Boric acid and boronic acids inhibition of pigeonpea urease

Reddy, K. R. C.; Kayastha, Arvind M.

doi:10.1080/14756360600638147

Cited by 24 publications

(1 citation statement)

References 30 publications

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“…Several classes of compounds are known to inhibit ureases [3], including amides and esters of phosphoric acid [5, 10], thiols [11], hydroxamic acids [12], phosphinic and thiophosphinic acids [13], boric and boronic acids [14, 15], phosphate [16], heavy metal ions [17, 18], bismuth compounds [19], quinones [20, 21], and to a lesser extent H 2 O 2 [22], as well as L -ascorbic and dehydroascorbic acid in the presence of Fe 3+ ions [23]. Due to their toxicity, however, only few of the compounds may classify as medicinal and agricultural agents.…”

Section: Introductionmentioning

confidence: 99%

Temperature- and pressure-dependent stopped-flow kinetic studies of jack bean urease. Implications for the catalytic mechanism

2012

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Urease, a Ni-containing metalloenzyme, features an activity that has profound medical and agricultural implications. The mechanism of this activity, however, has not been as yet thoroughly established. Accordingly, to improve its understanding, in this study we analyzed the steady-state kinetic parameters of the enzyme (jack bean), KM and kcat, measured at different temperatures and pressures. Such an analysis is useful as it provides information on the molecular nature of the intermediate and transition states of the catalytic reaction. We measured the parameters in a noninteracting buffer using a stopped-flow technique in the temperature range 15–35 °C and in the pressure range 5–132 MPa, the pressure-dependent measurements being the first of their kind performed for urease. While temperature enhanced the activity of urease, pressure inhibited the enzyme; the inhibition was biphasic. Analyzing KM provided the characteristics of the formation of the ES complex, and analyzing kcat, the characteristics of the activation of ES. From the temperature-dependent measurements, the energetic parameters were derived, i.e. thermodynamic ΔHo and ΔSo for ES formation, and kinetic ΔH≠ and ΔS≠ for ES activation, while from the pressure-dependent measurements, the binding ΔVb and activation volumes were determined. The thermodynamic and activation parameters obtained are discussed in terms of the current proposals for the mechanism of the urease reaction, and they are found to support the mechanism proposed by Benini et al. (Structure 7:205–216; 1999), in which the Ni–Ni bridging hydroxide—not the terminal hydroxide—is the nucleophile in the catalytic reaction.Graphical abstract

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Section: Introductionmentioning

confidence: 99%

Temperature- and pressure-dependent stopped-flow kinetic studies of jack bean urease. Implications for the catalytic mechanism

2012

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Arylboronic Acids and their Myriad of Applications Beyond Organic Synthesis

et al. 2020

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Boron organometallic compounds are mainly known for their great value in organic synthesis to construct biaryls in Suzuki couplings, for arylations of olefins, for C-S, CO , and C-N couplings or as organometallic catalysts. Herein, using arylboronic acids (Ar-B(OH) 2) as a illustrative case, we review the applications of these highly versatile molecules which expands much beyond their commonly perceived uses. Seeing primarily as "mere" intermediates in organic synthesis and organometallic catalysis, this review shows many examples of their uses in other areas of chemistry and seeks therefore to contribute to awaken scientists to the wide range of new possibilities that have been opened to boronorganic compounds, which have acted indeed as very versatile and attractive chemicals with much desirable characteristics such as safety, stability, and [a] N. de

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DFT simulations and Vibrational spectra of 4‐chloro and 4‐bromophenylboronic acid molecules

Kurt

2008

J Raman Spectroscopy

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The experimental and theoretical vibrational spectra of 4-chloro-and 4-bromophenylboronic acids (abbreviated as 4Clpba and 4Brpba) were studied. The Fourier transform Raman and Fourier transform infrared (FTIR) spectra of 4Clpba and 4Brpba molecules were recorded in the solid phase. The structural and spectroscopic analyses of the molecules were made by using Hartree-Fock and density functional harmonic calculations. In both 4Clpba and 4Brpba only one form was most stable using B3LYP level with the 6-311++G(d,p) basis set. Selected experimental bands were assigned and characterized on the basis of the scaled theoretical wavenumbers by their total energy distribution (TED). Finally, geometric parameters as well as infrared (IR) and Raman bands were compared with the experimental data of the molecules.

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Boric acid and boronic acids inhibition of pigeonpea urease

Cited by 24 publications

References 30 publications

Temperature- and pressure-dependent stopped-flow kinetic studies of jack bean urease. Implications for the catalytic mechanism

Temperature- and pressure-dependent stopped-flow kinetic studies of jack bean urease. Implications for the catalytic mechanism

Arylboronic Acids and their Myriad of Applications Beyond Organic Synthesis

DFT simulations and Vibrational spectra of 4‐chloro and 4‐bromophenylboronic acid molecules

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