Bordetella pertussis iron regulated proteins as potential vaccine components

Hayes, Jimena Álvarez; Erben, Esteban; Lamberti, Yanina Andrea; Principi, Guido Mariano; Maschi, Fabricio Alejandro; Ayala, Miguel Ángel; Rodrı́guez, Marı́a Eugenia

doi:10.1016/j.vaccine.2013.05.072

Cited by 26 publications

(13 citation statements)

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“…Immunization with rAfuA induced an IgG specific response that was already detectable 21 days after the first immunization. As previously found , repeated immunization led to a booster response of specific IgG anti‐rAfuA (log titer: 6.7 ± 0.25) with a balanced IgG1/IgG2a response (Fig. ).…”

Section: Resultsmentioning

confidence: 99%

“…As previously described, Escherichia coli BL21‐CodonPlus (DE3)‐RIL transformed with plasmid pET28/His6‐AfuA was used in expression experiments. rAfuA was obtained as described in and adsorbed with sepharose–polymyxin B (Sigma, St. Louis, MO, USA) to eliminate LPS contamination.…”

Section: Methodsmentioning

confidence: 99%

“…Mice were immunized as previously described . Briefly, groups of seven BALB/c mice were immunized intraperitoneally with 10 μg of rAfuA protein obtained as previously described and emulsified in complete FA. Twenty one days after the first immunization, a booster dose with the same amount of recombinant protein emulsified in incomplete FA was given.…”

Section: Methodsmentioning

confidence: 99%

“…Antibody titers were determined by ELISA, as previously described with minor modifications. Briefly, twofold diluted samples were assayed in 96‐well polystyrene microtiter plates that were coated with 10 µg/mL of recombinant protein in coating buffer (0.5 M carbonate buffer, pH 9.5), wild type B. parapertussis grown either in SS or SS‐Fe (OD:1) in PBS or BppΔwbm grown in SS‐Fe (OD:1) in PBS.…”

Section: Methodsmentioning

confidence: 99%

“…Using a proteomic approach, we have previously detected antigenic proteins exposed on the surface of B. pertussis under conditions of iron limitation , an environmental condition that human pathogens face during host infection . Two of these proteins, AfuA (BP1605) and IRP1‐3 (BP1152), which are involved in vital iron uptake systems and expressed in the infective phenotype, proved to be good targets for opsonins and highly protective against infection . In the present study, a homolog of AfuA was found to be expressed in iron‐starved B. parapertussis and its potential as a protective antigen against B. parapertussis infection was investigated.…”

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confidence: 99%

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A recombinant iron transport protein from Bordetella pertussis confers protection against Bordetella parapertussis

Hayes

Oviedo

Valdez

et al. 2017

Microbiology and Immunology

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Whooping cough, which is caused by Bordetella pertussis and B. parapertussis, is a reemerging disease. New protective antigens are needed to improve the efficacy of current vaccines against both species. Using proteomic tools, it was here found that B. parapertussis expresses a homolog of AfuA, a previously reported new vaccine candidate against B. pertussis. It was found that this homolog, named AfuA , is expressed during B. parapertussis infection, exposed on the surface of the bacteria and recognized by specific antibodies induced by the recombinant AfuA cloned from B. pertussis (rAfuA). Importantly, the presence of the O-antigen, a molecule that has been found to shield surface antigens on B. parapertussis, showed no influence on antibody recognition of AfuA on the bacterial surface. The present study further showed that antibodies induced by immunization with the recombinant protein were able to opsonize B. parapertussis and promote bacterial uptake by neutrophils. Finally, it was shown that this antigen confers protection against B. parapertussis infection in a mouse model. Altogether, these results indicate that AfuA is a good vaccine candidate for acellular vaccines protective against both causative agents of whooping cough.

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Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

mentioning

confidence: 99%

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A recombinant iron transport protein from Bordetella pertussis confers protection against Bordetella parapertussis

Hayes

Oviedo

Valdez

et al. 2017

Microbiology and Immunology

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Shotgun proteome analysis of Bordetella pertussis reveals a distinct influence of iron availability on the bacterial metabolism, virulence, and defense response

et al. 2015

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One of the mechanisms involved in host immunity is the limitation of iron accessibility to pathogens, which in turn provokes the corresponding physiological adaptation of pathogens. This study reports a gel-free nanoLC-MS/MS-based comparative proteome analysis of Bordetella pertussis grown under iron-excess and iron-depleted conditions. Out of the 926 proteins covered 98 displayed a shift in their abundance in response to low iron availability. Forty-seven of them were found to be increased in level while 58 were found with decreased protein levels under iron starvation. In addition to proteins previously reported to be influenced by iron in B. pertussis, we observed changes in metabolic proteins involved in fatty acid utilization and poly-hydroxybutyrate production. Additionally, many bacterial virulence factors regulated by the BvgAS two-component system were found at decreased levels in response to iron limitation. These results, together with the increased production of proteins potentially involved in oxidative stress resistance, seem to indicate that iron starvation provokes changes in B. pertussis phenotype that might shape host-pathogen interaction.

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New Pertussis Vaccines: A Need and a Challenge

Hozbor

2019

Advances in Experimental Medicine and Biology

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Bordetella pertussis iron regulated proteins as potential vaccine components

Cited by 26 publications

References 50 publications

A recombinant iron transport protein from Bordetella pertussis confers protection against Bordetella parapertussis

A recombinant iron transport protein from Bordetella pertussis confers protection against Bordetella parapertussis

Shotgun proteome analysis of Bordetella pertussis reveals a distinct influence of iron availability on the bacterial metabolism, virulence, and defense response

New Pertussis Vaccines: A Need and a Challenge

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