane

Schroeder

2021

Molecular Microbiology

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Respiratory infections are a leading cause of mortality worldwide. Most of the research on the underlying disease mechanisms is based on cell culture, organoid, or surrogate animal models. Although these provide important insights, they have limitations. Cell culture models fail to recapitulate cellular interactions in the lung and animal models often do not permit high-throughput analysis of drugs or pathogen isolates; hence, there is a need for improved, scalable models. Precision-cut lung slices (PCLS), small, uniform tissue slices generated from animal or human lungs are increasingly recognized and employed as an ex vivo organotypic model. PCLS retain remarkable cellular complexity and the architecture of the lung, providing a platform to investigate respiratory pathogens in a near-native environment. Here, we review the generation and features of PCLS, their use to investigate the pathogenesis of viral and bacterial pathogens, and highlight their potential to advance respiratory infection research in the future.

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“…Alterations of mucociliary clearance can also be bacterially mediated. Colonization of porcine lung tissues with Bordetella bronchiseptica led to reduced ciliary action and enhanced susceptibility to colonization and infection with Streptococcus suis (133).…”

Section: Polymicrobial Impacts On Acquisitionmentioning

confidence: 99%

Polymicrobial Interactions Operative during Pathogen Transmission

Rowe

Rosch

2021

mBio

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Pathogen transmission is a key point not only for infection control and public health interventions but also for understanding the selective pressures in pathogen evolution. The “success” of a pathogen lies not in its ability to cause signs and symptoms of illness but in its ability to be shed from the initial hosts, survive between hosts, and then establish infection in a new host. Recent insights have shown the importance of the interaction between the pathogen and both the commensal microbiome and coinfecting pathogens on shedding, environmental survival, and acquisition of infection. Pathogens have evolved in the context of cooperation and competition with other microbes, and the roles of these cooperations and competitions in transmission can inform novel preventative and therapeutic strategies. IMPORTANCE Transmission of pathogens from one host to another is an essential event in pathogenesis. Transmission is driven by factors intrinsic to the host and to the pathogen. In addition, transmission is altered by interactions of the pathogen with the commensal microbiota of the host and coinfecting pathogens. Recent insights into these interactions have shown both enhanced and reduced transmission efficiencies depending on the makeup of the polymicrobial community. This review will discuss polymicrobial interactions during shedding from the initial host, time in the environment, and acquisition by the new host.

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Bordetella bronchiseptica promotes adherence, colonization, and cytotoxicity of Streptococcus suis in a porcine precision-cut lung slice model

Cited by 18 publications

References 45 publications

Alternatives to animal models and their application in the discovery of species susceptibility to SARS-CoV-2 and other respiratory infectious pathogens: A review

Alternatives to animal models and their application in the discovery of species susceptibility to SARS-CoV-2 and other respiratory infectious pathogens: A review

Precision‐cut lung slices: A powerful ex vivo model to investigate respiratory infectious diseases

Polymicrobial Interactions Operative during Pathogen Transmission

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