The soil water retention curve (SWRC) is one of the most fundamental characteristics of unsaturated soils. Because unsaturated soils are subjected to a range of natural processes and engineered treatments, a thorough understanding is needed of how their retention properties change when exposed to each separate treatment. In recent years, several biological treatment methods, such as microbial-induced calcite precipitation (MICP), have been introduced as environmentally friendly techniques. This study investigates the effect of biological treatment by means of Bacillus sphaericus on the SWRC of both coarse-grained and fine-grained soil samples. Pore fluid pH, X-ray diffraction, and scanning electron microscopy tests are used to better delineate the mechanisms affecting biologically induced alterations of SWRCs. Results showed that higher bacterial concentrations produced higher air-entry values in coarse-grained soils. However, the fine-grained samples showed an initial increase followed by a decrease in the air-entry value when the bacterial concentration increased. Changes in the soil porous structure and double-layer thickness are thought to be main reasons causing the different behavior of fine-grained soils.