Boron increases the cell viability of mesenchymal stem cells after long-term cryopreservation

Demirci, Selami; Doğan, Ayşegül; Şişli, Burcu; Şahın, Fikrettin

doi:10.1016/j.cryobiol.2014.01.010

Cited by 31 publications

(26 citation statements)

References 36 publications

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“…hTGSCs can successfully differentiate into osteogenic and odontogenic cell lineages in vitro indicating their possible use in dental tissue engineering approaches (Yalvac et al, 2009a;Do gan et al, 2012;TaS slı et al, 2013;Demirci et al, 2014). However, hTGSCs may not differentiate into all cell lineages required for dental regeneration.…”

Section: Introductionmentioning

confidence: 99%

In vitro differentiation of human tooth germ stem cells into endothelial‐ and epithelial‐like cells

Doğan

Demirci

Şahın

2014

Cell Biology International

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Current clinical techniques in dental practice include stem cell and tissue engineering applications. Dental stem cells are promising primary cell source for mainly tooth tissue engineering. Interaction of mesenchymal stem cell with epithelial and endothelial cells is strictly required for an intact tooth morphogenesis. Therefore, it is important to investigate whether human tooth germ stem cells (hTGSCs) derived from wisdom tooth are suitable for endothelial and epithelial cell transformation in dental tissue regeneration approaches. Differentiation into endothelial and epithelial cell lineages were mimicked under defined conditions, confirmed by real time PCR, western blotting and immunocytochemical analysis by qualitative and quantitative methods. HUVECs and HaCaT cells were used as positive controls for the endothelial and epithelial differentiation assays, respectively. Immunocytochemical and western blotting analysis revealed that terminally differentiated cells expressed cell-lineage markers including CD31, VEGFR2, VE-Cadherin, vWF (endothelial cell markers), and cytokeratin (CK)-17, CK-19, EpCaM, vimentin (epithelial cell markers) in significant levels with respect to undifferentiated control cells. Moreover, high expression levels of VEGFR1, VEGFR2, VEGF, CK-18, and CK-19 genes were detected in differentiated endothelial and epithelial-like cells. Endothelial-like cells derived from hTGSCs were cultured on Matrigel, tube-like structure formations were followed as an indication for functional endothelial differentiation. hTGSCs successfully differentiate into various cell types with a broad range of functional abilities using an in vitro approach. These findings suggest that hTGSCs may serve a potential stem cell source for tissue engineering and cell therapy of epithelial and endothelial tissue.

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Section: Introductionmentioning

confidence: 99%

In vitro differentiation of human tooth germ stem cells into endothelial‐ and epithelial‐like cells

Doğan

Demirci

Şahın

2014

Cell Biology International

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“…Dental pulp stem cells (DPSC) were the most cryopreserved DSC (52%). DMSO has been applied as CA in 100% of the selected studies, at concentrations between 3% and 20% (16,19,20). Only 19% of the included studies performed the cryopreservation for periods higher than one year (20)(21)(22)(23).…”

Section: Resultsmentioning

confidence: 99%

“…Besides, it was possible to cryopreserve DPSC by the fast-freezing technique (-80 °C) for up to 1 year, thus eliminating the pre-freezing steps (20). Lower DMSO concentration 3% (DPSC) and 5% (Human Tooth Germ Stem Cells -HTGSC) were effective to maintain cell biological properties when cryopreserved into a magnetic field freezer (14) or by adding NaB (20 µg) to CA (19). DSC cryopreserved with 10% glycerol and 10% ethylene glycol (12,25) presented similar cell biological properties when compared to those cryopreserved with 10% DMSO.…”

Section: Resultsmentioning

confidence: 99%

Does Cryopreservation Affect the Biological Properties of Stem Cells from Dental Tissues? A Systematic Review

et al. 2016

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This systematic review evaluated if different cryopreservation protocols could affect biological properties (Cell survival rate (CSR), proliferation, differentiation, maintenance of stem cell markers) of stem cells obtained from dental tissues (DSC) post-thaw. An electronic search was carried out within PubMed and ISI Web Science by using specific keyword. Two independent reviewers read the titles and abstracts of all reports respecting predetermined inclusion/exclusion criteria. Data were extracted considering the biological properties of previously cryopreserved DSCs and previously cryopreserved dental tissues. DSCs cryopreserved as soon as possible after their isolation presents a CSR quite similar to the non-cryopreserved DSC. Dimethyl sulfoxide (DMSO) [10%] showed good results related to cell recovery post-thaw to cryopreserve cells and tissues for periods of up to 2 years. The cryopreservation of DSC in a mechanical freezer (-80°C) allows the recovery of stem cells post-thaw. The facilities producing magnetic field (MF), demand a lower concentration of cryoprotectant, but their use is not dispensable. It is possible to isolate and cryopreserve dental pulp stem cell (DPSC) from healthy and diseased vital teeth. Cryopreservation of dental tissues for late DSC isolation, combined with MF dispensability, could be valuable to reduce costs and improve the logistics to develop teeth banks.

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“…BN is found in the field of biomaterials, usually in tooth cements and cosmetic products. One of the fundamental advantages of BN application is that it enhances mechanical properties [47,48].…”

Section: Discussionmentioning

confidence: 99%

Study of the boron levels in serum after implantation of different ratios nano-hexagonal boron nitride–hydroxy apatite in rat femurs

Atila

Halıcı

Çadırcı

et al. 2016

Materials Science and Engineering: C

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Boron increases the cell viability of mesenchymal stem cells after long-term cryopreservation

Cited by 31 publications

References 36 publications

In vitro differentiation of human tooth germ stem cells into endothelial‐ and epithelial‐like cells

In vitro differentiation of human tooth germ stem cells into endothelial‐ and epithelial‐like cells

Does Cryopreservation Affect the Biological Properties of Stem Cells from Dental Tissues? A Systematic Review

Study of the boron levels in serum after implantation of different ratios nano-hexagonal boron nitride–hydroxy apatite in rat femurs

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